Common Instruments and Reagents Used in Hematology Laboratory: Introduction, List of Instruments and Reagents, and Short Description

Introduction of Common Instruments and Reagents Used in Hematology Laboratory

Hematology is an integral branch of Pathology and Laboratory Medicine that deals with the study of blood, blood-forming organs (bone marrow, thymus, spleen, lymph nodes), blood diseases like anemia, thrombocytopenia, leukopenia, leukemia, and infection indicators like typhoid (low total leukocyte count with increased lymphocytes), other bacterial infections (increased neutrophils count), parasitic infection (increased eosinophils number) and so on. Complete blood count (CBC) and erythrocyte sedimentation rate (ESR) tests are the common hematologic assays preferred by clinicians.

Common Instruments and Reagents Used in Hematology Laboratory-Introduction, List of Instruments and Reagents, Principle and Uses-
Fig. Common Instruments and Reagents Used in Hematology Laboratory

List of Common Instruments and Reagents Used in Hematology Laboratory

  • Sysmex poch-100i
  • Hematology Analyzer or Sysmex XS 1000i cell counter
  • Tube Roller Mixer or Blood Collection Mixer
  • ESR stand
  • Glass slide
  • Wright stain
  • Blood coagulometer
  • Water bath
  • Microscope
  • Improved Neubauer’s counting chamber
  • Micropipettes
  • WBC diluting fluid or Turk’s solution
  • Cedarwood oil
  • Timer or stopwatch
  • Manual Blood Cell Counter 5 Keys or Differential Cell Counter

Instruments and Reagents Short Description

Sysmex poch-100i

Sysmex pocH-100i is a 3 components separation of leukocytes (neutrophils, lymphocytes, and remaining cells mixed count) hematology analyzer which provides complete blood count without blast or other conditions (anemia, thrombocytopenia) comments.

Sysmex Cell Counter or Henatology analyzer or Sysmex pocH-100i
Fig. Sysmex Cell Counter or Hematology analyzer or Sysmex pocH-100i

Hematology Analyzer or Sysmex XS 1000i cell counter

Hematology Analyzer or Sysmex XS 1000i Cell Counter is a 5 components cell analyzer and gives the status complete blood count with five different cells of leukocytes (neutrophil, eosinophil, basophil, lymphocytes, and monocytes) and even blast and other conditions of test blood.

Hematology Analyzer or Sysmex XS 1000i cell counter
Fig. Hematology Analyzer or Sysmex XS 1000i cell counter

Tube Roller Mixer or Blood Collection Mixer

Tube Roller Mixer or Blood Collection Mixer is used for mixing blood samples and cells.

Tube Roller Mixer or Blood Collection Mixer
Fig. Tube Roller Mixer or Blood Collection Mixer

ESR stand

ESR stand: It is used to hold ESR tubes. This ESR stand is made from good quality plastic or metal and coated with enamel.

ESR stand
Fig. ESR stand

Glass slide

Glass slides are mandatory in Hematology laboratories for a variety of purposes like PBS, reticulocyte count, etc.

Glass slide
Fig. Glass slide

Wright stain

It is also called Romanowsky stain and is a mixture of methylene blue and derivates complexed by Eosin. It is a hematologic stain that clears the way for the differentiation of blood cell types.

Wright stain
Fig. Wright stain

Blood coagulometer

Blood coagulometer: It is an analyzer applied to test the coagulation efficiency of blood so as to diagnose and assess bleeding disorders.

Blood coagulometer
Fig. Blood coagulometer

Water bath

It is a laboratory constant temperature equipment, providing a heat source for
various devices that need heating and it is used to keep water at a constant temperature for
incubating test samples in a laboratory.

Water bath
Fig. Water bath

Microscope

The microscope is the common instrument used in hematology laboratories for a variety of purposes like
manual total leukocyte count, RBC count, platelets count, differential leukocyte count, reticulocyte count, etc.

Microscope
Fig. Microscope

Improved Neubauer’s counting chamber

Improved Neubauer’s counting chamber: It is the most useful for counting cells like WBCs, RBCs, platelets, spermatozoa, etc.

Improved Neubauer counting chamber
Fig. Improved Neubauer counting chamber

Micropipettes

Micropipettes are the common and essential laboratory instruments used to accurately and precisely transfer liquid in the microliter volume.

