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Introduction SIM stands for sulfide, Indole, and Motility and SIM medium is applied for the differentiation of gram-negative enteric bacilli. SIM test assists to isolate the bacteria on the basis of sulfide production, indole formation, and motility and thus SIM test is a combination of three …
Introduction The urease test is also known as the urea hydrolyzation test or urea test and it is important for certain bacteria and fungi that possess this enzyme which hydrolyzes urea releasing ammonia into the medium. This produces a change in the pH of the …
Introduction of Gram Stain Gram stain is the most commonly used routine stain in microbiology for the identification of microbes and also to know the evidence of etiological agents in clinical specimens. Gram staining is a differential stain and thus it applies to differentiate Gram-positive …
Introduction of Coagulase Test The coagulase test is an enzymatic assay that is applied to differentiate Staphylococcus aureus (positive) from coagulase-negative staphylococci (CoNS). Coagulase is an enzyme formed by S. aureus which changes soluble fibrinogen of plasma to insoluble fibrin. S. aureus produces two forms of coagulase. One is bound coagulase …
Introduction A citrate utilization test is the most commonly used routine biochemical test for Gram-negative rods in bacteriology for the identification of bacteria on the basis of citrate utilization. Klebsiella pneumoniae is the citrate utilization-positive organism while Escherichia coli is negative. Principle of Citrate Utilization Test …
Introduction of Common Formulae of Diagnostic Laboratory The ‘Common Formulae of Diagnostic Laboratory’ title is really useful who are involved in the Department of Pathology and Laboratory Medicine tests reporting as well as students who are related to this branch of health science. The author …
Introduction of Catalase Test The catalase test is the most common in bacteriology and is commonly used to differentiate those bacteria that produce this enzyme, such as staphylococci, from non-catalase producing bacteria such as streptococci. It is two types depending on the testing methods and they …
Introduction of Oxidase Test (Kovac’s Method) Gordon and McLeod introduced the application of a dimethyl-phenylenediamine dihydrochloride solution to test for the presence of oxidase systems in 1928. In particular, they used the test to differentiate N. gonorrhoeae (oxidase test positive) from Staphylococcus species and Streptococcus species (oxidase negative). The sensitivity of the …