Culture Media

Mueller-Hinton agar (MHA): Introduction, Composition, Principle, Preparation Requirements, Testing Procedure, Colony Characteristics, Uses, Keynotes, and MHA Footages

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Mueller-Hinton agar (MHA): Introduction, Composition, Principle, Preparation Requirements, Testing Procedure, Colony Characteristics, Uses, Keynotes, and MHA Footages

History and Introduction of Mueller-Hinton Agar (MHA) The name Mueller- Hinton agar(MHA) is from the surname of co-developers microbiologist John Howard Mueller and veterinary scientist Jane Hilton at Harvard University as a culture for gonococcus and meningococcus, who published the method in 1941. It is a non-selective and non-differential …

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Chocolate Agar: Introduction, Composition, Principle, Preparation Requirements, Testing Procedure, Colony Characteristics, Uses, Keynotes, and Chocolate Agar Footages

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Chocolate Agar: Introduction,Composition, Principle, Preparation Requirements,Testing Procedure, Colony Characteristics, Uses, Keynotes and Chocolate Agar Footages

Introduction of Chocolate agar  Chocolate Agar short form is (CHOC). It is a non-selective, enriched growth medium that is the lysed blood agar. The agar is named for its color when the red blood cells (RBCs) lysis gives the medium a chocolate-brown color without having chocolate …

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Blood Agar: Introduction, Composition, Principle, Preparation Requirements, Test Procedure, Result -Interpretation, Uses, Keynotes, and Blood Agar Footages

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Blood Agar- Introduction, Composition, Principle, Preparation Requirements, Test Procedure, Result -Interpretation, Uses, Keynotes and Blood Agar Footages

Introduction of Blood Agar Blood agar is an enriched medium for the cultivation of bacteria. Fastidious organisms, such as streptococci, do not grow well on ordinary growth media. It is a type of growth medium i.e. trypticase soy agar enriched with 5% sheep blood or blood agar base with 5-10 % sterile …

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Nutrient Agar: Introduction, Composition, Test Requirements for Preparation, Test Procedure, Colony Characteristics, Uses, and Nutrient Agar Footages

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Staphylococcus aureus golden yellow colony on nutrient agar

Introduction of Nutrient Agar Nutrient agar is a simple medium which uses to grow bacteria. It is devoid of indicators, selective agents, differential ingredients, and enriching substances therefore uses for better expression of pigmentation, biochemical test, and even serotyping. The short form of nutrient agar …

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Triple Sugar Iron (TSI) Agar Test: Introduction, Composition, Principle, Test Requirements, Procedure, Result- Interpretation, Keynotes, and TSI Test Footages

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Triple Sugar Iron (TSI) Agar Test: Introduction, Composition, Principle, Test Requirements, Procedure, Result- Interpretation, Keynotes, and TSI Test Footages

Triple Sugar Iron Agar Test The triple Sugar Iron (TSI) Agar test is a combo test and is useful for the identification of mainly gram-negative bacteria, on the basis of the following tests- Composition of Triple Sugar Iron Agar Triple Sugar Iron Agar (TSI) (Oxoid, England) …

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Satellitism Test for the Identification of Haemophilus influenzae: Introduction, Principle, Test Requirements, Procedure, Result -Interpretation, and Keynotes

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Satellitism Test for the Identification of Haemophilus influenzae- Introduction, Principle, Test Requirements, Procedure, Result -Interpretation and Keynotes

Introduction Satellitism test or satellite test is applied to identify Haemophilus influenzae. It is a fastidious bacterium and thus it needs extra ingredients like hemin (factor X) and  NAD (factor V) to grow. It uses the X factor to produce essential respiratory enzymes such as cytochromes, catalases, …

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SIM Test: Introduction, Principle, Test Requirements, Procedure, Result Interpretation, and Limitations

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SIM Test: Introduction, Principle, Test Requirements, Procedure, Result Interpretation, and Limitations

Introduction SIM stands for sulfide, Indole, and Motility and SIM medium is applied for the differentiation of gram-negative enteric bacilli. SIM test assists to isolate the bacteria on the basis of sulfide production, indole formation, and motility and thus SIM test is a combination of three …

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Bacteria-wise Antimicrobial Susceptibility Testing (AST): Introduction, List of Bacterial Panel, Antibiotics

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Bacteria wise Antimicrobial Susceptibility Testing (AST): Introduction, List of Bacterial Panel, Antibiotics

Introduction of Bacteria-wise Antimicrobial Susceptibility Testing (AST) All antimicrobial agents/ antibiotics are not applicable for all bacteria and for this reason, we should follow organism-wise antibiotics policy. As we know, antimicrobial resistance (AMR) is increasing day by day but no new antibiotic has not been …

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