Alkaptonuria: Introduction, Symptoms, Causes, Lab Diagnosis, Treatment, and Keynotes

Alkaptonuria: Introduction, Symptoms, Causes, Lab Diagnosis, and Treatment

Introduction Alkaptonuria is an enzyme deficiency, homogentisic dioxygenase (HGD) rare inherited disorder. It is used to break down a toxic substance called homogentisic acid. Excess of homogentisic acid causes bones and cartilage to become discolored and brittle which causes later osteoarthritis, especially in the spine …

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Robertson’s Cooked Meat (RCM) Medium: Introduction, Principle, Composition, Preparation, Test Procedure, Result Interpretation, Uses and Keynotes

Robertson’s Cooked Meat (RCM) Medium Showing Saccharolytic anaerobes(red) and Proteolytic anaerobe (black)

Introduction of Robertson’s Cooked Meat (RCM) Medium Robertson’s Cooked Meat (RCM) Medium is the recommended medium for the cultivation of anaerobic as well as aerobic bacteria. It is especially for pathogenic Clostridium species from clinical, food, and water samples. It can also be used as a maintenance medium …

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Urine Casts: Introduction, Identification Features, and Clinical Significance

Granular Cast in Urine Microscopy

Introductions of Common Urinary Casts Urine casts in urine routine examinations are completed in the following steps- First request the patient to bring a urine specimen. After the arrival of the specimen in the clinical laboratory, take nearly 5 ml of urine and centrifuge it. …

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Urine Crystals: Introduction, Identification Features, and Clinical Significance

Urate Crystals or Uric acid Crystals in Urine Microscopy

Introductions of Common Urinary Crystals Urinary crystals in urine during the routine examination can appear in the following steps- First request the patient to bring a urine sample. After the arrival of the sample/specimen in the clinical laboratory, take nearly 5 ml of urine. Centrifuge it …

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Hot air oven: Introduction, Principle, Structure, Handling Procedure, Sterilization control, Uses, Advantages, and Disadvantage

Structure of Hot Air Oven and Functions

Introduction of Hot air oven A hot air oven is the most common method of sterilization in the laboratory working on dry heat. Sterilization is the process of removing or destroying all microorganisms including viruses, bacteria, and their spores from the article or surface without …

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Autoclave: Introduction, Fundamental Structures, Principle, Types, Sterilization Control, Working Mechanisms, Uses, and Precautions

Autoclave: Introduction, Fundamental structures, Principle, Types, Sterilization Control, Working Mechanisms, Uses, and Precautions

Introduction of Autoclave An autoclave is the most common method of sterilization in the laboratory working on moist heat. Sterilization is the process of removing or destroying all microorganisms including viruses, bacteria, and their spores from the article or surface without destroying its quality and …

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Incubator: Introduction, Principle, Handling Procedure, Types, Uses, and Keynotes

Blood agar and chocolate agar incubated CO2 Incubator

Introduction of Incubator The incubator is an electrical device in which the incubation process is performed which conditions an enclosure to a predetermined temperature since it provides and maintains all artificial optimal conditions for the growth of microbial culture as well as cell and tissue …

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Brain heart infusion (BHI) Broth: Introduction, Composition, Principle, Preparation, Test Procedure, Result-Interpretation, Modifications, Uses, and Keynotes

Blood inoculated BHI broth after 4 days of incubation showing bacterial growth

Introduction of BHI Broth Brain Heart Infusion (BHI) broth is a highly nutritive medium and is thus useful for cultivating a wide variety of microorganisms like aerobic and anaerobic bacteria, yeasts, and molds.  BHI broth is commonly used in blood culture work and allied pathological …

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