Potato Dextrose Agar (PDA): Introduction, Composition, Principle, Test Requirements, Procedure, Result-Interpretation, Colony Characteristics, Modifications, Uses, and Keynotes

A. fumigatus growth on PDA

Introduction of Potato Dextrose Agar (PDA) Potato Dextrose Agar ( PDA) is a fungal medium and its ingredients are clear from its name it contains potato ( vegetable), dextrose ( sugar), and agar ( solidifying agent). PDA  is recommended for the isolation and enumeration of fungi …

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Bile Solubility Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, and Keynotes

Bile Solubility Test- Positive and Negative

Introduction of Bile Solubility Test Bile solubility test uses for presumptive identification of Streptococcus pneumoniae. Principle of Bile Solubility Test Bile salts, specifically sodium deoxycholate and sodium taurocholate, have the capacity to selectively lyse Streptococcus pneumoniae when added to actively growing bacteria in agar or broth media. S. pneumoniae produces autolytic …

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Dengue Serotyping Real-Time PCR Test: Introduction, Principle, Test Requirement, Procedure, Result-Interpretation, and Keynotes

Positive curve of Dengue serotype-1

Introduction of Dengue Serotyping Real-Time PCR Test Dengue is a mosquito (Aedes aegypti)-borne viral disease. It is a major health threat in the tropical and sub-tropical territories of the world, particularly in urban and semi-urban areas. Approximately 50-100 million individuals have been infected with dengue …

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Enzyme-Linked Immunosorbent Assay (ELISA): Introduction, Principle, Types, Procedure, Result-Interpretation, Advantage, Disadvantage, and Interfering Factors of ELISA Test Results

Enzyme-Linked Immunosorbent Assay(ELISA) Introduction, Principle, Types, Procedure, Result-Interpretation, Advantage, Disadvantage, and Interfering Factors of ELISA Test Results

Introduction of ELISA ELISA stands for Enzyme-Linked Immunosorbent Assay. It comes under antigen and antibody reaction tests and is useful for the identification of antigens or antibodies of the following specimens serum, urine, CSF, sputum, semen, supernatant of culture, stool, etc. It is also applicable …

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RPR Test: Introduction, Principle, Procedure, Result-Interpretation, Clinical Significance, and Keynotes

RPR Test: Introduction, Principle, Procedure, Result-Interpretation, Clinical Significance, and Keynotes

Introduction of Rapid Plasma Reagin (RPR) Test RPR is a screening non-specific test for syphilis caused by a bacterium, Treponema pallidum. It has replaced the VDRL test due to having properties like no need of preparing fresh reagents daily, no need for a microscope, and …

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TPHA Test: Introduction, Principle, Procedure, Result-Interpretation, Clinical Significance, and Keynotes

TPHA Test: Introduction, Principle, Procedure, Result-Interpretation, Clinical Significance, and Keynotes

Introduction of TPHA Test TPHA test  stands for Treponema pallidum hemagglutination. This is a confirmatory test for syphilis that is caused by a bacterium, T. pallidum. TPHA test is a sensitive indirect hemagglutination test, that detects specific Treponema pallidum antibodies in serum within one hour. It uses …

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VDRL Test: Introduction, Principle, Procedure, Result-Interpretation,Limitations, and Keynotes

VDRL Test Introduction,Principle, Procedure, Result-Interpretation,Limitations, and Keynotes

Introduction of VDRL Test VDRL stands for Veneral disease research laboratory. The laboratory was renamed the Treponemal Pathogenesis and Immunology Branch of the United States Public Health Service. The Venereal Disease Research Laboratory (VDRL) test is one of two variations of micro flocculation procedures used for serological testing …

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HLA-B27 Test: Introduction, Result-Interpretation, Clinical Significance, and Keynotes

HLA-B27 Test- Introduction, Result-Interpretation, Clinical Significance, and Keynotes

Introduction of HLA-B27 Test HLA-B27 stands for Human leukocyte antigen B27. It is a protein found on the surface of our white blood cells (WBCs) and it is a blood test that identifies HLA-B27 proteins. Although most human leukocyte antigens (HLAs) protect your body from …

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