MALDI-TOF Mass Spectrometry: Introduction, Principle, Handling Procedure, Application, and Keynotes

Introduction Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry, commonly abbreviated as MALDI-TOF MS, is a powerful analytical technique used in the field of mass spectrometry. It is widely employed for the identification and characterization of a diverse range of molecules, including proteins, peptides, nucleic acids, carbohydrates, …

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Pleural Fluid Culture and Sensitivity: Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

Pleural fluid for laboratory diagnosis

Introduction Pleural fluid culture and sensitivity is a diagnostic test used to identify and determine the antibiotic susceptibility of microorganisms present in the pleural fluid. The pleural fluid is a clear fluid that accumulates between the two layers of the pleura, the membrane that surrounds …

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Shewanella: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Shewanella species colony characteristics on Muller-Hinton agar (MHA)

Introduction Shewanella is a genus of Gram-negative, facultative anaerobic bacteria that belongs to the family Shewanellaceae. These bacteria are known for their remarkable versatility and adaptability to a wide range of environmental conditions. They were first discovered in 1980 by researchers studying microbial fuel cells, …

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Clostridium: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Clostridium growth on blood agar under anaerobic condition of 48 hours- inhibition of growth around metronidazole but no effect towards gentamicin

Introduction Clostridium is a genus of Gram-positive, rod-shaped, spore-forming bacteria that belongs to the phylum Firmicutes. These bacteria are anaerobic, meaning they can thrive in environments devoid of oxygen. The name “Clostridium” is derived from the Greek word “kloster,” which means spindle, referring to their …

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Moraxella catarrhalis: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Gram negative cocci in pairs (diplococci) of Moraxella catarrhalis in Gram staining of culture microscopy at a magnification

Introduction Moraxella catarrhalis is a Gram-negative, aerobic bacterium that belongs to the Moraxellaceae family. It is a non-motile, non-spore-forming bacterium, and it is typically found in the upper respiratory tract of humans as a commensal organism. Though generally considered a commensal, M. catarrhalis can also …

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Thorat Swab Culture and Sensitivity: Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

Pin point beta-haemolytic colony of Streptococcus pyogenes (group A streptococci) on blood agar of throat swab culture

Introduction of Thorat Swab Culture and Sensitivity Thorat swab culture and sensitivity is a medical diagnostic procedure used to identify the causative agents of infections in the throat and determine their sensitivity to various antibiotics. It is commonly performed when a patient presents with symptoms …

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Pericardial Fluid Culture and Sensitivity: Introduction, Report, Clinical Significance, Common Pathogens, and Keynotes

Introduction Pericardial fluid culture and sensitivity is a diagnostic test performed to identify and determine the antibiotic susceptibility of microorganisms present in the pericardial fluid. The pericardium is a protective membrane that surrounds the heart, and the pericardial fluid is a small amount of fluid …

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Staphylococcus saprophyticus: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Staphylococcus saprophyticus on Muller-Hinton agar (MHA) showing novobiocin resistance

Introduction Staphylococcus saprophyticus is a species of bacteria that belongs to the genus Staphylococcus. It is a Gram-positive, coagulase-negative, and facultative anaerobic bacterium. Like other staphylococci, S. saprophyticus typically appears in clusters under a microscope, which is characteristic of its name, derived from the Greek …

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