D-Zone Test: Introduction, Principle, Procedure, Result-Interpretation, Clinical Significance, and Keynotes

D-Zone Test Positive strain of Staphylococcus aureus

Introduction The D-Zone test identifies inducible clindamycin resistance in bacteria. Typically, it involves using erythromycin and clindamycin discs on an agar plate. When placed near each other, the interaction is observed. If a D-shaped zone appears around clindamycin, resistance is confirmed. Consequently, this test helps …

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Serratia liquefaciens: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Serratia liquefaciens and Klebsiella pneumoniae growth on CLED agar of Urine Culture

Introduction Serratia liquefaciens is a species of Gram-negative, rod-shaped bacteria belonging to the genus Serratia. This bacterium is known for its diverse distribution in various environmental niches, including water, soil, plants, and human-related habitats. It has also been associated with clinical infections in humans, making …

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MALDI-TOF Mass Spectrometry: Introduction, Principle, Handling Procedure, Application, and Keynotes

Introduction Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry, commonly abbreviated as MALDI-TOF MS, is a powerful analytical technique used in the field of mass spectrometry. It is widely employed for the identification and characterization of a diverse range of molecules, including proteins, peptides, nucleic acids, carbohydrates, …

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Pus Culture and Sensitivity: Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

Introduction Pus culture and sensitivity is a diagnostic test commonly performed in medical settings to identify and analyze the microorganisms present in a wound, abscess, or other sources of pus. Pus is a thick, yellowish or greenish fluid that accumulates at the site of infection …

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Bipolar Staining: Introduction, Uses, and Keynotes

Bipolar Staining: Introduction, Uses, and Keynotes

Introduction Bipolar staining refers to a specific pattern of staining observed in certain bacteria during Gram staining, a common laboratory technique used to classify bacteria based on their cell wall characteristics. In a typical Gram stain, bacterial cells are first stained with crystal violet, followed …

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Enterococcus durans: Introduction, Classification, Morphology, Pathogenicity, Lab Diagnosis, Treatment, and Prevention

Enterococcus durans Colony Morphology on CLED Agar of Urine Culture

Introduction Enterococcus durans is a Gram-positive, facultative anaerobic bacterium that belongs to the family Enterococcaceae. It is commonly found in the gastrointestinal tract of humans and animals, as well as in soil and water. It is known for its ability to survive in harsh environments, …

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