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Introduction of LF and NLF Gram-Negative Bacteria on MacConkey Medium MacConkey agar/ medium is recommended for use as a selective, differential, and indicator growth medium for the isolation of gram-negative bacilli including coliform organisms and enteric pathogens, on the basis of lactose fermentation. Peptones (meat …
Introduction “Negative Pressure HEPA Filtration Unit” is useful for hospital (isolation ward that keeps patients with infectious illnesses or immunocompromised individuals who are susceptible to infections from others, away from other patients, visitors, and healthcare workers) and molecular laboratory (Sample Aliquoting and RNA Extraction Room …
Introduction of Staphylococcus and Micrococcus Staphylococcus (pleural-staphylococci) is spherical, non-motile, gram-positive in singles, pairs, and clusters. On nutrient agar, growth is opaque and golden yellow or white color. Catalase and coagulase test positive (Staphylococcus aureus), oxidase negative, aerobic or facultative anaerobe. Parasite of humans and …
Introduction of Streptococcus pneumoniae and Viridans streptococci Streptococcus pneumoniae is also called pneumococcus and pneumococcus is the commensals of the upper respiratory tract. It is an important etiological agent of pneumonia and otitis media in children that differs from streptococci in its morphology (diplococci), bile …
Introduction of Amoebic dysentery and Bacillary dysentery Amoebic dysentery or amoebiasis is an intestinal infection caused by a unicellular (protozoan) parasite, Entamoeba histolytica whereas bacillary dysentery or shigellosis is a bacterial disease caused by a group of non-motile bacteria called Shigella species. Differences between Amoebic …
History and Introduction of Mueller-Hinton Agar (MHA) The name Mueller- Hinton agar(MHA) is from the surname of co-developers microbiologist John Howard Mueller and veterinary scientist Jane Hilton at Harvard University as a culture for gonococcus and meningococcus, who published the method in 1941. It is a non-selective and non-differential …
Introduction of Chocolate agar Chocolate Agar short form is (CHOC). It is a non-selective, enriched growth medium that is the lysed blood agar. The agar is named for its color when the red blood cells (RBCs) lysis gives the medium a chocolate-brown color without having chocolate …
Introduction of Blood Agar Blood agar is an enriched medium for the cultivation of bacteria. Fastidious organisms, such as streptococci, do not grow well on ordinary growth media. It is a type of growth medium i.e. trypticase soy agar enriched with 5% sheep blood or blood agar base with 5-10 % sterile …