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Introduction of Enterobacteriaceae The family Enterobacteriaceae was first proposed by Rahn in 1936 and it is a very large group of gram-negative bacteria that possess a number of common morphological and biochemical properties and similar DNA base compositions. The widely used systems for the classification …
Introduction of LF and NLF Gram-Negative Bacteria on MacConkey Medium MacConkey agar/ medium is recommended for use as a selective, differential, and indicator growth medium for the isolation of gram-negative bacilli including coliform organisms and enteric pathogens, on the basis of lactose fermentation. Peptones (meat …
Introduction of India Ink Preparation Cryptococcal meningitis occurs in immunodeficient patients and when meningitis is clinically suspected, for example, patients with HIV/AIDS, or when yeast cells with lymphocytes are detected when performing Cerebrospinal fluid (CSF) cell count or examining Gram smear, examine India ink preparation …
Introduction of Blood Agar Blood agar is an enriched medium for the cultivation of bacteria. Fastidious organisms, such as streptococci, do not grow well on ordinary growth media. It is a type of growth medium i.e. trypticase soy agar enriched with 5% sheep blood or blood agar base with 5-10 % sterile …
Introduction of Nutrient Agar Nutrient agar is a simple medium which uses to grow bacteria. It is devoid of indicators, selective agents, differential ingredients, and enriching substances therefore uses for better expression of pigmentation, biochemical test, and even serotyping. The short form of nutrient agar …
Introduction of String Test String test is a special test of bacteria to identify Vibrio cholerae (String test positive) from Vibrio species and other bacteria like Aeromonas species and Plesiomonas shigelloides. This assay can perform on a glass slide or plastic petri dish, suspending overnight …
Triple Sugar Iron Agar Test The triple Sugar Iron (TSI) Agar test is a combo test and is useful for the identification of mainly gram-negative bacteria, on the basis of the following tests- Composition of Triple Sugar Iron Agar Triple Sugar Iron Agar (TSI) (Oxoid, England) …
Introduction Methylene Blue Stain, also known as methylene blue aqueous is an ordinary stain. It is used for capsule stain as well as simple staining for a rapid and easy way to determine cell shape, size, and arrangements of bacteria. It is also made easier to …