Introduction of Lactophenol Cotton Blue (LPCB) Stain
Table of Contents
LPCB stands for lactophenol cotton blue and Lactophenol Cotton Blue (LPCB) Stain is a combination of fixative, staining, and clearing agent. LPCB stain uses both as a mounting fluid and a stain and it is used for staining and microscopic identification of fungi. Its contents are lactic acid, phenol, cotton blue, and glycerol. Lactic acid helps in preserving the morphology of the fungal elements. Phenol acts as a disinfectant. Cotton blue stains the fungal elements as well as intestinal parasitic (cyst, ova, and oocyst) and non-parasitic structures (vegetable cells, mucus, muscle fibers, and other artifacts) while glycerol is a hygroscopic agent that prevents drying.

Principle of Lactophenol Cotton Blue (LPCB) Stain
Ingredients of lactophenol cotton blue (LPCB) stain-like lactic acid act as a clearing agent and aid in preserving the fungal structures. Similarly, phenol kills the organism and fixes it while glycerol prevents drying. Cotton blue stains the chitin in the cell wall of fungi and identification of filamentous fungi is made by their characteristic microscopic morphology e.g. shape, size, arrangement of spores, and hyphae providing color to the structure. It can be used alone or in conjunction with KOH.
Composition of Lactophenol Cotton Blue (LPCB) Stain
For 200 ml
Lactic acid: 40 ml
Phenol: 40 ml
Glycerol:80 ml
Cotton blue (Poirier blue or Aniline blue): 0.1 g
Distilled water: 40 ml
LPCB Stain Preparation
- Dissolve phenol in lactic acid, glycerol, and distilled water.
- Ultimately, add cotton blue and mix well.
- But this LPCB stain is prepared over two days.
- On the first day, dissolve the cotton blue in distilled water and leave it overnight to get rid of the insoluble dye.
- On the second day, wearing gloves add the phenol crystals to the lactic acid in a glass beaker. Put on a magnetic stirrer until the phenol is dissolved or do manually.
- Add the glycerol.
- Filter the cotton blue and distilled water (D/W) solution into the phenol/glycerol/ lactic acid solution.
- Mix and store at room temperature.
Requirements for Lactophenol Cotton Blue (LPCB) Staining
- Compound light microscope
- LPCB stain
- Clean and grease-free microscopic slides
- Coverslip
- Dropper or bamboo sticks
- Fungal growth in the medium
The procedure of LPCB Preparation
- Take a clean and grease-free glass slide.
- Put a large drop of LPCB with a Pasteur pipette or dropper.
- Transfer a small quantity of the culture to the drop.
- Tease the culture (in case of a mold) well with teasing needles
so as to get a uniform spread. - Put on a coverslip gently to avoid entrapment by air bubbles.
- Examine under low- (10 X) and high-power (40 X) objectives.
- Observe the morphological features carefully as shown below.
Observation
Fungal structures appear dark blue.
Results and interpretations of Lactophenol Cotton Blue (LPCB) Stain
Different fungi under LPCB wet mount will show different types of morphological structures including hyphae and spores
- Fungal spores, hyphae, and fruiting structures: Takes stained blue
- Background: stains pale blue.
Application of Lactophenol Cotton Blue (LPCB) Stain
- For staining and microscopic identification of fungi observing fungal spores, hyphae, and fruiting structures.
- It is also applicable in parasitology for the observation of Cysts of intestinal protozoa and ova take blue color while ova of helminths are stained deep blue.
Keynotes on LPCB Stain
- LPCB wet mount is always examined at least 30 minutes after preparation.
- A wet mount preparation should neither be too thick nor too thin.
- In this preparation, both bile-stained and non-bile-stained helminthic eggs are stained blue.
- LPCB kills the trophozoites of Entamoeba and Trichomonas, hence, can not be demonstrated by this.
- In LPCB wet mount of stool phenol and lactic clear fecal debris.
- In the LPCB wet mount of stool, glycerol provides a semi-permanent preparation. Cyst of intestinal protozoa and ova takes blue color while ova of helminths are stained deep blue. An additional advantage of this stain is that it can also detect blue-colored Cyclospora and Isospora oocyst.
