fungal stain

Nail Clippings KOH Mount: Introduction and Principle, Procedure, Result-Interpretation, Clinical Significance, and Keynotes

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Hyaline septate fungal hyphae in KOH mount of nail clipping at a magnification of 1000x with 8x optical zoom

Introduction and Principle A nail clipping KOH (Potassium Hydroxide) mount is the fastest and most cost-effective diagnostic test used to detect fungal elements in nails. It serves as the primary screening tool for confirming onychomycosis (fungal nail infection) before starting long-term oral antifungal therapy The …

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Grocott’s Methenamine Silver (GMS) Staining of Mycobacteria:Introduction, Principle, Clinical Significance, Advantage, Disadvantage, and Keynotes

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Introduction Grocott’s Methenamine Silver (GMS) stain is a histochemical staining method primarily used to detect fungi in tissue sections. However, it can also stain certain acid-fast organisms like Mycobacteria, especially in tissue biopsies where standard Ziehl-Neelsen or fluorescent stains may miss them. The technique enhances …

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