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Introduction The urease test is also known as the urea hydrolyzation test or urea test and it is important for certain bacteria and fungi that possess this enzyme which hydrolyzes urea releasing ammonia into the medium. This produces a change in the pH of the …
Introduction of Coagulase Test The coagulase test is an enzymatic assay that is applied to differentiate Staphylococcus aureus (positive) from coagulase-negative staphylococci (CoNS). Coagulase is an enzyme formed by S. aureus which changes soluble fibrinogen of plasma to insoluble fibrin. S. aureus produces two forms of coagulase. One is bound coagulase …
Introduction A citrate utilization test is the most commonly used routine biochemical test for Gram-negative rods in bacteriology for the identification of bacteria on the basis of citrate utilization. Klebsiella pneumoniae is the citrate utilization-positive organism while Escherichia coli is negative. Principle of Citrate Utilization Test …
Introduction of Catalase Test The catalase test is the most common in bacteriology and is commonly used to differentiate those bacteria that produce this enzyme, such as staphylococci, from non-catalase producing bacteria such as streptococci. It is two types depending on the testing methods and they …
Introduction of Oxidase Test (Kovac’s Method) Gordon and McLeod introduced the application of a dimethyl-phenylenediamine dihydrochloride solution to test for the presence of oxidase systems in 1928. In particular, they used the test to differentiate N. gonorrhoeae (oxidase test positive) from Staphylococcus species and Streptococcus species (oxidase negative). The sensitivity of the …
Introduction Courses offered in Medical Laboratory Technology are Medical Laboratory Assistant (LA), Certificate in Medical Laboratory Technology (CMLT), Bachelor of Science in Medical Laboratory Technology (B.Sc. MLT), and Master of Science in Medical Lab Technology (M.Sc. MLT) but this course has further specialized into other …
Introduction The common instruments used in the microbiology laboratory include a bunch of different types of instruments required for a lot of various processes conducted within the laboratory. e.g. Bunsen burner, incubator, autoclave, hot air oven, analytical balance, densitometer, centrifuge, microscope, colony counter, etc. Analytical …