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Principle of Decarboxylase-Dihydrolase Test Lysine, ornithine, and arginine are the most common amino acids which use to detect an organism’s ability to decarboxylate or hydrolyze an amino acid in a decarboxylase medium, forming an amine that produces an alkaline pH. The basal medium for the …
Introduction of Methyl-α-D-glucopyranoside (MGP)Test Methyl-α-D-glucopyranoside (MGP) test is effective for species identification of Enterococcus. Enterococcus gallinarum and Enterococcus casseliflavus are MGP test positive while Enterococcus faecalis and Enterococcus faecium are negative. Vancomycin-resistant Enterococcus faecium (VREF) is a serious nosocomial problem that is difficult to differentiate from Enterococcus gallinarum and Enterococcus casseliflavus, while not important for purposes of control of …
Introduction of Indoxyl Acetate Disk Test Indoxyl is a tryptophan product of putrefactive decomposition in the intestines of humans by a bacterial esterase. This Indoxyl Acetate Disk test uses to distinguish Campylobacter species. Positive tests are Campylobacter jejuni and Campylobacter coli whereas Campylobacter lari and Campylobacter fetus are negative. Principle of Indoxyl Acetate Disk Assay The …
Introduction of Hippurate Hydrolysis Test The Hippurate Hydrolysis Test is applied to determine the ability of the organism to produce hippuricase which hydrolyzes the substrate hippurate. It is useful in the identification of Streptococcus agalactiae, Campylobacter jejuni, Listeria monocytogenes, and Gardnerella vaginalis. Principle of the Test The end products …
Introduction of LAP (Leucine Aminopeptidase) Test LAP stands for leucine aminopeptidase which is an enzyme useful for definitive identification of catalase-negative, gram-positive cocci of Aerococcus and Leuconostoc (LAP test negative) from other organisms in the group like Streptococcus, Enterococcus, Lactococcus, and Pediococcus (almost always positive). Principle of LAP test Hydrolysis of the leucine-pnaphthylamide substrate by …
Introduction of Microbiology Reporting Methods Microbiology Reporting Methods are useful to provide similar report formats from different Microbiology Laboratory platforms that should sound Microbiologically clear and concise. It should also be compatible with International Guidelines of Clinical Microbiology Practice like the Clinical & Laboratory Standards …
Introduction of XLD Agar XLD agar is a selective, differential, and indicator medium for the isolation of enteric pathogens. XLD stands for xylose lysine deoxycholate. It also supports the growth of more fastidious enteric organisms. XLD agar was developed by Taylor. It was specially designed to allow the …
Introduction of Mannitol salt agar (MSA) Mannitol Salt Agar (MSA) is a selective, differential, and indicator medium which is used to isolate and identify S. aureus from clinical and non-clinical specimens. Other Staphylococcus species and Micrococcus also grow on this medium. Selective is due to very high salt (7.5%) compared with other media. Gram-Positive Staphylococcus: …