Introduction of Microbiology and Serology Laboratory
Table of Contents
Here we are discussing not general microbiology but medical/clinical microbiology it is a discipline that encloses a wide range of testing methodologies, and it is complex in terms of organisms (bacteria, viruses, fungi, parasites) and methods used to isolate and identify them. Although significant improvements in testing methodologies have been made, clinical microbiology remains heavily reliant on culture-based methods and phenotypic techniques for the identification of culture microbes. The wide variety of etiological agents/pathogens and testing methods that are available makes microbiological testing challenging, and therefore error detection and correction are important components of quality microbiology laboratory testing.
While the serology laboratory analyzes blood specimens for diseases of public health significance. A serology blood test is done to detect and measure the levels of antibodies as a result of exposure to a particular pathogen/microbe (bacteria or virus).
Microbiology and Serology Laboratory List of Tests, Specimen, and Testing Method
| Name of Test | Specimen | Testing Method |
| Acid-Fast bacilli(AFB) Smear Microscopy (2 samples) | Sputum (3-5 ml) | Microscopy |
| Anti HCV Antibody Rapid/ Quick/Spot Test | Serum (1-2 ml) | Immunochromatogrphy |
| Blood Culture and Sensitivity | Whole Blood (5 ml) | Microbiology |
| Bordetella pertussis IgG | Serum (1-2 ml) | Enzyme Immunoassay (EIA) |
| Buccal smear | Swab ( 1 gm) | Flocculation method |
| CHICKEN POX Varicella-Zoster Virus (VZV) IgG Antibodies | Serum (1-2 ml) | ELISA |
| CHICKEN POX Varicella-Zoster Virus (VZV) IgM Antibodies | Serum (1-2 ml) | ELISA |
| Chlamydia trachomatis Antibody Testing | Serum (1-2 ml) | ELISA |
| Cryptococcus Antigen – Blood and CSF | Serum/CSF (1-2 ml) | Immunochromatography |
| Cytomegalovirus (CMV)IgG Antibodies | Serum (1 ml) | ELISA |
| Cytomegalovirus (CMV) IgG & IgM Antibodies | Serum (1 ml) | ELISA |
| Cytomegalovirus (CMV)IgM Antibodies | Serum (1 ml) | ELISA |
| Dengue Fever Virus Antibodies, IgG & IgM (Quantitative) | Serum (1 ml) | ELISA |
| Echinococcus Antibody, IgG | Serum (1 ml) | EIA |
| Epstein-Barr Virus (EBV) IgM and IgG antibodies Tests | Serum (1 ml) | ELISA |
| HBsAg Rapid/Quick/spot Test | Serum (1 ml) | Immunochromatography |
| H. Pylori Antigen Test | Serum (1 ml) | Immunochromatography/ ELISA |
| Helicobacter pylori Antibodies, IgA | Serum (1 ml) | ELISA |
| Helicobacter pylori Antibodies, IgG | Serum (1 ml) | ELISA |
| Hepatitis A Virus (HAV)IgG & IgM Antibodies | Serum (1 ml) | CLIA |
| Hepatitis A Virus (HAV) IgM Antibodies | Serum (1 ml) | CLIA |
| Hepatitis B Core (HBC) Total Antibodies | Serum (1-2 ml) | CLIA |
| Hepatitis B Surface Antigen (HBsAg) Neutralization | Serum (1-2 ml) | CLIA |
| Hepatitis B Surface Antigen (HBsAg) Quantitative | Serum (1-2 ml) | CLIA |
| Hepatitis B e-Antibody (HBeAb or Anti-HBe) Assay | Serum (2-3 ml) | CLIA |
| Hepatitis B Envelope Antigen (HBeAg) Assay | Serum (2-3 ml) | CLIA |
| Hepatitis B Core IgM antibody (anti-HBcIgM) Assay | Serum (1-2 ml) | CMIA |
| Hepatitis Be Antigen and Antibody (HBeAg and HBeAb)Evaluation | Serum (1-2 ml) | CMIA |
| Hepatitis C Antibody/( Anti-HCV) antibodies | Serum (1-2 ml) | CLIA |
| Hepatitis Delta Virus (HDV) IgG Antibodies | Serum (1-2 ml) | CLIA |
| Hepatitis E Virus (HEV) IgG Antibodies | Serum (1-2 ml) | CLIA |
| Hepatitis E Virus (HEV) IgM Antibodies | Serum (1-2 ml) | EIA |
| Herpes Simplex Virus Type 1 and/or 2 Antibodies, IgG & IgM | Serum (2-3 ml) | EIA |
| HIV – DUO p24 Antigen & Antibody to HIV1 & 2 | Serum (1-2 ml) | CLIA |
| HIV-1 RNA PCR, Quantitative | Blood (2-3 ml) in EDTA Tube (lavender-top) | Real-Time PCR |
| HIV-1/HIV-2 Rapid Screen/Quick/Spot | Serum (1-2 ml) | Immunochromatography |
| Recombinant K39 antigen for visceral leishmaniasis/ Indian kala-azar | Serum (1-2 ml) | Immunochromatography |
| Potassium hydroxide (KOH)Mount for Fungus | Suspected specimen (nail, hair, skin scrapping) | Microscopy |
| Mantoux test for Tuberculosis | Tuberculin vial (outside for injection)-0.1 ml of a liquid containing 5 TU | Intradermal test for Hypersensitivity |
