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Antimicrobial Susceptibility Testing (AST): Introduction, Principle, Test Methods, Test Requirements, Procedure, and Result Interpretation, Application, and Keynotes

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Antimicrobial Susceptibility Testing (AST): Introduction, Principle, Test Methods, Test Requirements, Procedure, and Result Interpretation, Application, and Keynotes

Introduction Antimicrobial Susceptibility Testing (AST) is a laboratory technique used to assess the effectiveness of antimicrobial agents, such as antibiotics, against specific microorganisms. The aim of AST is to determine the susceptibility or resistance of a microorganism to different antimicrobial drugs. This information is crucial …

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Tryptone Soy Broth: Introduction, Composition, Principle, Preparation, Testing Procedure, Colony Characteristics, Limitations, and Keynotes

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BD Bactec Tryptone Soy Broth bottles freshly inoculated blood specimens for culture

Introduction and Composition Tryptone Soy Broth (TSB) is a versatile and widely used liquid medium in microbiology. It is a nutrient-rich broth that supports the growth of a wide range of bacteria, making it suitable for general cultivation, enumeration, and maintenance of bacterial cultures. TSB …

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Shigella sonnei: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

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Shigella sonnei in wet mount of Tryptone soy broth microscopy

Introduction Shigella sonnei is a bacterium that is responsible for causing shigellosis, a type of bacterial infection affecting the digestive system. Shigellosis is commonly characterized by symptoms such as diarrhea, abdominal pain, fever, and occasionally, bloody stools. It is a specific serotype of the Shigella …

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E (Epsilometer) Test-Introduction, Principle, Purpose, Procedure, Result-Interpretation, Advantages, and Disadvantages

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Polymyxin B and Colistin E- Test for Acinetobacter

Introduction The E-test, also known as the Epsilometer test, is a laboratory technique used to determine the antimicrobial susceptibility of microorganisms, particularly bacteria. It is a quantitative method that provides information about the minimum inhibitory concentration (MIC) of an antimicrobial agent against a specific organism. …

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Morganella: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

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Morganella morganii Colony Morphology on CLED agar of Urine Culture

Introduction Morganella is a genus of bacteria belonging to the family Enterobacteriaceae. The genus was named after the bacteriologist John Morgan. Morganella species are gram-negative, rod-shaped bacteria that are commonly found in the environment, including soil, water, and sewage. One species of particular interest is …

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Serratia ficaria: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment,Prevention, and Keynotes

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Serratia ficaria on CLED Agar of Urine Culture

Introduction Serratia ficaria is a bacterial species that belongs to the genus Serratia. It is a Gram-negative, rod-shaped bacterium that is commonly found in various environmental niches, including soil, water, and plants. Serratia ficaria is known for its ability to produce a distinctive red pigment …

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Aeromonas sobria: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

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Aeromonas sobria colony morphology on CLED agar of Urine Culture after 4 days of incubation

Introduction Aeromonas sobria is a species of Gram-negative bacteria that belongs to the Aeromonas genus. It is a rod-shaped, facultative anaerobic bacterium that is commonly found in aquatic environments, such as freshwater and brackish water. A. sobria can cause a range of infections in humans, …

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Yersinia: Introduction, Classification Morphology, Pathogenicity, Lab Diagnosis, Treatment, and Prevention

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Yersinia enterocolitica colony morphology on CLED agar of Urine Culture

Introduction Yersinia is a genus of bacteria that includes several species, some of which are pathogenic to humans. The most well-known species within this genus are Yersinia pestis, the causative agent of the bubonic and pneumonic plague, and Y. enterocolitica, a cause of gastrointestinal infections. …

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