Introduction of Immunology Laboratory
Table of Contents
The Immunology Laboratory is useful for laboratory testing and clinical consultation in several broad domains including the evaluation of autoimmune disease, immunodeficiencies, immunoproliferative disorders, and allergies, as well as having responsibility for some senses of infectious disease serology. To provide absolute consulting and diagnosis for diverse clinical diseases, much work is done in collaboration with the Molecular Diagnostics, Microbiology, Virology, and Hematology laboratories.
Immunology Laboratory List of Tests, Specimen, and Testing Method
| Test | Specimen | Method |
| Anti-mitochondrial antibody (AMA)with titer | Serum (2 ml) | Chemiluminescence immunoassay (CLIA) |
| ANA by IFA | Serum (2 ml) | CLIA |
| ANA Panel 8 (DSDNA with TITRES, SS-A, SS-B, U1-snRNP, SMITH ABS) | Serum (2 ml) | CLIA |
| ANA Screen | Serum (2 ml) | CLIA |
| ANCA (Anti Neutrophil Cytoplasmic antibodies) (P-ANCA + C-ANCA) | Serum (2 ml) | CLIA |
| Anti-Cyclic Citrullinated Peptides (Anti-CCP) assay | Serum (1 ml) | CLIA |
| Anti-diuretic hormone/ADH/vasopressin Test | Whole blood (2 ml) | Radio Immunoassay (RIA) |
| Antigliadin antibodies (AGAs)-IgA | Serum (1 ml) | ELISA |
| Antigliadin antibodies (AGAs)-IgG | Serum (1 ml) | ELISA |
| Anti-glomerular basement membrane (GBM) IgG antibody | Serum (1 ml) | ELISA |
| Anti-insulin antibody | Serum (1 ml) | Enzyme Immunoassay |
| Anti-soluble liver antigen (SLA) antibodies | Serum (1 ml) | Enzyme Immunoassay |
| Anti-thyroid peroxidase (anti-TPO) antibodies | Serum (1 ml) | CLIA |
| Anti-Müllerian Hormone Test | Serum (1 ml) | CLIA |
| Antistreptolysin O (ASO) Antibodies | Serum (2 ml) | Agglutination |
| Anti-thyroglobulin antibody (ATG) | Serum (1 ml) | CLIA |
| Anti-(U1)snRNP antibodies | Serum (1 ml) | ELISA |
| Monitoring breast cancer(CA 15-3, CEA) | Serum (2 ml) | CLIA |
| Cancer antigen 125 (CA 125) | Serum (1 ml) | CLIA |
| Cancer antigen 15-3 (CA 15-3) | Serum (1 ml) | CLIA |
| Cancer antigen 19-9 (CA 19-9) | Serum (1 ml) | CLIA |
| Cancer antigen 72.4 (CA 72.4) | Serum (1 ml) | CLIA |
| Calprotectin | Stool (100 gm) | ELISA |
| Antineutrophil Cytoplasmic Antibodies (ANCA) Test | Serum (2 ml) | ELISA |
| Carcinoembryonic antigen (CEA) test | Serum (2 ml) | CLIA |
| Cardiolipin IgG Antibodies | Serum (1 ml) | Enzyme Immunoassay |
| Cardiolipin IgM Antibodies | Serum (1 ml) | Enzyme Immunoassay |
| Celiac Antibodies Profile tTG IgA, tTG IgG, DGP IgA, DGP IgG, and Total IgA | Serum (1-3 ml) | CLIA |
| Clostridium difficile A/B toxin | Stool (10 gm) | Microbiology |
| Cortisol | Serum (1-3 ml) | CLIA |
| Immunohistochemistry (IHC) Custom Panel Test | Block (1) | Immunohistochemistry (IHC) |
| Double-Stranded DNA (dsDNA) Antibody, IgG | Serum (1-3 ml) | ELISA |
| 18 antigen ENA (Extractable Nuclear Antigen Antibodies) panel | Serum (1-3 ml) | ELISA |
| ENA-6S-Profile | Serum (1-3 ml) | ELISA |
| Interleukin 6 (IL-6) | Serum (1-3 ml) | CLIA |
| Inhibin A | Serum (1-3 ml) | CLIA |
| Inhibin B | Serum (1-3 ml) | ELISA |
