Introduction of Proteus mirabilis
Table of Contents
The genus Proteus was discovered in 1885 by Hauser and it is also named after a Greek god. Proteus is a member of the family, Enterobacteriaceae and it is a Gram-negative, oxidase-negative, fimbriated, motile, non-sporing rod-shaped bacterium without capsule and having a size of 0.4–0.8 μm in diameter and 1.0–3.0 μm in length. Proteus mirabilis is naturally found in the natural environment and also the intestinal tract. It is a gut bacterium inside our intestines whereas, outside the gut, it can cause serious infections. It is an etiological agent of catheter-associated UTI (CAUTI), sepsis, and septic shock if the infection goes up and causes cystitis and pyelonephritis.
Biochemical Reactions of Proteus mirabilis
| Basic Features | Properties |
| 1. Gram Staining | Gram-Negative Rods (GNRs) |
| 2. Spore | Non-Sporing |
| 3. Capsule | Negative |
| 4. Motility | Motile |
| 5. Pigment | Negative |
| 6. Growth in potassium cyanide (KCN) medium | Positive |
| 7. Catalase test | Positive |
| 8. Oxidase test | Negative |
| 9. Nitrate reduction test | Positive |
| 10. MR (Methyl Red) test | Positive |
| 11. VP (Voges- Proskauer) assay | Negative |
| 12. OF (Oxidative-Fermentative) test | Fermentative (facultative anaerobes) |
| 13. Gas from Glucose | Positive |
| 14. H2S production | Positive |
| 15. Indole formation | Negative |
| 16. Urease/ urea hydrolysis test | Positive |
| 17. Citrate/citrate utilization | Positive |
| 18. DNase test | Variable |
| 19. Glucose fermentation | Positive |
| 20. Maltose fermentation | Negative |
| 21. Lactose fermentation | Negative |
| 22. Sucrose fermentation | Negative |
| 23. Xylose fermentation | Positive |
| 24. Mannitol fermentation | Negative |
| 25. Acetate Utilization | Negative |
| 26. ONPG (β-galactosidase) | Negative |
| 27. Phenylalanine Deaminase (PDA)/PPA Test | Positive |
| 28. Lipase test | Positive |
| 29. Esculin Hydrolysis test | Negative |
| 30. Lysine Decarboxylase Test | Negative |
| 31. Ornithine Decarboxylase Test | Positive |
| 32. Arginine Dihydrolase Test | Negative |
| 33. Gelatin Hydrolysis | Positive |
| 34. Tryptophan Deaminase | Negative |
| 35. Casein Hydrolysis | Negative |
Keynotes on Proteus
- In Greek mythology, Proteus means sea-god.
- Every year about 150 million people are affected by Proteus mirabilis globally.
- It is the bacterium of concern since in the USA, it accounts for about 3% of all hospital infections and 44% of CAUTI.
- The principal virulence factors associated with infection are flagella, pili, urease, hemolysin, and metal intake.
- Multiple drug-resistant (MDR) strains to carry R plasmids have become very important in nosocomial infections.
- The distinctive characters of the genus are PPA, urease, and H2S positive.
- Indole helps to differentiate P. vulgaris ( positive) from P. mirabilis ( negative).
- Dienes phenomenon or typing is using successfully to determine the relationship between strains of Proteus species in studies of cross-infection.
- Swarming growth of Proteus contains swimmer and swarmer cells and these cells can be determined using Gram’s staining i.e. swimmer cells- small-near the center of the growth plate while swarmer cells- large-away the center of growth plate as shown in footages.
