Immunology Laboratory: Introduction, List of Tests, Specimen, Testing Method, and Keynotes

Introduction of Immunology Laboratory

The Immunology Laboratory is useful for laboratory testing and clinical consultation in several broad domains including the evaluation of autoimmune disease, immunodeficiencies, immunoproliferative disorders, and allergies, as well as having responsibility for some senses of infectious disease serology. To provide absolute consulting and diagnosis for diverse clinical diseases, much work is done in collaboration with the Molecular Diagnostics, Microbiology, Virology, and Hematology laboratories.

Immunology Laboratory List of Tests, Specimen, and Testing Method

Anti-mitochondrial antibody (AMA)with titerSerum (2 ml)Chemiluminescence
immunoassay (CLIA)
ANA by IFASerum (2 ml)CLIA
ANA Panel 8 (DSDNA with TITRES, SS-A, SS-B,
Serum (2 ml)CLIA
ANA ScreenSerum (2 ml)CLIA
ANCA (Anti Neutrophil Cytoplasmic antibodies)
Serum (2 ml)CLIA
Anti-Cyclic Citrullinated Peptides (Anti-CCP) assaySerum (1 ml)CLIA
Anti-diuretic hormone/ADH/vasopressin TestWhole blood (2 ml)Radio Immunoassay (RIA)
Antigliadin antibodies (AGAs)-IgASerum (1 ml)ELISA
Antigliadin antibodies (AGAs)-IgGSerum (1 ml)ELISA
Anti-glomerular basement membrane (GBM) IgG antibodySerum (1 ml)ELISA
Anti-insulin antibodySerum (1 ml)Enzyme Immunoassay
Anti-soluble liver antigen (SLA) antibodies Serum (1 ml)Enzyme Immunoassay
Anti-thyroid peroxidase (anti-TPO) antibodiesSerum (1 ml)CLIA
Anti-Müllerian Hormone TestSerum (1 ml)CLIA
Antistreptolysin O (ASO) Antibodies Serum (2 ml)Agglutination
Anti-thyroglobulin antibody (ATG)Serum (1 ml)CLIA
Anti-(U1)snRNP antibodiesSerum (1 ml)ELISA
Monitoring breast cancer(CA 15-3, CEA)Serum (2 ml)CLIA
Cancer antigen 125 (CA 125)Serum (1 ml)CLIA
Cancer antigen  15-3 (CA  15-3)Serum (1 ml)CLIA
Cancer antigen 19-9 (CA 19-9)Serum (1 ml)CLIA
Cancer antigen 72.4 (CA 72.4)Serum (1 ml)CLIA
CalprotectinStool (100 gm)ELISA
Antineutrophil Cytoplasmic Antibodies (ANCA) TestSerum (2 ml)ELISA
Carcinoembryonic antigen (CEA) testSerum (2 ml)CLIA
Cardiolipin IgG AntibodiesSerum (1 ml)Enzyme Immunoassay
Cardiolipin IgM AntibodiesSerum (1 ml)Enzyme Immunoassay
Celiac Antibodies Profile tTG IgA, tTG IgG, DGP IgA, DGP IgG, and Total IgASerum (1-3 ml)CLIA
Clostridium difficile A/B toxinStool (10 gm)Microbiology
CortisolSerum (1-3 ml)CLIA
Immunohistochemistry (IHC) Custom Panel TestBlock (1)Immunohistochemistry (IHC)
Double-Stranded DNA (dsDNA) Antibody, IgGSerum (1-3 ml)ELISA
18 antigen ENA (Extractable Nuclear Antigen Antibodies) panelSerum (1-3 ml)ELISA
ENA-6S-ProfileSerum (1-3 ml)ELISA
Interleukin 6 (IL-6)Serum (1-3 ml)CLIA
Inhibin ASerum (1-3 ml)CLIA
Inhibin BSerum (1-3 ml)ELISA
Estradiol(E2)/Estrogen/OestrogenSerum (1-3 ml)CLIA
Free Prostate-Specific Antigen (PSA)Serum (1-3 ml)CLIA
High-sensitivity C-reactive protein (hs-CRP) testSerum (1-3 ml)Nephelometry
Immunoglobulin A (IgA)Serum (1-3 ml)Nephelometry
Immunoglobulin E (IgE), TotalSerum (1-3 ml)CLIA
Immunoglobulin G (IgG) Serum/ CSFserum/CSF (1-3 ml)Nephelometry
Immunoglobulin G4 (IgG4)Serum (1-3 ml)Nephelometry
Immunoglobulin M (IgM) Serum/ CSFserum/CSF (1-3 ml)Nephelometry
Insulin-like growth factor-1 (IGF-1) Serum (1-3 ml)CLIA
JO-1 IgGSerum (1 ml)ELISA
Liver kidney microsome type 1 antibody (anti-LKM-1 or CYP2D6 antibody) testSerum (1 ml)ELISA
Antineutrophil cytoplasmic antibodies (ANCA) testSerum (2 ml)ELISA
Phospholipid (Cardiolipin) IgGSerum (2 ml)CLIA
Phospholipid (Cardiolipin)IgMSerum (2 ml)CLIA
Plasma-Free Metanephrines AssayPlasma (3 ml)Enzyme Immunoassay
Progesterone receptor(Pgr)Serum (2 ml)CLIA
Anti-topoisomerase I (anti-Scl-70) antibodiesSerum (1 ml)Immuno Blot
Anti-Smith (Sm) IgG antibodiesSerum (1 ml)Immuno Blot
Anti-SS-A/Ro and anti-SS-B/La IgG antibodiesSerum (1 ml)Immuno Blot
Anti-Ro/SSA IgG antibodiesSerum (1 ml)Immuno Blot
Anti-SSB/La IgG antibodiesSerum (1 ml)Immuno Blot
TacrolimusSerum (3 ml)Chemiluminescent Immunoassay (CMIA)
Tissue Transglutaminase IgA (tTG-IgA) TestSerum (2 ml)Enzyme Immunoassay
Total Prostate-Specific Antigen (PSA) TestSerum (1 ml)CLIA
TSH receptor antibodies (TRAB)Serum (1 ml)CLIA
Tissue Transglutaminase (TTG)/Deamidated Gliadin Peptide (DGP)(IgA+IgG) TestSerum (2 ml)Enzyme Immunoassay
Tumour Necrosis Factor alpha (TNF alpha)Serum (1-2 ml)Enzyme Immunoassay
Urine 24 hours for VMA (Vanillylmandelic acid)24 hours of Urine (10 ml)HPLC
Table: Immunology Laboratory List of Tests, Specimen, and Testing Methods

Keynotes on Immunology Laboratory

  • The laboratory collaborates with the Immune Monitoring Core Facility in the evaluation of immunodeficiency, whether congenital or acquired through transplantation, especially in clinical trials. The laboratory is in charge of testing that is on the verge of becoming clinical — tests created there frequently make their way into full clinical trials.
  • IFA Stands for Indirect Fluorescent Antibody.
  • The calprotectin stool test is noninvasive and it is tested to determine if you have an inflammatory or noninflammatory bowel condition. Colonoscopy or sigmoidoscopy is the alternative assay of the calprotectin stool test.
  • The most common specimen container for immunology assay is a red-top tube or gel-barrier tube.
  • ENA-6S-Profile is an ELISA test system for the qualitative determination of IgG antibodies against six extractable nuclear antigens (ENA), SS-A (Ro 52, Ro 60) SS-B, Sm, RNP/Sm, Scl-70 and Jo-1 in human serum or plasma that helps to the diagnosis of inflammatory rheumatic connective tissue diseases.

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