Vibrio cholerae Serotyping: Introduction, Principle, Test Requirements, Test Procedure, Result- Interpretation and Keynotes

Vibrio cholerae Serotyping Procedure

Introduction of Vibrio cholerae Serotyping Vibrio Serotyping helps to differentiate serotypes of Vibrio. Serotypes refer to separate groups within a species of microorganisms that all share a similar property. More specifically, each serotype has the same number of antigens on its surfaces. For example, V.cholerae O1 serovar Inaba, V. cholerae O1 serovar …

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Haemophilus Serotyping: Introduction, Principle, Test Requirements, Test Procedure, Result-Interpretation, Limitations, and Keynotes

Use of bacitracin 10 U on chocolate agar for screening Haemophilus of sputum culture

Introduction of Haemophilus Serotyping Haemophilus Serotyping helps to differentiate serotypes of Haemophilus influenzae. Serotypes refer to separate groups within a species of microorganisms that all share a similar property. More specifically, each serotype has the same number of antigens on its surfaces. For example, encapsulated strains of Haemophilus influenzae may …

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Shigella Serotyping: Introduction, Principle, Test Requirements, Test Procedure, Result-Interpretation, and Keynotes

Shigella polyvalent A, B and C2 for Shigella dysenteriae, Shigella flexneri, and Shigella boydii

Introduction of Shigella Serotyping Shigella Serotyping helps to differentiate serotypes of Shigella. Serotypes refer to separate groups within a species of microorganisms that all share a similar property. More specifically, each serotype has the same number of antigens on its surfaces. For example, Shigella flexneri serotype 1, 2, 2a, S. sonnei, and S. …

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Widal Test: Introduction, Principle, Test Requirement, Procedure, Result- Interpretation, and Limitations

Widal Test Positive Demonstration

Introduction of Widal Test The Widal test is an agglutination test employed in the serological diagnosis of enteric fever. It is also applicable in febrile agglutinin tests. The test is named after Georges Fernand Isidore Widal, a French physician and bacteriologist. Salmonella antibody starts appearing in the …

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ABO and Rh Typing: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Applications, and Keynotes

ABO and Rh Typing-Slide Method

Introduction of ABO and Rh Typing ABO blood grouping and Rh typing are the most common blood group systems. There are nearly 300 blood group systems so far discovered. Other than ABO and  Rh are MNS, Lutheran, Kell, Duffy, etc. Karl Landsteiner discovered ABO ( …

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Antinuclear Antibody (ANA)Test: Introduction, Principle, Procedure, Result Interpretation, Clinical Significance, and Keynotes

Antinuclear Antibody (ANA)Test- Introduction, Principle, Procedure, Result Interpretation, Clinical Significance and Keynotes

Introduction of Antinuclear Antibody (ANA)Test The antinuclear antibody (ANA)test is a type of blood test in which serum tested for autoantibodies ( SLE) directed against native deoxyribonucleic acid and other nuclear constituents are produced. It involves a variety of tissues and is associated with a wide range …

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GeneXpert Test For Tuberculosis: Introduction, Principle, Test Requirements, Test Procedure, Result -Interpretation, Advantages, Disadvantage, and Keynotes

GeneXpert Test For Tuberculosis-Mycobacterium tuberculosis (MTB) Detected High

Introduction of GeneXpert Test GeneXpert test is a molecular test for tuberculosis diagnosis by detecting the presence of  Mycobacterium tuberculosis (bacteria), as well as testing for resistance to the drug rifampicin. Principle of GeneXpert Test GeneXpert test is a highly sensitive and specific test for tuberculosis(TB) diagnosis that detects DNA sequences …

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Decarboxylase Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes

Decarboxylase Test-Positive and Negative Results Demonstration

Principle of Decarboxylase-Dihydrolase Test Lysine, ornithine, and arginine are the most common amino acids which use to detect an organism’s ability to decarboxylate or hydrolyze an amino acid in a decarboxylase medium, forming an amine that produces an alkaline pH. The basal medium for the …

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