- Swarming growth of Proteus inhibited by following agents-
- Agar (6%)
- Sodium azide (NaN3) (1:500)
- Chloral hydrate (1:500)
- Boric acid (1:1000)
- Alcohol (5-6%)
Proteus Footages
Proteus mirabilis non-lactose fementing colony on MacConkey agar
Proteus mirabilis in Gram staining of culture showing Gram-negative small to large rods or bacilli
Proteus mirabilis biochemical reactions in TSI, MIU and citrate agar
Antibiotic Sensitivity Testing (AST) Pattern of Proteus mirabilis
Swarming growth of Proteus on blood agar
Dienes Phenomenon of Proteus mirabilis with different strains
Swarming growth of Proteus inhibiting agents
Proteus made ‘Natural Bacterial Agar Art’
Further Reading
- https://jb.asm.org/content/195/6/1305
- https://www.karger.com/Article/FullText/514097
- https://www.annualreviews.org/doi/pdf/10.1146/annurev.mi.32.100178.000533
- https://universe84a.com/proteus-general-characteristics
- Williams FD, Schwarzhoff RH. 1978. Nature of the swarming phenomenon in Proteus. Annu. Rev. Microbiol. 32:101–122.
- https://www.sciencedirect.com/topics/medicine-and-dentistry/proteus-mirabilis
- Armbruster CE, Mobley HLT. 2012. Merging mythology and morphology: the multifaceted lifestyle of Proteus mirabilis. Nat. Rev. Microbiol. 10:743–754
- Morgenstein RM, Szostek B, Rather PN. 2010. Regulation of gene expression during swarmer cell differentiation in Proteus mirabilis. FEMS Microbiol. Rev. 34:753–763.
- Rather PN. 2005. Swarmer cell differentiation in Proteus mirabilis. Environ. Microbiol. 7:1065–1073.
- Bailey & Scott’s Diagnostic Microbiology. Editors: Bettey A. Forbes, Daniel F. Sahm & Alice S. Weissfeld, 12th ed 2007, Publisher Elsevier.
- Clinical Microbiology Procedure Handbook, Chief in editor H.D. Isenberg, Albert Einstein College of Medicine, New York, Publisher ASM (American Society for Microbiology), Washington DC.
- Colour Atlas and Textbook of Diagnostic Microbiology. Editors: Koneman E.W., Allen D.D., Dowell V.R. Jr, and Sommers H.M.
- Textbook of Diagnostic Microbiology. Editors: Connie R. Mahon, Donald G. Lehman & George Manuselis, 3rd edition 2007, Publisher Elsevier
- Jawetz, Melnick and Adelberg’s Medical Microbiology. Editors: Geo. F. Brook, Janet S. Butel & Stephen A. Morse, 21st ed 1998, Publisher Appleton & Lance, Co Stamford Connecticut.
- Mackie and Mc Cartney Practical Medical Microbiology. Editors: J.G. Colle, A.G. Fraser, B.P. Marmion, A. Simmous, 4th ed, Publisher Churchill Living Stone, New York, Melborne, Sans Franscisco 1996.
- Manual of Clinical Microbiology. Editors: P.R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover and R. H. Yolken, 7th ed 2005, Publisher ASM, USA
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Thanks for sharing your ideas on this blog. Also, a delusion regarding the banking institutions intentions when talking about foreclosures is that the traditional bank will not have my repayments. There is a certain quantity of time the bank will need payments every now and then. If you are far too deep inside hole, they will commonly call that you pay the particular payment fully. However, that doesn’t mean that they will not take any sort of installments at all. In case you and the lender can seem to work something out, the particular foreclosure procedure may end. However, when you continue to neglect payments under the new program, the home foreclosure process can just pick up where it was left off.
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2024娛樂城的創新趨勢
隨著2024年的到來，娛樂城業界正經歷著一場革命性的變遷。這一年，娛樂城不僅僅是賭博和娛樂的代名詞，更成為了科技創新和用戶體驗的集大成者。
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Understanding the processes and protocols within a Professional Tenure Committee (PTC) is crucial for faculty members. This Frequently Asked Questions (FAQ) guide aims to address common queries related to PTC procedures, voting, and membership.
1. Why should members of the PTC fill out vote justification forms explaining their votes?
Vote justification forms provide transparency in decision-making. Members articulate their reasoning, fostering a culture of openness and ensuring that decisions are well-founded and understood by the academic community.
