Introduction
Table of Contents
LAMP stands for Loop-mediated Isothermal Amplification while PCR full form is polymerase chain reaction and LAMP is the nucleic acid amplification assay. The lamp is a newer technique whereas PCR is older. The LAMP is comparatively more efficient and cost-effective than PCR testing.
Differences between LAMP and PCR Assays
Differences between them are as follows-
| LAMP | PCR |
| Emerging Method | Older method |
| Exponential accumulation of additional dsDNA is faster. | Slower |
| No need for multiple temperature cycles | Multiple temperature cycles are needed. |
| LAMP amplification also depends on the low range of heat at around 60-65ºC. | A higher range of heat is required i.e. 56-95ºC. |
| Result within minutes (average 30 minutes) | Result within hours (average 4 hours) |
| It can ve handled in a simple setup. | It needs an advanced level of setup. |
| Low manpower and basic training are sufficient. | High manpower and highly skilled laboratory personnel are mandatory. |
| Equipment name-MicrosensDx RapiPRO™ Loop-mediated Isothermal Amplification (LAMP) SARS-CoV-2 test | BioRad CFX-96, Applied Biosystem-Qunnstudio 5, RotorGene Q |
| It has far greater accuracy and sensitivity than antigen testing for SARS-CoV-2. | It has too. |
| More efficient and cost-effective | Costly |
| RapiPRO™ LAMP for SARS-CoV-2 test has widely applied on large industrial sites and in airports. | It is hard to install in such areas. |
| No need for expensive reagents or instruments | It needs expensive reagents or instruments. |
| Highly sensitive (nearly 100-fold more sensitive than conventional RT-PCR methods). | Comparatively less sensitive |
Keynotes
- Loop-mediated isothermal amplification (LAMP) assay is a rapid diagnostic for COVID-19.
- RT-PCR for COVID-19 is a gold standard assay.
- Lamp is a nucleic acid amplifixation assay and higly senstive than conventional RT-PCR methods.
- PCR is a nucleic acid targets assay i.e. N2 (nucleocapsid), E (envelope), S (spike protein),etc.
Further Readings
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182526/
- https://www.microsensdx.com/lamp-vs-pcr-choosing-the-appropriate-sars-cov-2-covid-19-diagnostic-
- https://universe84a.com/sars-cov-2-genes-detection/
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