This site is fully related to medical laboratory information for public and health care professionals.
Introduction of Nutrient Agar Nutrient agar is a simple medium which uses to grow bacteria. It is devoid of indicators, selective agents, differential ingredients, and enriching substances therefore uses for better expression of pigmentation, biochemical test, and even serotyping. The short form of nutrient agar …
Introduction of String Test String test is a special test of bacteria to identify Vibrio cholerae (String test positive) from Vibrio species and other bacteria like Aeromonas species and Plesiomonas shigelloides. This assay can perform on a glass slide or plastic petri dish, suspending overnight …
Triple Sugar Iron Agar Test The triple Sugar Iron (TSI) Agar test is a combo test and is useful for the identification of mainly gram-negative bacteria, on the basis of the following tests- Composition of Triple Sugar Iron Agar Triple Sugar Iron Agar (TSI) (Oxoid, England) …
Introduction of Lactophenol Cotton Blue (LPCB) Stain LPCB stands for lactophenol cotton blue and Lactophenol Cotton Blue (LPCB) Stain is a combination of fixative, staining, and clearing agent. LPCB stain uses both as a mounting fluid and a stain and it is used for staining …
Introduction Satellitism test or satellite test is applied to identify Haemophilus influenzae. It is a fastidious bacterium and thus it needs extra ingredients like hemin (factor X) and NAD (factor V) to grow. It uses the X factor to produce essential respiratory enzymes such as cytochromes, catalases, …
Introduction Methylene Blue Stain, also known as methylene blue aqueous is an ordinary stain. It is used for capsule stain as well as simple staining for a rapid and easy way to determine cell shape, size, and arrangements of bacteria. It is also made easier to …
Ziehl -Neelsen Stain Ziehl–Neelsen stain is a type of acid-fast stain and it is named after the surnames of two German doctors who modified the stain. One is the bacteriologist Franz Ziehl (1859–1926) and other is the pathologist Friedrich Neelsen (1854–1898). It was initially introduced …
Introduction SIM stands for sulfide, Indole, and Motility and SIM medium is applied for the differentiation of gram-negative enteric bacilli. SIM test assists to isolate the bacteria on the basis of sulfide production, indole formation, and motility and thus SIM test is a combination of three …