Phase Contrast Microscope: Introduction, Principle, Parts, Uses, Care and Maintenance, and Keynotes

Phase Contrast Microscope: Introduction, Principle, Parts, Uses, Care and Maintenance, and Keynotes

Introduction A Phase Contrast Microscope is an optical instrument designed to enhance the visibility of transparent and unstained samples, such as living cells, by converting phase differences in light waves into brightness variations in the image. This technique was invented by Dutch physicist Frits Zernike …

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E. aerogenes: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Enterobacter aerogenes colony morphology on CLED agar of urine culture

Introduction Enterobacter aerogenes is a bacterium belonging to the Enterobacteriaceae family, which includes several other important pathogens and opportunistic bacteria. E. aerogenes is a Gram-negative, rod-shaped bacterium that is commonly found in various environments, including soil, water, and the gastrointestinal tracts of humans and animals. …

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Enteroviruses: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Enteroviruses are a group of viruses that belong to the Picornaviridae family. They are a diverse group of viruses that can cause a wide range of illnesses, from mild cold-like symptoms to more serious diseases. Enteroviruses primarily infect the gastrointestinal tract and can spread …

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MALDI-TOF Mass Spectrometry: Introduction, Principle, Handling Procedure, Application, and Keynotes

Introduction Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry, commonly abbreviated as MALDI-TOF MS, is a powerful analytical technique used in the field of mass spectrometry. It is widely employed for the identification and characterization of a diverse range of molecules, including proteins, peptides, nucleic acids, carbohydrates, …

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Pleural Fluid Culture and Sensitivity: Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

Pleural fluid for laboratory diagnosis

Introduction Pleural fluid culture and sensitivity is a diagnostic test used to identify and determine the antibiotic susceptibility of microorganisms present in the pleural fluid. The pleural fluid is a clear fluid that accumulates between the two layers of the pleura, the membrane that surrounds …

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Shewanella: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Shewanella species colony characteristics on Muller-Hinton agar (MHA)

Introduction Shewanella is a genus of Gram-negative, facultative anaerobic bacteria that belongs to the family Shewanellaceae. These bacteria are known for their remarkable versatility and adaptability to a wide range of environmental conditions. They were first discovered in 1980 by researchers studying microbial fuel cells, …

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Clostridium: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Clostridium growth on blood agar under anaerobic condition of 48 hours- inhibition of growth around metronidazole but no effect towards gentamicin

Introduction Clostridium is a genus of Gram-positive, rod-shaped, spore-forming bacteria that belongs to the phylum Firmicutes. These bacteria are anaerobic, meaning they can thrive in environments devoid of oxygen. The name “Clostridium” is derived from the Greek word “kloster,” which means spindle, referring to their …

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Moraxella catarrhalis: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Gram negative cocci in pairs (diplococci) of Moraxella catarrhalis in Gram staining of culture microscopy at a magnification

Introduction Moraxella catarrhalis is a Gram-negative, aerobic bacterium that belongs to the Moraxellaceae family. It is a non-motile, non-spore-forming bacterium, and it is typically found in the upper respiratory tract of humans as a commensal organism. Though generally considered a commensal, M. catarrhalis can also …

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