Limitations of LPCB Stain
Even though LPCB stain is very useful has some demerits-
- LPCB stain is only applicable for the presumptive identification method of fungi.
- The ingredient of the LPCB solution may disrupt the original morphology of the fungi.
- The stain can only be used to identify mature fungi and their structures and not the young vegetative forms of fungi.
- A wet mount preparation should neither be too thick nor too thin.
- Application of LPCB stain in Parasitology is not preferred because it kills the trophozoites of Entamoeba and Trichomonas.
- This LPCB stain has an expiry date and thus can only use before expiry.
LPCB Stained Footages
Cryptococcus neoformans in LPCB Tease mount Microscopy

Geotrichum candidum in LPCB tease mount showing arthroconidia

Malassezia in LPCB tease mount preparatiion Microscopy

Aspergillus structures like septate hyphae, conidiophore or stipe, vesicle, Phialides, conidia, metulae, dichotomous branching (at an angle of approximately 45°) in LPCB Tease Mount Microscopy

Aspergillus fumigatus mycelium and conidia in LPCB-stained Microscopic Footage

Further Readings on LPCB Stain
- Medical Mycology. Editors: Emmons and Binford, 2nd ed 1970, Publisher Lea and Febiger, Philadelphia.
- Practical Laboratory Mycology. Editors: Koneman E.W. and G.D. Roberts, 3rd ed 1985, Publisher Williams and Wilkins, Baltimore.
- Mackie and Mc Cartney Practical Medical Microbiology. Editors: J.G. Colle, A.G. Fraser, B.P. Marmion, A. Simmons, 4th ed, Publisher Churchill Living Stone, New York, Melborne, Sans Francisco 1996.
- Clinical Microbiology Procedure Handbook, Chief in editor H.D. Isenberg, Albert Einstein College of Medicine, New York, Publisher ASM (American Society for Microbiology), Washington DC.
- A Text-Book of Medical Mycology. Editor: Jagdish Chander. Publication Mehata, India.
- Bailey & Scott’s Diagnostic Microbiology. Editors: Bettey A. Forbes, Daniel F. Sahm & Alice S. Weissfeld, 12th ed 2007, Publisher Elsevier.
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1706009/
- https://mycology.adelaide.edu.au/laboratory/lacto/
- http://himedialabs.com/TD/S016.pdf
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2024娛樂城的創新趨勢
隨著2024年的到來,娛樂城業界正經歷著一場革命性的變遷。這一年,娛樂城不僅僅是賭博和娛樂的代名詞,更成為了科技創新和用戶體驗的集大成者。
首先,2024年的娛樂城極大地融合了最新的技術。增強現實(AR)和虛擬現實(VR)技術的引入,為玩家提供了沉浸式的賭博體驗。這種全新的遊戲方式不僅帶來視覺上的震撼,還為玩家創造了一種置身於真實賭場的感覺,而實際上他們可能只是坐在家中的沙發上。
其次,人工智能(AI)在娛樂城中的應用也達到了新高度。AI技術不僅用於增強遊戲的公平性和透明度,還在個性化玩家體驗方面發揮著重要作用。從個性化遊戲推薦到智能客服,AI的應用使得娛樂城更能滿足玩家的個別需求。
此外,線上娛樂城的安全性和隱私保護也獲得了顯著加強。隨著技術的進步,更加先進的加密技術和安全措施被用來保護玩家的資料和交易,從而確保一個安全可靠的遊戲環境。
2024年的娛樂城還強調負責任的賭博。許多平台採用了各種工具和資源來幫助玩家控制他們的賭博行為,如設置賭注限制、自我排除措施等,體現了對可持續賭博的承諾。
總之,2024年的娛樂城呈現出一個高度融合了技術、安全和負責任賭博的行業新面貌,為玩家提供了前所未有的娛樂體驗。隨著這些趨勢的持續發展,我們可以預見,娛樂城將不斷地創新和進步,為玩家帶來更多精彩和安全的娛樂選擇。
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Vote justification forms provide transparency in decision-making. Members articulate their reasoning, fostering a culture of openness and ensuring that decisions are well-founded and understood by the academic community.
