Principle Archives - Page 7 of 10

Methyl-α-D-glucopyranoside (MGP) Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes

February 17, 2023February 2, 2023 by Medical Lab Notes

Methyl-α-D-glucopyranoside (MGP) Test- Positive and Negative Results Demonstration

Introduction of Methyl-α-D-glucopyranoside (MGP)Test Methyl-α-D-glucopyranoside (MGP) test is effective for species identification of Enterococcus. Enterococcus gallinarum and Enterococcus casseliﬂavus are MGP test positive while Enterococcus faecalis and Enterococcus faecium are negative. Vancomycin-resistant Enterococcus faecium (VREF) is a serious nosocomial problem that is difficult to differentiate from Enterococcus gallinarum and Enterococcus casseliﬂavus, while not important for purposes of control of …

Indoxyl Acetate Disk Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes

February 17, 2023February 2, 2023 by Medical Lab Notes

Indoxyl Acetate Disk Test-Positive and Negative Results

Introduction of Indoxyl Acetate Disk Test Indoxyl is a tryptophan product of putrefactive decomposition in the intestines of humans by a bacterial esterase. This Indoxyl Acetate Disk test uses to distinguish Campylobacter species. Positive tests are Campylobacter jejuni and Campylobacter coli whereas Campylobacter lari and Campylobacter fetus are negative. Principle of Indoxyl Acetate Disk Assay The …

XLD Agar: Introduction, Composition, Principle, Preparation, Test Requirements, Procedure, Colony Characteristics, Modifications, Uses, and Limitations

February 4, 2023January 25, 2023 by Medical Lab Notes

Salmonella Typhi (black colony) and Shigella growth on xylose lysine deoxycholate (XLD) agar

Introduction of XLD Agar XLD agar is a selective, differential, and indicator medium for the isolation of enteric pathogens. XLD stands for xylose lysine deoxycholate. It also supports the growth of more fastidious enteric organisms. XLD agar was developed by Taylor. It was specially designed to allow the …

Mannitol Salt Agar (MSA): Introduction, Composition, Principle, Test Requirement, Procedure, Result -Interpretation, Limitation, and Keynotes

February 17, 2023January 25, 2023 by Medical Lab Notes

Staphylococcus aureus , CoNS and inhibited growth of Escherichia coli on MSA

Introduction of Mannitol salt agar (MSA) Mannitol Salt Agar (MSA) is a selective, differential, and indicator medium which is used to isolate and identify S. aureus from clinical and non-clinical specimens. Other Staphylococcus species and Micrococcus also grow on this medium. Selective is due to very high salt (7.5%) compared with other media. Gram-Positive Staphylococcus: …

TCBS Agar : Introduction, Composition,Principle, Test Requirement,Procedure, Result -Interpretation, Limitation, and Keynotes

January 26, 2023January 24, 2023 by Medical Lab Notes

Vibrio cholerae colony morphology on TCBS agar

Introduction of TCBS Agar Vibrio cholerae is the causative agent of cholera and Thiosulfate Citrate bile salts sucrose (TCBS) agar is a selective as well as differential culture medium used for selective isolation of this bacterium including its species from a variety of clinical and nonclinical specimens. TCBS agar …

Dermatophyte Test Medium (DTM): Introduction, Composition, Principle, Test Requirements, Procedure, Result-Interpretation, Colony Characteristics, Modifications,Uses, and Keynotes

February 17, 2023January 21, 2023 by Medical Lab Notes

Dermatophytes growth on Dermatophyte Test Medium (DTM)

Introduction of Dermatophyte Test Medium (DTM) Dermatophyte Test Medium (DTM) is a selective and differential medium recommended for the cultivation and isolation of pathogenic dermatophytes. The Dermatophytes are a distinct group of fungi that infect the hair, skin, and nails in humans producing a variety of …

Potato Dextrose Agar (PDA): Introduction, Composition, Principle, Test Requirements, Procedure, Result-Interpretation, Colony Characteristics, Modifications, Uses, and Keynotes

February 17, 2023January 21, 2023 by Medical Lab Notes

Introduction of Potato Dextrose Agar (PDA) Potato Dextrose Agar ( PDA) is a fungal medium and its ingredients are clear from its name it contains potato ( vegetable), dextrose ( sugar), and agar ( solidifying agent). PDA is recommended for the isolation and enumeration of fungi …

API Test for Bacteria: Introduction, Principle, Test Requirements, Test Procedure, Result- Interpretation, Advantages, and Keynotes

January 13, 2023January 13, 2023 by Medical Lab Notes

Introduction of API Test for Bacteria API test for bacteria as shown above picture is a 20-jumbo tests kit for a biochemical panel for the identification and differentiation of members of the family Enterobacteriaceae. API stands for Analytical Profile Index. It is an API 20E Test kit …