Vitamin-B12: Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Vitamin-B12 Level- Test Result, Unit, Normal Range, Test Method, and Clinical Significance

Introduction of Vitamin-B12 Vitamin B12 is naturally present in animal foods like fish, meat, poultry, egg, milk, and other dairy product. It is also available in foods or supplements. It requires the formation of red blood cells (RBCs) and DNA which play a key role …

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Thyroid Function Test (TFT)-Introduction, Test Result, Unit, Normal Range, Test Method, Clinical Significance, and Keynotes

Thyroid Function Test (TFT)- Test Result, Unit, Normal Range, Test Method, and Clinical Significance

Introduction of Thyroid Function Test (TFT) If you are suffering from any of the symptoms stated in the table (Differences between Hypothyroidism and Hyperthyroidism), ask for a thyroid function test (TFT). Hypothyroidism is a condition in which the thyroid-stimulating hormone (TSH) level in your body …

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Decarboxylase Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes

Decarboxylase Test-Positive and Negative Results Demonstration

Principle of Decarboxylase-Dihydrolase Test Lysine, ornithine, and arginine are the most common amino acids which use to detect an organism’s ability to decarboxylate or hydrolyze an amino acid in a decarboxylase medium, forming an amine that produces an alkaline pH. The basal medium for the …

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Methyl-α-D-glucopyranoside (MGP) Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes

Methyl-α-D-glucopyranoside (MGP) Test- Positive and Negative Results Demonstration

Introduction of Methyl-α-D-glucopyranoside (MGP)Test Methyl-α-D-glucopyranoside (MGP) test is effective for species identification of Enterococcus. Enterococcus gallinarum and Enterococcus casseliflavus are MGP test positive while Enterococcus faecalis and Enterococcus faecium are negative. Vancomycin-resistant Enterococcus faecium (VREF) is a serious nosocomial problem that is difficult to differentiate from  Enterococcus gallinarum and Enterococcus casseliflavus, while not important for purposes of control of …

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Indoxyl Acetate Disk Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes

Indoxyl Acetate Disk Test-Positive and Negative Results

Introduction of Indoxyl Acetate Disk Test  Indoxyl is a tryptophan product of putrefactive decomposition in the intestines of humans by a bacterial esterase. This Indoxyl Acetate Disk test uses to distinguish Campylobacter species. Positive tests are Campylobacter jejuni and Campylobacter coli whereas Campylobacter lari and Campylobacter fetus are negative. Principle of Indoxyl Acetate Disk Assay The …

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Microbiology Reporting Methods: Introduction, List of Templates, and Keynotes

Salmonella Typhi isolated from blood culture-Growth on Nutrient agar, blood agar and MacConkey medium, Biochemical reactions in TSI, SIM,Citrate and Urea agar, and growth on broth medium (blood culture bottle)-demonstration

Introduction of Microbiology Reporting Methods Microbiology Reporting Methods are useful to provide similar report formats from different Microbiology Laboratory platforms that should sound Microbiologically clear and concise. It should also be compatible with International Guidelines of Clinical Microbiology Practice like the Clinical & Laboratory Standards …

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TCBS Agar : Introduction, Composition,Principle, Test Requirement,Procedure, Result -Interpretation, Limitation, and Keynotes

Vibrio cholerae colony morphology on TCBS agar

Introduction of TCBS Agar Vibrio cholerae is the causative agent of cholera and Thiosulfate Citrate bile salts sucrose (TCBS) agar is a selective as well as differential culture medium used for selective isolation of this bacterium including its species from a variety of clinical and nonclinical specimens. TCBS agar …

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Dermatophyte Test Medium (DTM): Introduction, Composition, Principle, Test Requirements, Procedure, Result-Interpretation, Colony Characteristics, Modifications,Uses, and Keynotes

Dermatophytes growth on Dermatophyte Test Medium (DTM)

Introduction of Dermatophyte Test Medium (DTM) Dermatophyte Test Medium (DTM) is a selective and differential medium recommended for the cultivation and isolation of pathogenic dermatophytes. The Dermatophytes are a distinct group of fungi that infect the hair, skin, and nails in humans producing a variety of …

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