2. How can absentee ballots be cast?
To accommodate absentee voting, PTCs may implement secure electronic methods or designated proxy voters. This ensures that faculty members who cannot physically attend meetings can still contribute to decision-making processes.
3. How will additional members of PTCs be elected in departments with fewer than four tenured faculty members?
In smaller departments, creative solutions like rotating roles or involving faculty from related disciplines can be explored. Flexibility in election procedures ensures representation even in departments with fewer tenured faculty members.
4. Can a faculty member on OCSA or FML serve on a PTC?
Faculty members involved in other committees like the Organization of Committee on Student Affairs (OCSA) or Family and Medical Leave (FML) can serve on a PTC, but potential conflicts of interest should be carefully considered and managed.
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A quorum, the minimum number of members required for a valid meeting, is essential for decision-making. Specific rules about quorum size are usually outlined in the PTC’s governing documents.
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Understanding the processes and protocols within a Professional Tenure Committee (PTC) is crucial for faculty members. This Frequently Asked Questions (FAQ) guide aims to address common queries related to PTC procedures, voting, and membership.
1. Why should members of the PTC fill out vote justification forms explaining their votes?
Vote justification forms provide transparency in decision-making. Members articulate their reasoning, fostering a culture of openness and ensuring that decisions are well-founded and understood by the academic community.
2. How can absentee ballots be cast?
To accommodate absentee voting, PTCs may implement secure electronic methods or designated proxy voters. This ensures that faculty members who cannot physically attend meetings can still contribute to decision-making processes.
3. How will additional members of PTCs be elected in departments with fewer than four tenured faculty members?
In smaller departments, creative solutions like rotating roles or involving faculty from related disciplines can be explored. Flexibility in election procedures ensures representation even in departments with fewer tenured faculty members.
4. Can a faculty member on OCSA or FML serve on a PTC?
Faculty members involved in other committees like the Organization of Committee on Student Affairs (OCSA) or Family and Medical Leave (FML) can serve on a PTC, but potential conflicts of interest should be carefully considered and managed.
5. Can an abstention vote be cast at a PTC meeting?
Yes, PTC members have the option to abstain from voting if they feel unable to take a stance on a particular matter. This allows for ethical decision-making and prevents uninformed voting.
6. What constitutes a positive or negative vote in PTCs?
A positive vote typically indicates approval or agreement, while a negative vote signifies disapproval or disagreement. Clear definitions and guidelines within each PTC help members interpret and cast their votes accurately.
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In the current cryptocurrency sector, guaranteeing deal transparency and adherence with AML and Know Your Customer (KYC) standards is vital. Following is an overview of well-known sites that offer tools for digital asset deal monitoring, verification, and resource security.
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6. AML Node
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7. Btrace.AMLcrypto.io
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Our site also evaluates different services offering check tools for Tether deals and holdings:
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– **AML Verification for USDT:** Options are offered for tracking for fraudulent transactions.
– **“Cleanliness” Screenings for Wallets:** Verification of transaction and account legitimacy is provided to identify potential dangers.
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Selecting the suitable tool for validating and monitoring crypto transactions is essential for guaranteeing safety and compliance conformity. By consulting our recommendations, you can select the ideal tool for deal tracking and resource protection.
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1. 대행 서비스 주요 기능
메인 서비스는 고객이 한 번에 필요한 대행 서비스를 신청할 수 있도록 다양한 기능을 제공합니다.
배송대행 신청: 국내외 상품 배송을 대신 처리하며, 효율적인 시스템으로 신속한 배송을 보장합니다.
구매대행 신청: 원하는 상품을 대신 구매해주는 서비스로, 고객의 수고를 줄입니다.
엑셀 대량 등록: 대량 상품을 엑셀로 손쉽게 등록 가능하여 상업 고객의 편의성을 증대합니다.
재고 관리 신청: 창고 보관 및 재고 관리를 통해 물류 과정을 최적화합니다.
2. 고객 지원 시스템
메인 서비스는 사용자 친화적인 접근성을 제공합니다.
유저 가이드: 대행 서비스를 더욱 합리적으로 사용할 수 있도록 세부 안내서를 제공합니다.
운송장 조회: 일본 사가와 등 주요 운송사의 추적 시스템과 연동하여 운송 상황을 실시간으로 확인 가능합니다.
