Incubator: Introduction, Principle, Handling Procedure, Types, Uses, and Keynotes

Blood agar and chocolate agar incubated CO2 Incubator

Introduction of Incubator The incubator is an electrical device in which the incubation process is performed which conditions an enclosure to a predetermined temperature since it provides and maintains all artificial optimal conditions for the growth of microbial culture as well as cell and tissue …

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Brain heart infusion (BHI) Broth: Introduction, Composition, Principle, Preparation, Test Procedure, Result-Interpretation, Modifications, Uses, and Keynotes

Blood inoculated BHI broth after 4 days of incubation showing bacterial growth

Introduction of BHI Broth Brain Heart Infusion (BHI) broth is a highly nutritive medium and is thus useful for cultivating a wide variety of microorganisms like aerobic and anaerobic bacteria, yeasts, and molds.  BHI broth is commonly used in blood culture work and allied pathological …

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RPR Test: Introduction, Principle, Procedure, Result-Interpretation, Clinical Significance, and Keynotes

RPR Test: Introduction, Principle, Procedure, Result-Interpretation, Clinical Significance, and Keynotes

Introduction of Rapid Plasma Reagin (RPR) Test RPR is a screening non-specific test for syphilis caused by a bacterium, Treponema pallidum. It has replaced the VDRL test due to having properties like no need of preparing fresh reagents daily, no need for a microscope, and …

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TPHA Test: Introduction, Principle, Procedure, Result-Interpretation, Clinical Significance, and Keynotes

TPHA Test: Introduction, Principle, Procedure, Result-Interpretation, Clinical Significance, and Keynotes

Introduction of TPHA Test TPHA test  stands for Treponema pallidum hemagglutination. This is a confirmatory test for syphilis that is caused by a bacterium, T. pallidum. TPHA test is a sensitive indirect hemagglutination test, that detects specific Treponema pallidum antibodies in serum within one hour. It uses …

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VDRL Test: Introduction, Principle, Procedure, Result-Interpretation,Limitations, and Keynotes

VDRL Test Introduction,Principle, Procedure, Result-Interpretation,Limitations, and Keynotes

Introduction of VDRL Test VDRL stands for Veneral disease research laboratory. The laboratory was renamed the Treponemal Pathogenesis and Immunology Branch of the United States Public Health Service. The Venereal Disease Research Laboratory (VDRL) test is one of two variations of micro flocculation procedures used for serological testing …

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Staphylococcus aureus: Introduction, Pathogenicity, Lab Diagnosis, Treatment, and Keynotes

Staphylococcus aureus: Introduction, Pathogenicity, Lab Diagnosis, Treatment, and Keynotes

Introduction of Staphylococcus aureus Scientific classification of Staphylococcus aureus is given as: Domain Bacteria Phylum Firmicutes Class Bacilli Order Bacillales Family Staphylococceae Genus Staphylococcus Species aureus Table: Biological Classification of Staphylococcus aureus Staphyle means bunch and Kokko mean berry. Staphylococcus aureus was isolated by Pasteur (1880) from a pus sample. …

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CLED Agar: Introduction, Composition, Principle, Preparation, Testing Procedure, Colony Characteristics, Modifications, Limitations, and Keynotes

Staphylococcus aureus (11 o'clock) Klebsiella pneumoniae (5 o'clock) and Pseudomonas aeruginosa (8 o'clock) growth on CLED agar-Back View

Introduction of CLED Agar CLED stands for cysteine, lactose, and electrolyte-deficient. CLED agar or medium is a differential culture medium used for the isolation and enumeration of bacteria in urine specimens from suspected cases of urinary tract infection (UTI). It favors the growth of all …

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Lowenstein-Jensen (LJ) Medium: Introduction, Principle Composition, Preparation, Procedure, Result- Interpretation, Limitation, and Keynotes

Lowenstein-Jensen (LJ) Medium: Introduction, Principle Composition, Preparation, Procedure, Result- Interpretation, Limitation, and Keynotes

Introduction of Lowenstein-Jensen (LJ) Medium Lowenstein-Jensen (LJ) Medium is named after the surnames of  Austrian pathologist Ernst Lowenstein (1878–1950) and the Danish chemist Kai Arne Jensen (1908–1992). The etiological agent of tuberculosis is Mycobacterium tuberculosis. It requires aerobic conditions and a protein-enriched medium for culture. The short …

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