Hafnia alvei: Introduction, and Properties

Introduction of Hafnia alvei

Hafnia alvei is a members of the Enterobacteriaceae and it is inhabited in our gastrointestinal tract and also in many animal species as part of the normal microflora. It is a Gram negative rod, non capsulated and non-sporing bacterium motile with flagella. Malignancy, trauma, and recent surgery patients are prone to H. alvei infections.

Properties of Hafnia alvei

Basic FeaturesProperties
1. Gram StainingGram Negative Rods (GNRs)
2. Spore Non-Sporing
3. CapsuleNon-Capsulated
4. Motility Motile
5. PigmentNegative
6. Growth in potassium cyanide (KCN) mediumPositive
7. Catalase testPositive
8. Oxidase testNegative
9. Nitrate reduction testPositive
10. MR (Methyl Red) testVariable
11. VP (Voges- Proskauer) assayPositive 
12. OF (Oxidative-Fermentative) testFermentative
13. Gas from GlucosePositive
14. H2S productionNegative
15. Indole formationNegative
16. Urease/ urea hydrolysis testNegative
17. Citrate/ citrate utilizationPositive
18. DNase testNegative
19. Glucose fermentationPositive 
20. Maltose fermentationPositive
21. Lactose fermentationNegative
22. Sucrose fermentationNegative
23. Xylose fermentationPositive
24. Mannitol fermentationPositive
25. Acetate UtilizationNegative
26. ONPG (β-galactosidase)Positive
27. Phenylalanine Deaminase (PDA)/PPA TestNegative
28. Lipase testNegative
29. Esculin Hydrolysis testNegative
30. Lysine Decarboxylase TestPositive
31. Ornithine Decarboxylase TestPositive
32. Arginine Dihydrolase TestNegative
Table: Properties of Hafnia alvei
Hafnia alvei (white mucoid) colony on Muller-Hinton Agar (MHA)
Fig. Hafnia alvei (white) colony on Muller-Hinton Agar (MHA)

Further Reading

  • https://www.sciencedirect.com/topics/medicine-and-dentistry/hafnia-alvei
  • Cowan & Steel’s Manual for identification of Medical Bacteria. Editors: G.I. Barron & R.K. Felthani, 3rd ed 1993, Publisher Cambridge University Press.
  • Bailey & Scott’s Diagnostic Microbiology. Editors: Bettey A. Forbes, Daniel F. Sahm & Alice S. Weissfeld, 12th ed 2007, Publisher Elsevier.
  • Mackie and Mc Cartney Practical Medical Microbiology. Editors: J.G. Colle, A.G. Fraser, B.P. Marmion, A. Simmous, 4th ed, Publisher Churchill Living Stone, New York, Melborne, Sans Franscisco 1996.
  • Textbook of Diagnostic Microbiology. Editors: Connie R. Mahon, Donald G. Lehman & George Manuselis, 3rd edition2007, Publisher Elsevier.

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