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Introduction of Enterobacteriaceae The family Enterobacteriaceae was first proposed by Rahn in 1936 and it is a very large group of gram-negative bacteria that possess a number of common morphological and biochemical properties and similar DNA base compositions. The widely used systems for the classification …
Introduction of LF and NLF Gram-Negative Bacteria on MacConkey Medium MacConkey agar/ medium is recommended for use as a selective, differential, and indicator growth medium for the isolation of gram-negative bacilli including coliform organisms and enteric pathogens, on the basis of lactose fermentation. Peptones (meat …
Introduction of Haemophilus The genus Haemophilus is a pleomorphic Gram-negative coccobacilli fastidious bacteria and thus they do not grow on an ordinary medium like nutrient agar and they need chocolate agar to grow (H. influenzae) due to having X, V, and both X and V growth factors in it. …
Introduction of Amoebic dysentery and Bacillary dysentery Amoebic dysentery or amoebiasis is an intestinal infection caused by a unicellular (protozoan) parasite, Entamoeba histolytica whereas bacillary dysentery or shigellosis is a bacterial disease caused by a group of non-motile bacteria called Shigella species. Differences between Amoebic …
History and Introduction of Mueller-Hinton Agar (MHA) The name Mueller- Hinton agar(MHA) is from the surname of co-developers microbiologist John Howard Mueller and veterinary scientist Jane Hilton at Harvard University as a culture for gonococcus and meningococcus, who published the method in 1941. It is a non-selective and non-differential …
Introduction of Chocolate agar Chocolate Agar short form is (CHOC). It is a non-selective, enriched growth medium that is the lysed blood agar. The agar is named for its color when the red blood cells (RBCs) lysis gives the medium a chocolate-brown color without having chocolate …
Introduction of Blood Agar Blood agar is an enriched medium for the cultivation of bacteria. Fastidious organisms, such as streptococci, do not grow well on ordinary growth media. It is a type of growth medium i.e. trypticase soy agar enriched with 5% sheep blood or blood agar base with 5-10 % sterile …
Introduction of Nutrient Agar Nutrient agar is a simple medium which uses to grow bacteria. It is devoid of indicators, selective agents, differential ingredients, and enriching substances therefore uses for better expression of pigmentation, biochemical test, and even serotyping. The short form of nutrient agar …