2. How can absentee ballots be cast?
To accommodate absentee voting, PTCs may implement secure electronic methods or designated proxy voters. This ensures that faculty members who cannot physically attend meetings can still contribute to decision-making processes.
3. How will additional members of PTCs be elected in departments with fewer than four tenured faculty members?
In smaller departments, creative solutions like rotating roles or involving faculty from related disciplines can be explored. Flexibility in election procedures ensures representation even in departments with fewer tenured faculty members.
4. Can a faculty member on OCSA or FML serve on a PTC?
Faculty members involved in other committees like the Organization of Committee on Student Affairs (OCSA) or Family and Medical Leave (FML) can serve on a PTC, but potential conflicts of interest should be carefully considered and managed.
5. Can an abstention vote be cast at a PTC meeting?
Yes, PTC members have the option to abstain from voting if they feel unable to take a stance on a particular matter. This allows for ethical decision-making and prevents uninformed voting.
6. What constitutes a positive or negative vote in PTCs?
A positive vote typically indicates approval or agreement, while a negative vote signifies disapproval or disagreement. Clear definitions and guidelines within each PTC help members interpret and cast their votes accurately.
7. What constitutes a quorum in a PTC?
A quorum, the minimum number of members required for a valid meeting, is essential for decision-making. Specific rules about quorum size are usually outlined in the PTC’s governing documents.
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메인 서비스: 간편하고 효율적인 배송 및 구매 대행 서비스
1. 대행 서비스 주요 기능
메인 서비스는 고객이 한 번에 필요한 대행 서비스를 신청할 수 있도록 다양한 기능을 제공합니다.
배송대행 신청: 국내외 상품 배송을 대신 처리하며, 효율적인 시스템으로 신속한 배송을 보장합니다.
구매대행 신청: 원하는 상품을 대신 구매해주는 서비스로, 고객의 수고를 줄입니다.
엑셀 대량 등록: 대량 상품을 엑셀로 손쉽게 등록 가능하여 상업 고객의 편의성을 증대합니다.
재고 관리 신청: 창고 보관 및 재고 관리를 통해 물류 과정을 최적화합니다.
2. 고객 지원 시스템
메인 서비스는 사용자 친화적인 접근성을 제공합니다.
유저 가이드: 대행 서비스를 더욱 합리적으로 사용할 수 있도록 세부 안내서를 제공합니다.
운송장 조회: 일본 사가와 등 주요 운송사의 추적 시스템과 연동하여 운송 상황을 실시간으로 확인 가능합니다.
3. 비용 안내와 부가 서비스
비용 계산기: 예상되는 비용을 간편하게 계산해 예산 관리를 돕습니다.
부가 서비스: 교환 및 반품, 폐기 및 검역 지원 등 추가적인 편의 서비스를 제공합니다.
출항 스케줄 확인: 해외 배송의 경우 출항 일정을 사전에 확인 가능하여 배송 계획을 세울 수 있습니다.
4. 공지사항
기본 검수 공지
무료 검수 서비스로 고객의 부담을 줄이며, 보다 철저한 검수가 필요한 경우 유료 정밀 검수 서비스를 권장합니다.
수출허가서 발급 안내
항공과 해운 수출 건에 대한 허가서를 효율적으로 발급받는 방법을 상세히 안내하며, 고객의 요청에 따라 이메일로 전달됩니다.
노데이터 처리 안내
운송장 번호 없는 주문에 대한 새로운 처리 방안을 도입하여, 노데이터 발생 시 관리비가 부과되지만 서비스 품질을 개선합니다.
5. 고객과의 소통
카카오톡 상담: 실시간 상담을 통해 고객의 궁금증을 해결합니다.
공지사항 알림: 서비스 이용 중 필수 정보를 지속적으로 업데이트합니다.
메인 서비스는 고객 만족을 최우선으로 하며, 지속적인 개선과 세심한 관리를 통해 최상의 경험을 제공합니다.