Procedure Archives - Page 4 of 6

Bile Solubility Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, and Keynotes

January 13, 2023January 13, 2023 by Medical Lab Notes

Bile Solubility Test- Positive and Negative

Introduction of Bile Solubility Test Bile solubility test uses for presumptive identification of Streptococcus pneumoniae. Principle of Bile Solubility Test Bile salts, specifically sodium deoxycholate and sodium taurocholate, have the capacity to selectively lyse Streptococcus pneumoniae when added to actively growing bacteria in agar or broth media. S. pneumoniae produces autolytic …

Oxidation-Fermentation (OF) Test: Introduction, Composition of the medium, Test Requirements, Principle, Procedure, Result -Interpretation, and Limitations

January 13, 2023January 6, 2023 by Medical Lab Notes

Fermentation Test Positive and Negative Results of Bacteria

Oxidation-Fermentation (OF) Test Introduction Hugh and Leifson developed an Oxidation-Fermentation (OF) basal medium in 1953. It is a recommended medium for the detection of oxidation or fermentation of carbohydrates by bacteria. This basal medium is useful to aid in the identification of gram-negative bacteria on …

Hematoxylin and Eosin (H&E) Stain: Introduction, Principle, Test Requirements, Procedure, Result- Interpretation, and Keynotes

November 26, 2022October 19, 2022 by Medical Lab Notes

Introduction of Hematoxylin and Eosin Stain Hematoxylin and eosin stain is abbreviated as H&E stain. It is one of the principal tissue stains used in histopathology to find out cancer from a suspected biopsy. The stain was first introduced by A. Wissowzky in 1876. The cell nuclei …

SARS‑CoV‑2 RNA Extraction using Biocomma Kit: Introduction, Reagent Preparation, Extraction Requirements, Procedure, and Keynotes

November 26, 2022September 7, 2022 by Medical Lab Notes

Introduction of SARS‑CoV‑2 RNA Extraction using Biocomma Kit Biocomma nucleic acid (DNA/RNA) extraction kit is a Chinese product that is useful for RNA extraction systems for COVID-19 detection. It is applied for manual extraction and it is completed in four steps- A kit (for 50 …

Dengue Rapid Test for IgG and IgM Detection: Introduction, Principle, Test Requirements, Procedure, Result Interpretation, and Limitations

November 29, 2022September 6, 2022 by Medical Lab Notes

Introduction of Dengue IgG and IgM Rapid Test Dengue rapid test (RDT) uses for the detection of immunoglobulin G(IgG) and immunoglobulin M (IgM) where IgM is responsible for the past while IgG is for present infection as shown above picture. Dengue is a mosquito-borne viral …

Indole test: Introduction, Principle, Test Requirements, Procedure, Result -Interpretation, and Keynotes

December 16, 2022August 18, 2022 by Medical Lab Notes

Indole Production test This indole test is useful to identify the bacteria on the basis of indole production. The list of indole-positive organisms is- Principle of Indole test The ability of an organism to split Indole from the amino acid tryptophan is due to the presence of …

ASO (Anti-streptococcal O)Test: Introduction, Principle, Procedure, Normal Range, Result- Interpretation, and Keynotes

November 29, 2022August 9, 2022 by Medical Lab Notes

Introduction of ASO Test ASO stands for Antistreptococcal O and it is an antibody targeted against streptolysin O, a toxic enzyme produced by group A Streptococcus bacterium. Antistreptococcal O and anti -DNase are the most common of several antibodies that are produced by the body’s immune system in response to a strep infection …

Enzyme-Linked Immunosorbent Assay (ELISA): Introduction, Principle, Types, Procedure, Result-Interpretation, Advantage, Disadvantage, and Interfering Factors of ELISA Test Results

November 29, 2022August 9, 2022 by Medical Lab Notes

Introduction of ELISA ELISA stands for Enzyme-Linked Immunosorbent Assay. It comes under antigen and antibody reaction tests and is useful for the identification of antigens or antibodies of the following specimens serum, urine, CSF, sputum, semen, supernatant of culture, stool, etc. It is also applicable …