Bacteria and Fungi: Introduction, Description, Demonstration Techniques, Differences, and Keynotes

Introduction of Bacteria and Fungi

Bacteria and Fungi Introduction, Description, Demonstration Techniques, Differences, and Keynotes
Fig. Bacteria and Fungi

They are distinct types of microorganisms with different structures and characteristics. Bacteria are single-celled prokaryotes, while fungi can be single-celled (yeasts) or multicelled (molds, mushrooms) eukaryotes. They can be distinguished by their cell structure, colony morphology, and staining properties. 

Description

1. Cell Structure:

  • Bacteria lack a nucleus and other membrane-bound organelles. Their genetic material (DNA) is a single circular molecule in the cytoplasm. 
  • Fungi: Have a nucleus and other membrane-bound organelles. Fungal cells can be either unicellular (like yeast) or multicellular (like molds and mushrooms). 

2. Size:

  • Bacteria are generally smaller, ranging from 0.5 to 5 micrometers.
  • Fungi can be microscopic (e.g., yeast) or macroscopic (e.g., mushrooms). 

3. Colony Morphology:

  • Bacteria: Typically form small, creamy, or opaque colonies on agar plates.
  • Fungi: Often form larger, fuzzy, or mold-like colonies. 

4. Staining:

  • Gram Staining: A common method to differentiate bacteria based on their cell wall composition. Gram-positive bacteria retain a purple dye (crystal violet), while Gram-negative bacteria do not and appear pink (due to a counterstain). 
Gram positive bacteria (violet) and Gram negative bacteria(red)
Fig. Gram-positive bacteria (violet) and Gram-negative bacteria(red) in Gram staining
  • Simple Staining: It can be used to visualize them using a single dye. For example, methylene blue, basic fuchsin, or crystal violet can be used. 
Aspergillus in LPCB preparation
Fig. Aspergillus in LPCB preparation

5. Interactions: They can interact in various ways, including symbiotic relationships where they benefit each other, or antagonistic relationships where they compete or inhibit each other. 

6. Examples:

 Demonstration Techniques

  • Microscopy: Direct observation of bacterial and fungal cells under a microscope is a fundamental technique. 
  • Staining: Using dyes to highlight specific cellular components allows for better visualization and differentiation. 
  • Colony Morphology: Observing and characterizing the appearance of bacterial and fungal colonies on agar plates is a key identification method. 
Bacteria-Mucoid-lactose fermenting colony of Klebsiella pneumoniae on CLED agar of urine culture
Fig. Bacteria-Mucoid-lactose fermenting colony of Klebsiella pneumoniae on CLED agar of urine culture
Fungus, Aspergillus niger colony morphology on Sabouraud dextrose agar (SDA)
Fig. Fungus, Aspergillus niger colony morphology on Sabouraud dextrose agar (SDA)
  • Biochemical Tests: Various tests can be performed to identify specific bacterial and fungal species based on their metabolic activities and other characteristics. 

Differences

FeatureBacteriaFungi
Cell TypeProkaryoticEukaryotic
NucleusAbsentPresent
OrganellesAbsent (except ribosomes)Present (mitochondria, etc.)
SizeTypically smaller (0.5-5 micrometers)Typically larger (2-10 micrometers)
Cell WallPeptidoglycanChitin (usually)
ReproductionBinary fissionSpores (sexual or asexual)
NutritionAutotrophic or heterotrophicHeterotrophic (mostly decomposers)
MovementFlagella, pili, or glidingHyphal growth (most fungi)

Keynotes

  1. Cell Type: Bacteria are prokaryotic (lacking a nucleus and membrane-bound organelles), while fungi are eukaryotic (possessing a nucleus and organelles).
  2. Cell Structure: Bacteria are primarily unicellular, while fungi are mostly multicellular (though some, like yeast, are unicellular).
  3. Cell Wall: Bacterial cell walls are composed of peptidoglycan, whereas fungal cell walls are made of chitin.
  4. Nutritional Mode: Bacteria can be autotrophic (producing their food) or heterotrophic (consuming other organisms), while fungi are predominantly heterotrophic, absorbing nutrients from their surroundings.
  5. Reproduction: Bacteria typically reproduce asexually via binary fission, while fungi can reproduce both sexually and asexually (e.g., through spores).
  6. Size: Bacteria generally range from 0.5 to 5 μm, while fungi are typically 2 to 10 μm in size.
  7. Ecological Roles: Both bacteria and fungi play crucial roles in decomposition, nutrient cycling, and various symbiotic relationships.
  8. Disease: Both can cause diseases in humans, animals, and plants, although the specific pathogens and mechanisms differ.
  9. Beneficial Uses: They are utilized in various industrial processes, such as fermentation (bread, beer, cheese), antibiotic production, and bioremediation.
  10. Interactions: They can interact in complex ways, including competition, mutualism, and antagonism, influencing the overall microbial community structure and function.

Further Readings

  • https://byjus.com/biology/kingdom-fungi/
  • https://covm.uobaghdad.edu.iq/wp-content/uploads/sites/25/2023/09
  • https://www.sciencedirect.com/science/article/pii/B978008047514150010X
  • https://www.wikidoc.org/index.php/Bacteria
  • https://academic.oup.com/femsre/article/29/4/795/493265#
  • https://www.cd-genomics.com/microbioseq/how-to-distinguish-bacteria-and-fungi-from-morphology-to-sequencing.html
  • https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-023-01708-6
  • https://www.cd-genomics.com/microbioseq/how-to-distinguish-bacteria-and-fungi-from-morphology-to-sequencing.html
  • https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/rcm.721
  • https://www.bbc.co.uk/bitesize/guides/zyhrng8/revision/3
  • https://www.vedantu.com/neet/difference-between-bacteria-and-fungi
  • https://brainly.com/question/42601751
  • https://www.britannica.com/science/microbiology/Types-of-microorganisms
  • https://www.quora.com/What-are-the-differences-between-fungi-and-bacteria
  • https://study.com/academy/lesson/what-is-eubacteria-definition-characteristics-examples.
  • htmlhttps://en.wikipedia.org/wiki/Fungus

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