3. 비용 안내와 부가 서비스
비용 계산기: 예상되는 비용을 간편하게 계산해 예산 관리를 돕습니다.
부가 서비스: 교환 및 반품, 폐기 및 검역 지원 등 추가적인 편의 서비스를 제공합니다.
출항 스케줄 확인: 해외 배송의 경우 출항 일정을 사전에 확인 가능하여 배송 계획을 세울 수 있습니다.
4. 공지사항
기본 검수 공지
무료 검수 서비스로 고객의 부담을 줄이며, 보다 철저한 검수가 필요한 경우 유료 정밀 검수 서비스를 권장합니다.
수출허가서 발급 안내
항공과 해운 수출 건에 대한 허가서를 효율적으로 발급받는 방법을 상세히 안내하며, 고객의 요청에 따라 이메일로 전달됩니다.
노데이터 처리 안내
운송장 번호 없는 주문에 대한 새로운 처리 방안을 도입하여, 노데이터 발생 시 관리비가 부과되지만 서비스 품질을 개선합니다.
5. 고객과의 소통
카카오톡 상담: 실시간 상담을 통해 고객의 궁금증을 해결합니다.
공지사항 알림: 서비스 이용 중 필수 정보를 지속적으로 업데이트합니다.
메인 서비스는 고객 만족을 최우선으로 하며, 지속적인 개선과 세심한 관리를 통해 최상의 경험을 제공합니다.
일본 소비세 환급, 네오리아와 함께라면 간편하고 안전하게
일본 소비세 환급은 복잡하고 까다로운 절차로 많은 구매대행 셀러들이 어려움을 겪는 분야입니다. 네오리아는 다년간의 경험과 전문성을 바탕으로 신뢰할 수 있는 서비스를 제공하며, 일본 소비세 환급 과정을 쉽고 효율적으로 처리합니다.
1. 일본 소비세 환급의 필요성과 네오리아의 역할
네오리아는 일본 현지 법인을 설립하지 않아도 합법적인 방식으로 소비세 환급을 받을 수 있는 솔루션을 제공합니다. 이를 통해:
한국 개인사업자와 법인 사업자 모두 간편하게 환급 절차를 진행할 수 있습니다.
일본의 복잡한 서류 심사를 최소화하고, 현지 로컬 세리사와 협력하여 최적의 결과를 보장합니다.
2. 소비세 환급의 주요 특징
일본 연고가 없어도 가능: 일본에 사업자가 없더라도 네오리아는 신뢰할 수 있는 서비스를 통해 소비세 환급을 지원합니다.
서류 작성 걱정 해결: 잘못된 서류 제출로 환급이 거절될까 걱정될 필요 없습니다. 네오리아의 전문 대응팀이 모든 과정을 정밀하게 관리합니다.
현지 법인 운영자를 위한 추가 지원: 일본 내 개인사업자나 법인 운영자에게는 세무 감사와 이슈 대응까지 포함된 고급 서비스를 제공합니다.
3. 네오리아 서비스의 장점
전문성과 신뢰성: 정부로부터 인정받은 투명성과 세무 분야의 우수한 성과를 자랑합니다.
맞춤형 서포트: 다양한 사례를 통해 쌓은 경험으로 고객이 예상치 못한 어려움까지 미리 해결합니다.
로컬 업체에서 불가능한 고급 서비스: 한국인 고객을 위해 정확하고 간편한 세무회계 및 소비세 환급 서비스를 제공합니다.
4. 네오리아가 제공하는 혜택
시간 절약: 복잡한 절차와 서류 준비 과정을 전문가가 대신 처리합니다.
안심 환급: 철저한 관리와 세심한 대응으로 안전하게 환급을 받을 수 있습니다.
추가 서비스: 세무감사와 이슈 발생 시 즉각적인 지원으로 사업의 연속성을 보장합니다.
네오리아는 소비세 환급이 복잡하고 어렵다고 느껴지는 고객들에게 최적의 길잡이가 되어드립니다. 신뢰를 바탕으로 한 전문적인 서비스로, 더 이상 소비세 환급 문제로 고민하지 마세요!
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