Dengue Fever Rapid Panel Test: Introduction, Test Result, Test Method, and Keynotes

Dengue Fever Rapid Panel- Test Result and Test Method

Introduction of Dengue Fever Rapid Panel Test Dengue Fever Rapid Panel Test is clear from the topic name that contains Dengue immunoglobulin M (IgM), Dengue immunoglobulin G (IgG), and Dengue nonstructural protein 1 (NS1) antigen tests. Blood is used for the assay and there is …

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Urinary Tract Infection Caused by Kluyvera intermedia: Patient History, Introduction of Pathogen, Lab Diagnosis, AST Pattern, and Keynotes

Gram Negative Rods of Kluyvera intermedia in Gram Staining of Culture

Patient History of Urinary Tract Infection Caused by Kluyvera An 80- year- old man with painful urination was requested for testing Urine Routine Examination (R/E), Complete Blood Cell (CBC) count, Thyroid stimulating hormone (TSH), Prostate-specific antigen (PSA), Liver function test (LFT), Renal function test (RFT), …

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Occult Blood and Helicobacter pylori Antigen Stool Test- Test Result, Unit, Normal Range, Test Method, and Clinical Significance

Occult Blood and Helicobacter pylori Antigen Stool Test- Test Result, Unit, Normal Range, Test Method, and Clinical Significance

Introduction of Occult Blood and Helicobacter pylori Antigen Stool Test Occult Blood and Helicobacter pylori Antigen Stool Tests are clinically important for the diagnosis of diseases. Fecal occult blood test positive is common in hookworm infestation. H. pylori antigen stool test is clear from the …

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Decarboxylase Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes

Decarboxylase Test-Positive and Negative Results Demonstration

Principle of Decarboxylase-Dihydrolase Test Lysine, ornithine, and arginine are the most common amino acids which use to detect an organism’s ability to decarboxylate or hydrolyze an amino acid in a decarboxylase medium, forming an amine that produces an alkaline pH. The basal medium for the …

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Methyl-α-D-glucopyranoside (MGP) Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes

Methyl-α-D-glucopyranoside (MGP) Test- Positive and Negative Results Demonstration

Introduction of Methyl-α-D-glucopyranoside (MGP)Test Methyl-α-D-glucopyranoside (MGP) test is effective for species identification of Enterococcus. Enterococcus gallinarum and Enterococcus casseliflavus are MGP test positive while Enterococcus faecalis and Enterococcus faecium are negative. Vancomycin-resistant Enterococcus faecium (VREF) is a serious nosocomial problem that is difficult to differentiate from  Enterococcus gallinarum and Enterococcus casseliflavus, while not important for purposes of control of …

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Indoxyl Acetate Disk Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes

Indoxyl Acetate Disk Test-Positive and Negative Results

Introduction of Indoxyl Acetate Disk Test  Indoxyl is a tryptophan product of putrefactive decomposition in the intestines of humans by a bacterial esterase. This Indoxyl Acetate Disk test uses to distinguish Campylobacter species. Positive tests are Campylobacter jejuni and Campylobacter coli whereas Campylobacter lari and Campylobacter fetus are negative. Principle of Indoxyl Acetate Disk Assay The …

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Hippurate Hydrolysis Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes

Hippurate Hydrolysis Test-Positive and Negative Results

Introduction of Hippurate Hydrolysis Test The Hippurate Hydrolysis Test is applied to determine the ability of the organism to produce hippuricase which hydrolyzes the substrate hippurate. It is useful in the identification of Streptococcus agalactiae, Campylobacter jejuni, Listeria monocytogenes, and Gardnerella vaginalis.  Principle of the Test The end products …

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LAP (Leucine Aminopeptidase) Test: Introduction, Principle, Test Requirements, Procedure, Result -Interpretation, and Limitations

 LAP (Leucine Aminopeptidase) Test-Positive and Negative Bacteria

Introduction of LAP (Leucine Aminopeptidase) Test LAP stands for leucine aminopeptidase which is an enzyme useful for definitive identification of catalase-negative, gram-positive cocci of Aerococcus and Leuconostoc (LAP test negative) from other organisms in the group like  Streptococcus, Enterococcus, Lactococcus, and Pediococcus (almost always positive). Principle of LAP test Hydrolysis of the leucine-pnaphthylamide substrate by …

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