Hanging Drop Preparation: Introduction, Test Requirements, Procedure, Result-Interpretation, Keynotes, and Bacterial Motility Footages

Prepared hanging drop ready for microscopy

Introduction of Motility Test for Bacteria/ Hanging Drop Preparation Bacteria are motile due to having flagellum/flagella. On the flagella, bacteria are motile and non-motile and therefore some bacteria are motile (Vibrio cholerae, E. coli, Proteus species, etc.) whereas some others are non-motile like Klebsiella, Shigella, …

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Klebsiella oxytoca: Introduction, Identification Features, Keynotes, and Footage

Klebsiella oxytoca biochemical tests in TSI, SIM, Urea and Citrate Agar Demonstration

The genus Klebsiella is from the surname of  German-Swiss microbiologist Edwin Klebs (1834–1913). Klebsiella is a member of the family, Enterobacteriaceae and it is a Gram-negative, oxidase-negative, fimbriated, non -motile, non-sporing rod-shaped bacterium with a prominent polysaccharide-based capsule and having a size of 0.5 to 0.8 µm wide …

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Klebsiella pneumoniae: Introduction, Identification Features, Keynotes, and Klebsiella pneumoniae Footages

Klebsiella pneumoniae biochemical tests in TSI, SIM, Citrate and Urea agar

Introduction The genus Klebsiella is from the surname of  German-Swiss microbiologist Edwin Klebs (1834–1913). Klebsiella is a member of the family, Enterobacteriaceae and it is a Gram-negative, oxidase-negative, fimbriated, non -motile, non-sporing rod-shaped bacterium with a prominent polysaccharide-based capsule and having a size of 0.5 to 0.8 …

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E. coli: Introduction, Identification Features, Keynotes, and Escherichia coli Footages

Biochemical reactions of E. coli in TSI, SIM, urea and citrate agar

Introduction of E. coli Escherichia coli is a member of the family, Enterobacteriaceae and it is a Gram-negative, aerobe, and facultative anaerobic, rod-shaped bacterium. The optimal temperature for growth is 36-37°C with most strains growing over the range 18-44 °C. It is a commensal that is found …

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Serratia marcescens: Introduction, Identification Features, and Serratia Footages

Serratia marcescens colony morphology on nutrient agar, biochemical reactions and antibiogram

Introduction of Serratia marcescens Serratia is a member of the family, Enterobacteriaceae and it is a Gram-negative, facultatively anaerobic, rod-shaped lacking spore and capsule bacterium. This is a Gram-negative rod/ bacilli that thrive in moist environments. It frequently contaminates solutions and hospital equipment and the human reservoirs are …

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Hafnia alvei: Introduction, and Properties

Hafnia alvei (white mucoid) colony on Muller-Hinton Agar (MHA)

Introduction of Hafnia alvei Hafnia alvei is a members of the Enterobacteriaceae and it is inhabited in our gastrointestinal tract and also in many animal species as part of the normal microflora. It is a Gram negative rod, non capsulated and non-sporing bacterium motile with …

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Enterobacteriaceae: Introduction, Phenotypic Features, Keynotes, and Related photos

Enterobacteriaceae: Introduction, Phenotypic Features and Keynotes and Related photos

Introduction of Enterobacteriaceae The family Enterobacteriaceae was first proposed by Rahn in 1936 and it is a very large group of gram-negative bacteria that possess a number of common morphological and biochemical properties and similar DNA base compositions. The widely used systems for the classification …

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Lactose Fermenter (LF) and Non-Lactose Fermenter (NLF) Gram-Negative Bacteria: Introduction, List of LF and NLF Bacteria, and Keynotes

LF and NLF Colonies of Klebsiella and Pseudomonas on MacConkey medium

Introduction of LF and NLF Gram-Negative Bacteria on MacConkey Medium MacConkey agar/ medium is recommended for use as a selective, differential, and indicator growth medium for the isolation of gram-negative bacilli including coliform organisms and enteric pathogens, on the basis of lactose fermentation. Peptones (meat …

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