| Malarial Antibody | Serum (1-2 ml) | Immunochromatography |
| Malaria parasite detection in blood smear | Blood (2-3 ml) in EDTA Tube (lavender-top) | Microscopy |
| Typhoid IgG/IgM Combo Rapid Test | Serum (1-2 ml) | Immunochromatography |
| Rubella Antibody, IgG | Serum (1-2 ml) | ELISA |
| Rubella Antibody, IgM | Serum (1-2 ml) | ELISA |
| Detection of group B streptococcal antigen | Serum (1-2 ml) | Latex Particle Agglutination |
| Syphilis Antibody | Serum (1-2 ml) | ELISA |
| Toxoplasma Antibody, IgG | Serum (1-2 ml) | ELISA |
| Toxoplasma Antibody, IgM | Serum (1-2 ml) | ELISA |
| TPHA | Serum (1-2 ml) | Immunoblotting |
Keynotes on Microbiology and Serology Laboratory
- ELISA stands for enzyme-linked immunoassay.
- CLIA stands for Chemiluminescence immunoassay.
- Microbiology is the branch of science that deals with microscopic organisms/microbes (bacteria, viruses, fungi, and parasites) and their interaction with other microscopic and macroscopic organisms.
- Serology has been used historically to confirm infections with microbes like bacteria, fungi, viruses, and parasites that are difficult to detect by other methods.
- Prions are abnormal, pathogenic agents that are transmissible and are able to induce abnormal folding of specific normal cellular proteins human prion diseases are Creutzfeldt-Jakob disease (CJD), Fatal Familial Insomnia (FFI), and Kuru.
I absolutely love your blog and find almost all of your post’s to be what precisely I’m looking for. Would you offer guest writers to write content available for you? I wouldn’t mind creating a post or elaborating on some of the subjects you write concerning here. Again, awesome weblog!
Hey there! I could have sworn I’ve been to this website before but after reading through some of the post I realized it’s new to me. Anyhow, I’m definitely glad I found it and I’ll be bookmarking and checking back frequently!
I really appreciate your work, Great post.
Some really interesting information, well written and broadly user pleasant.
Keep working ,fantastic job!
I like what you guys are up too. Such intelligent work and reporting! Carry on the superb works guys I have incorporated you guys to my blogroll. I think it’ll improve the value of my web site :).
What i don’t understood is in truth how you’re no longer actually a lot more smartly-liked than you might be right now. You are very intelligent. You know thus significantly in terms of this subject, produced me individually believe it from so many numerous angles. Its like men and women don’t seem to be involved until it¦s something to do with Lady gaga! Your personal stuffs nice. At all times handle it up!
Hey very nice blog!! Man .. Excellent .. Amazing .. I’ll bookmark your web site and take the feeds also…I am happy to find so many useful info here in the post, we need work out more techniques in this regard, thanks for sharing. . . . . .
It¦s really a great and useful piece of information. I am glad that you shared this useful information with us. Please keep us informed like this. Thanks for sharing.
Only wanna admit that this is very helpful, Thanks for taking your time to write this.
I truly enjoy studying on this site, it has wonderful articles.
Good write-up, I am normal visitor of one’s website, maintain up the nice operate, and It’s going to be a regular visitor for a long time.
Woh I like your articles, saved to my bookmarks! .
Im obliged for the article post. Cool.
I’ve been surfing online more than 3 hours these days, yet I never found any attention-grabbing article like yours. It’s pretty worth sufficient for me. In my view, if all webmasters and bloggers made good content material as you probably did, the net will probably be a lot more useful than ever before.
I don’t commonly comment but I gotta admit thankyou for the post on this one : D.
What i don’t realize is if truth be told how you’re not actually a lot more smartly-favored than you may be right now. You’re very intelligent. You already know thus considerably in the case of this topic, produced me individually consider it from numerous various angles. Its like women and men don’t seem to be involved unless it is something to do with Girl gaga! Your own stuffs nice. At all times take care of it up!