Micropipettes
Fig. Micropipettes

WBC diluting fluid or Turk’s solution

WBC diluting fluid or Turk’s solution: It is used for visualization of white blood cells(WBCs) and it contains crystal violet, acetic acid, and distilled water. The solution destroys the others cells of blood samples like red blood cells (RBCs) and platelets and stains the nuclei of the WBCs, making them easier to see and count.

WBC diluting fluid or Türk's solution
Fig. WBC diluting fluid or Türk’s solution

Cedarwood oil

Cedarwood oil is used as an immersion medium to replace the air gap which helps to correct mismatch footage and its refractive index of around 1.51 which is similar to glass. This enhances the resolution of the high-power immersion objective and increases numerical aperture (NA) by lowering refraction.

Cedarwood oil
Fig. Cedarwood oil

Timer or stopwatch

Laboratory Digital Timer is mandatory in the laboratory for a variety of steps like staining, incubating the test samples, assaying blood clotting time, bleeding time, etc. As we know that precisely measure time for procedures and laboratory work that require precise operations.  With time-sensitive products, having a faulty or inefficient timer can create all the differences in the final output.

Timer or stopwatch
Fig. Timer or stopwatch

Manual Blood Cell Counter 5 Keys or Differential Cell Counter

Manual Blood Cell Counter 5 Keys or Differential Cell Counter is common equipment used in hematology laboratories to count neutrophils, eosinophils, basophils, lymphocytes, and monocyte of white blood cells (leukocytes).

Manual Blood Cell Counter 5 Keys or Differential Cell Counter
Fig. Manual Blood Cell Counter 5 Keys or Differential Cell Counter

Contd…

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    Understanding the processes and protocols within a Professional Tenure Committee (PTC) is crucial for faculty members. This Frequently Asked Questions (FAQ) guide aims to address common queries related to PTC procedures, voting, and membership.

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    Vote justification forms provide transparency in decision-making. Members articulate their reasoning, fostering a culture of openness and ensuring that decisions are well-founded and understood by the academic community.

    2. How can absentee ballots be cast?
    To accommodate absentee voting, PTCs may implement secure electronic methods or designated proxy voters. This ensures that faculty members who cannot physically attend meetings can still contribute to decision-making processes.

    3. How will additional members of PTCs be elected in departments with fewer than four tenured faculty members?
    In smaller departments, creative solutions like rotating roles or involving faculty from related disciplines can be explored. Flexibility in election procedures ensures representation even in departments with fewer tenured faculty members.

    4. Can a faculty member on OCSA or FML serve on a PTC?
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    6. What constitutes a positive or negative vote in PTCs?
    A positive vote typically indicates approval or agreement, while a negative vote signifies disapproval or disagreement. Clear definitions and guidelines within each PTC help members interpret and cast their votes accurately.

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    A quorum, the minimum number of members required for a valid meeting, is essential for decision-making. Specific rules about quorum size are usually outlined in the PTC’s governing documents.

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    Understanding the processes and protocols within a Professional Tenure Committee (PTC) is crucial for faculty members. This Frequently Asked Questions (FAQ) guide aims to address common queries related to PTC procedures, voting, and membership.

    1. Why should members of the PTC fill out vote justification forms explaining their votes?
    Vote justification forms provide transparency in decision-making. Members articulate their reasoning, fostering a culture of openness and ensuring that decisions are well-founded and understood by the academic community.

    2. How can absentee ballots be cast?
    To accommodate absentee voting, PTCs may implement secure electronic methods or designated proxy voters. This ensures that faculty members who cannot physically attend meetings can still contribute to decision-making processes.

    3. How will additional members of PTCs be elected in departments with fewer than four tenured faculty members?
    In smaller departments, creative solutions like rotating roles or involving faculty from related disciplines can be explored. Flexibility in election procedures ensures representation even in departments with fewer tenured faculty members.

    4. Can a faculty member on OCSA or FML serve on a PTC?
    Faculty members involved in other committees like the Organization of Committee on Student Affairs (OCSA) or Family and Medical Leave (FML) can serve on a PTC, but potential conflicts of interest should be carefully considered and managed.

    5. Can an abstention vote be cast at a PTC meeting?
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    6. What constitutes a positive or negative vote in PTCs?
    A positive vote typically indicates approval or agreement, while a negative vote signifies disapproval or disagreement. Clear definitions and guidelines within each PTC help members interpret and cast their votes accurately.

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