Lactose Fermenter (LF) and Non-Lactose Fermenter (NLF) Gram-Negative Bacteria: Introduction, List of LF and NLF Bacteria, and Keynotes

Lactose Fermenter (LF) and Non-Lactose Fermenter (NLF) Gram-Negative Bacteria: Introduction, List of LF and NLF Bacteria, and Keynotes
Fig. Lactose Fermenter (LF)-Pink colony-Right side and Non-Lactose Fermenter (NLF)-pale or no color change-Left side Gram-Negative Bacteria on MacConkey agar

Introduction of LF and NLF Gram-Negative Bacteria on MacConkey Medium

MacConkey agar/ medium is recommended for use as a selective, differential, and indicator growth medium for the isolation of gram-negative bacilli including coliform organisms and enteric pathogens, on the basis of lactose fermentation. Peptones (meat and casein) and the pancreatic digest of gelatin provide the essential nutrients, vitamins, and nitrogenous factors required for the growth of microbes. Lactose monohydrate is the fermentable source of carbohydrates. The selective action of this medium is contributed by crystal violet and bile salts, which are inhibitory to most species of gram-positive bacteria. Sodium chloride maintains the osmotic balance in the medium whereas neutral red is a pH indicator that turns red at a pH below 6.8 (lactose fermenter) and is colorless at any pH greater than 6.8 (non-lactose fermenter). Agar is the solidifying agent.

List of LF Bacteria and NLF Bacteria

The list of lactose fermenter Gram-negative bacteria on the basis of MacConkey medium are as follows-

S. No.OrganismsColony Morphology
1Escherichia coliLactose fermenter
2Klebsiella spp.Lactose fermenter
3Citrobacter spp.Late lactose fermenter
4Enterobacter spp.Lactose fermenter
5Serratia spp.Late lactose fermenter
6Erwinia spp.Lactose fermenter
Table: Lactose fermenter Gram-negative bacteria

The list of non-lactose fermenter Gram-negative bacteria on the basis of MacConkey medium are as follow-

S. No.OrganismsColony Morphology
1Salmonella spp.Non-Lactose Fermenter
2Shigella spp. Non-Lactose Fermenter
except S.sonnei(Late lactose fermenter)
3Proteus spp.Non-Lactose Fermenter
4Providencia spp.Non-Lactose Fermenter
5MorgenellaNon-Lactose Fermenter
6Hafnia alveiNon-Lactose Fermenter
7 E. tarda Non-Lactose Fermenter
8Pseudomonas spp.Non-Lactose Fermenter
9Acinetobacter spp.Non-Lactose Fermenter
10Burkholderia cepacia complexNon-Lactose Fermenter
Table: Non-lactose fermenter Gram-negative bacteria

Keynotes on Lactose Fermenter (LF) and Non-Lactose Fermenter (NLF) Gram-Negative Bacteria

  1. Pasteurella species (including Pasteurella multocida) will not grow on MacConkey Agar.
  2. MacConkey agar without bile salt- It uses both gram-negative and gram-positive bacteria
  3. MacConkey agar with bile salt- Selective for gram-negative bacteria but Enterococcus species may grow.
  4. MacConkey agar with bole sat and crystal violet: Strict selective medium for gram-negative bacteria that also inhibits Entercoccus species due to having crystal violet in its composition.
  5. MacConkey Agar with Sorbitol (Sorbitol-MacConkey agar) is to be used as a selective and differential medium for the detection of enterohemorrhagic Escherichia coli O157:H7.
  6. Fermenter,oxidizer and non-utilizer bacteria can only be confirmed using oxidative fermentative (OF) test.

LF and NLF Gram-Negative Bacteria

Escherichia coli colony morphology on MacConkey agar

Escherichia coli colony morphology on MacConkey agar
Fig. Escherichia coli colony morphology on MacConkey agar showing pink colonies

LF and NLF Colonies of Klebsiella and Pseudomonas on MacConkey medium

LF and NLF Colonies of Klebsiella and Pseudomonas on MacConkey medium
Fig. LF (pink) and NLF (pale) Colonies of Klebsiella and Pseudomonas on MacConkey medium

Mucoid lactose fermenter (MLF) colony of Klebsiella pneumoniae on MacConkey agar

Mucoid lactose fermenter (MLF) colony of Klebsiella pneumoniae on MacConkey agar
Fig. Mucoid lactose fermenter (MLF) colony of Klebsiella pneumoniae on MacConkey agar

Dangerous mucoid lactose fermenter (MLF) colony of Klebsiella pneumoniae on MacConkey agar

Dangerous mucoid lactose fermenter (MLF) colony of Klebsiella pneumoniae on MacConkey agar
Fig. Dangerous mucoid lactose fermenter (MLF) colony of Klebsiella pneumoniae on MacConkey agar

Proteus mirabilis colony characteristics on Macconkey medium showing non-lactose fermenter (NLF) colonies

Proteus mirabilis colony characteristics on Macconkey medium showing non-lactose fermenter (NLF) colonies
Fig. Proteus mirabilis colony characteristics on MacConkey medium showing non-lactose fermenter (NLF) colonies