| Estradiol(E2)/Estrogen/Oestrogen | Serum (1-3 ml) | CLIA |
| Free Prostate-Specific Antigen (PSA) | Serum (1-3 ml) | CLIA |
| High-sensitivity C-reactive protein (hs-CRP) test | Serum (1-3 ml) | Nephelometry |
| Immunoglobulin A (IgA) | Serum (1-3 ml) | Nephelometry |
| Immunoglobulin E (IgE), Total | Serum (1-3 ml) | CLIA |
| Immunoglobulin G (IgG) Serum/ CSF | serum/CSF (1-3 ml) | Nephelometry |
| Immunoglobulin G4 (IgG4) | Serum (1-3 ml) | Nephelometry |
| Immunoglobulin M (IgM) Serum/ CSF | serum/CSF (1-3 ml) | Nephelometry |
| Insulin-like growth factor-1 (IGF-1) | Serum (1-3 ml) | CLIA |
| JO-1 IgG | Serum (1 ml) | ELISA |
| Liver kidney microsome type 1 antibody (anti-LKM-1 or CYP2D6 antibody) test | Serum (1 ml) | ELISA |
| Antineutrophil cytoplasmic antibodies (ANCA) test | Serum (2 ml) | ELISA |
| Phospholipid (Cardiolipin) IgG | Serum (2 ml) | CLIA |
| Phospholipid (Cardiolipin)IgM | Serum (2 ml) | CLIA |
| Plasma-Free Metanephrines Assay | Plasma (3 ml) | Enzyme Immunoassay |
| Progesterone receptor(Pgr) | Serum (2 ml) | CLIA |
| Anti-topoisomerase I (anti-Scl-70) antibodies | Serum (1 ml) | Immuno Blot |
| Anti-Smith (Sm) IgG antibodies | Serum (1 ml) | Immuno Blot |
| Anti-SS-A/Ro and anti-SS-B/La IgG antibodies | Serum (1 ml) | Immuno Blot |
| Anti-Ro/SSA IgG antibodies | Serum (1 ml) | Immuno Blot |
| Anti-SSB/La IgG antibodies | Serum (1 ml) | Immuno Blot |
| Tacrolimus | Serum (3 ml) | Chemiluminescent Immunoassay (CMIA) |
| Tissue Transglutaminase IgA (tTG-IgA) Test | Serum (2 ml) | Enzyme Immunoassay |
| Total Prostate-Specific Antigen (PSA) Test | Serum (1 ml) | CLIA |
| TSH receptor antibodies (TRAB) | Serum (1 ml) | CLIA |
| Tissue Transglutaminase (TTG)/Deamidated Gliadin Peptide (DGP)(IgA+IgG) Test | Serum (2 ml) | Enzyme Immunoassay |
| Tumour Necrosis Factor alpha (TNF alpha) | Serum (1-2 ml) | Enzyme Immunoassay |
| Urine 24 hours for VMA (Vanillylmandelic acid) | 24 hours of Urine (10 ml) | HPLC |
Keynotes on Immunology Laboratory
- The laboratory collaborates with the Immune Monitoring Core Facility in the evaluation of immunodeficiency, whether congenital or acquired through transplantation, especially in clinical trials. The laboratory is in charge of testing that is on the verge of becoming clinical — tests created there frequently make their way into full clinical trials.
- IFA Stands for Indirect Fluorescent Antibody.
- The calprotectin stool test is noninvasive and it is tested to determine if you have an inflammatory or noninflammatory bowel condition. Colonoscopy or sigmoidoscopy is the alternative assay of the calprotectin stool test.
- The most common specimen container for immunology assay is a red-top tube or gel-barrier tube.
- ENA-6S-Profile is an ELISA test system for the qualitative determination of IgG antibodies against six extractable nuclear antigens (ENA), SS-A (Ro 52, Ro 60) SS-B, Sm, RNP/Sm, Scl-70 and Jo-1 in human serum or plasma that helps to the diagnosis of inflammatory rheumatic connective tissue diseases.
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