Fungal Elements in KOH Mount of Pleural Fluid Microscopy-Introduction, Possible Fungi Observed in Pleural Fluid KOH Mount, Applications, and Keynotes

Fungal Elements in KOH Mount of Pleural Fluid Microscopy-Introduction, Possible Fungi Observed in Pleural Fluid KOH Mount, Applications, and Keynotes

Introduction KOH Mount of Pleural Fluid is a rapid, direct microscopic test used to detect fungal elements in suspected pleural infections. A 10–20% Potassium Hydroxide (KOH) solution clears cellular debris, fibrin, and protein content in pleural fluid while preserving chitin-rich fungal structures such as hyphae, …

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Broad Range PCR Assay for Fungi-Directly on Clinical Samples: Introduction, Principle, Clinical Significance, and Keynotes

Broad Range PCR Assay for Fungi-Directly on Clinical Samples-Introduction, Principle, Clinical Significance, and Keynotes

Introduction Broad-range PCR assays for fungi are molecular diagnostic methods designed to detect a wide spectrum of fungal pathogens directly from clinical samples such as blood, BAL, sputum, CSF, tissue biopsies, and sterile body fluids.These assays target highly conserved fungal genomic regions, primarily the Internal …

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