Salmonella growth on MacConkey agar

Salmonella growth on MacConkey agar
Fig. Salmonella growth on MacConkey agar

Shigella colony morphology on MacConkey agar

Shigella colony morphology on MacConkey agar
Fig. Shigella colony morphology on MacConkey agar

Proteus vulgaris growth on MacConkey agar

Proteus vulgaris growth on MacConkey agar
Fig. Proteus vulgaris growth on MacConkey agar

Pseudomonas aeruginosa colony morphology on MacConkey agar

Pseudomonas aeruginosa colony morphology on MacConkey agar
Fig. Pseudomonas aeruginosa colony morphology on MacConkey agar

Mucoid strain of Pseudomonas aeruginosa colony morphology on MacConkey agar

Mucoid strain of Pseudomonas aeruginosa colony morphology on MacConkey agar
Fig. The mucoid strain of Pseudomonas aeruginosa colony morphology on MacConkey agar

Acinetobacter colony morphology on MacConkey agar

Acinetobacter colony morphology on MacConkey agar
Fig. Acinetobacter colony morphology on MacConkey agar

Serratia marcescens growth on Muller-Hinton agar with pigment expression Demonstration

Serratia marcescens growth on Muller-Hinton agar with pigment expression Demonstration
Fig. Serratia marcescens growth on Muller-Hinton agar with pigment expression (incubation at room temperature) Demonstration

Aeromonas hydrophila colony morphology on MacConkey agar

Aeromonas hydrophila colony morphology on MacConkey agar
Fig. Aeromonas hydrophila colony morphology on MacConkey agar

Alcaligenes faecalis growth on MacConkey agar

Alcaligenes faecalis growth on MacConkey agar
Fig. Alcaligenes faecalis growth on MacConkey agar

Further Reading

  • https://www.sciencedirect.com/topics/medicine-and-dentistry/providencia
  • http://www.oxoid.com/uk/blue/prod_detail/prod_detail.asp?pr=CM0007
  • https://universe84a.com/collection/acinetobacter-2/
  • https://www.sciencedirect.com/topics/medicine-and-dentistry/edwardsiella
  • https://universe84a.com/macconkey-agar-introduction/
  • https://universe84a.com/oxidative-fermentative-test/
  • Cowan & Steel’s Manual for identification of Medical Bacteria. Editors: G.I. Barron & R.K. Felthani, 3rd ed 1993, Publisher Cambridge University Press.
  • Bailey & Scott’s Diagnostic Microbiology. Editors: Bettey A. Forbes, Daniel F. Sahm & Alice S. Weissfeld, 12th ed 2007, Publisher Elsevier.
  • Clinical Microbiology Procedure Handbook, Chief in editor H.D. Isenberg, Albert Einstein College of Medicine, New York, Publisher ASM (American Society for Microbiology), Washington DC.
  • https://catalog.hardydiagnostics.com/cp_prod/content/hugo/ofbasalmedium.htm
  • Colour Atlas and Textbook of Diagnostic Microbiology. Editors: Koneman E.W., Allen D.D., Dowell V.R. Jr, and Sommers H.M.
  • Jawetz, Melnick and Adelberg’s Medical Microbiology. Editors: Geo. F. Brook, Janet S. Butel & Stephen A. Morse, 21st ed 1998, Publisher Appleton & Lance, Co Stamford Connecticut.
  • Mackie and Mc Cartney Practical Medical Microbiology. Editors: J.G. Colle, A.G. Fraser, B.P. Marmion, A. Simmous, 4th ed, Publisher Churchill Living Stone, New York, Melborne, Sans Franscisco 1996.
  •  Textbook of Diagnostic Microbiology. Editors: Connie R. Mahon, Donald G. Lehman & George Manuselis, 3rd edition2007, Publisher Elsevier.

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    日本女孩稱為清涼綠茶
    俄羅斯女孩被稱為金酥麻茶
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    喝茶術語就像是進入茶道的第一步,就像是蓋房子打地基一樣。在這裡,我們將這些外送茶入門術語分類,讓大家能夠清楚地理解,讓喝茶變得更加容易上手。

    魚:指的自行接客的小姐,不屬於任何茶莊。
    茶:就是指「小姐」的意思,由茶莊安排接客。
    定點茶:指由茶莊提供地點,客人再前往指定地點與小姐交易。
    外送茶:指的是到小姐到客人指定地點接客。
    個工:指的是有專屬工作室自己接客的小姐。
    GTO:指雞頭也就是飯店大姊三七茶莊的意思。
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    內機:盤商應召站提供茶園的人。
    經紀人:幫內機找美眉的人。
    馬伕:外送茶司機又稱教練。
    代調:收取固定代調費用的人(只針對同業)。
    阿六茶:中國籍女子,賣春的大陸妹。
    熱茶、熟茶:年齡比較大、年長、熟女級賣春者(或稱阿姨)。
    燙口 / 高溫茶:賣春者年齡過高。
    台茶:從事此職業的台灣小姐。
    本妹:從事此職業的日本籍小姐。
    金絲貓:西方國家的小姐(歐美的、金髮碧眼的那種)。
    青茶、青魚:20 歲以下的賣春者。
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    龍、小叮噹、小叮鈴:體型比較肥、胖、臃腫、大隻的小姐。

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