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Introduction of API Test for Bacteria API test for bacteria as shown above picture is a 20-jumbo tests kit for a biochemical panel for the identification and differentiation of members of the family Enterobacteriaceae. API stands for Analytical Profile Index. It is an API 20E Test kit …
Introduction of Bile Solubility Test Bile solubility test uses for presumptive identification of Streptococcus pneumoniae. Principle of Bile Solubility Test Bile salts, specifically sodium deoxycholate and sodium taurocholate, have the capacity to selectively lyse Streptococcus pneumoniae when added to actively growing bacteria in agar or broth media. S. pneumoniae produces autolytic …
Introduction of Malonate Test Malonate test uses to check the ability of the organism to utilize malonate as the sole source of carbon for energy. To differentiate bacteria on the basis of a malonate utilization test. It is a colorful test to check the ability of …
Optochin Susceptibility Test Optochin Susceptibility Test uses for presumptive identification of Streptococcus pneumoniae from viridans streptococci. Principle of Optochin Susceptibility Test Ethylhydrocupreine hydrochloride (Optochin), a quinine derivative, selectively inhibits the growth of S. pneumoniae at a very low concentration (5ug/ml). Optochin may also inhibit other viridians streptococci, but only …
Introduction of Acetate Utilization Test Acetate Utilization Test ueses to determine the ability of an organism to use acetate as the sole source of carbon same as in the citrate utilization test for Enterobacteriaceae. It is the recommended assay for the differentiation of Shigella species from Escherichia coli. Composition of …
Oxidation-Fermentation (OF) Test Introduction Hugh and Leifson developed an Oxidation-Fermentation (OF) basal medium in 1953. It is a recommended medium for the detection of oxidation or fermentation of carbohydrates by bacteria. This basal medium is useful to aid in the identification of gram-negative bacteria on …
Introduction of Nitrate or Nitrite Reduction Test The nitrate or nitrite reduction test uses to determine the ability of an organism to reduce nitrate to nitrite. Anaerobic metabolism requires an electron acceptor other than atmospheric oxygen. Many gram-negative bacteria use nitrate as the final electron acceptor. The …
Introduction of DNase Test for Bacteria DNase tests positive and negative bacteria as shown below image. This test is useful for the presumptive identification of Staphylococcus aureus which produces the enzyme deoxyribonuclease (DNase) from other Staphylococci which do not produce DNase. This test is also positive in the following …