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Introduction of Haemophilus The genus Haemophilus is a pleomorphic Gram-negative coccobacilli fastidious bacteria and thus they do not grow on an ordinary medium like nutrient agar and they need chocolate agar to grow (H. influenzae) due to having X, V, and both X and V growth factors in it. …
Introduction of Staphylococcus and Micrococcus Staphylococcus (pleural-staphylococci) is spherical, non-motile, gram-positive in singles, pairs, and clusters. On nutrient agar, growth is opaque and golden yellow or white color. Catalase and coagulase test positive (Staphylococcus aureus), oxidase negative, aerobic or facultative anaerobe. Parasite of humans and …
Introduction of Streptococcus pneumoniae and Viridans streptococci Streptococcus pneumoniae is also called pneumococcus and pneumococcus is the commensals of the upper respiratory tract. It is an important etiological agent of pneumonia and otitis media in children that differs from streptococci in its morphology (diplococci), bile …
Introduction of String Test String test is a special test of bacteria to identify Vibrio cholerae (String test positive) from Vibrio species and other bacteria like Aeromonas species and Plesiomonas shigelloides. This assay can perform on a glass slide or plastic petri dish, suspending overnight …
Introduction of Gram-Positive Bacteria Identification Identification of Gram-positive bacteria is a little bit harder than Gram-negative bacteria since the most common bacterial etiological agents are Gram-negative bacteria and the vendors or suppliers are directly involved in supplying common tests reagents and test kit accessories. Due …
Triple Sugar Iron Agar Test The triple Sugar Iron (TSI) Agar test is a combo test and is useful for the identification of mainly gram-negative bacteria, on the basis of the following tests- Composition of Triple Sugar Iron Agar Triple Sugar Iron Agar (TSI) (Oxoid, England) …
Introduction Satellitism test or satellite test is applied to identify Haemophilus influenzae. It is a fastidious bacterium and thus it needs extra ingredients like hemin (factor X) and NAD (factor V) to grow. It uses the X factor to produce essential respiratory enzymes such as cytochromes, catalases, …
Introduction SIM stands for sulfide, Indole, and Motility and SIM medium is applied for the differentiation of gram-negative enteric bacilli. SIM test assists to isolate the bacteria on the basis of sulfide production, indole formation, and motility and thus SIM test is a combination of three …