Principle Archives - Page 8 of 10

Bile Solubility Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, and Keynotes

January 13, 2023January 13, 2023 by Medical Lab Notes

Bile Solubility Test- Positive and Negative

Introduction of Bile Solubility Test Bile solubility test uses for presumptive identification of Streptococcus pneumoniae. Principle of Bile Solubility Test Bile salts, specifically sodium deoxycholate and sodium taurocholate, have the capacity to selectively lyse Streptococcus pneumoniae when added to actively growing bacteria in agar or broth media. S. pneumoniae produces autolytic …

Oxidation-Fermentation (OF) Test: Introduction, Composition of the medium, Test Requirements, Principle, Procedure, Result -Interpretation, and Limitations

January 13, 2023January 6, 2023 by Medical Lab Notes

Fermentation Test Positive and Negative Results of Bacteria

Oxidation-Fermentation (OF) Test Introduction Hugh and Leifson developed an Oxidation-Fermentation (OF) basal medium in 1953. It is a recommended medium for the detection of oxidation or fermentation of carbohydrates by bacteria. This basal medium is useful to aid in the identification of gram-negative bacteria on …

Hematoxylin and Eosin (H&E) Stain: Introduction, Principle, Test Requirements, Procedure, Result- Interpretation, and Keynotes

November 26, 2022October 19, 2022 by Medical Lab Notes

Introduction of Hematoxylin and Eosin Stain Hematoxylin and eosin stain is abbreviated as H&E stain. It is one of the principal tissue stains used in histopathology to find out cancer from a suspected biopsy. The stain was first introduced by A. Wissowzky in 1876. The cell nuclei …

Pap Test: Introduction, Principle, Staining Procedure, Result- Interpretation, and Keynotes

November 26, 2022October 18, 2022 by Medical Lab Notes

Introduction of Pap Test Pap test is also known as Pap smear and Pap stain. Pap stain was named after the three letters of the surname of developer George Papanicolaou in 1942 and was routinely used for cytological stain. It is the most common staining …

Bacterial Endotoxin Detection by Chromogenic Method: Introduction, Principle, Product Characteristics, and Keynotes

November 26, 2022October 17, 2022 by Medical Lab Notes

Introduction of Bacterial Endotoxin Detection by Chromogenic Method Bacterial Endotoxin Detection by Chromogenic Method uses for screening and diagnosis of Gram-negative bacterial infection with a low limit of detection is 1 pg/ml (0.005EU/ml). It also applies to the quantitative detection of endotoxin in pharmaceutical and …

Dengue Rapid Test for IgG and IgM Detection: Introduction, Principle, Test Requirements, Procedure, Result Interpretation, and Limitations

November 29, 2022September 6, 2022 by Medical Lab Notes

Introduction of Dengue IgG and IgM Rapid Test Dengue rapid test (RDT) uses for the detection of immunoglobulin G(IgG) and immunoglobulin M (IgM) where IgM is responsible for the past while IgG is for present infection as shown above picture. Dengue is a mosquito-borne viral …

Indole test: Introduction, Principle, Test Requirements, Procedure, Result -Interpretation, and Keynotes

December 16, 2022August 18, 2022 by Medical Lab Notes

Indole Production test This indole test is useful to identify the bacteria on the basis of indole production. The list of indole-positive organisms is- Principle of Indole test The ability of an organism to split Indole from the amino acid tryptophan is due to the presence of …

Incubator: Introduction, Principle, Handling Procedure, Types, Uses, and Keynotes

November 29, 2022August 11, 2022 by Medical Lab Notes

Introduction of Incubator The incubator is an electrical device in which the incubation process is performed which conditions an enclosure to a predetermined temperature since it provides and maintains all artificial optimal conditions for the growth of microbial culture as well as cell and tissue …