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Introduction of Nitrate or Nitrite Reduction Test The nitrate or nitrite reduction test uses to determine the ability of an organism to reduce nitrate to nitrite. Anaerobic metabolism requires an electron acceptor other than atmospheric oxygen. Many gram-negative bacteria use nitrate as the final electron acceptor. The …
Introduction of DNase Test for Bacteria DNase tests positive and negative bacteria as shown below image. This test is useful for the presumptive identification of Staphylococcus aureus which produces the enzyme deoxyribonuclease (DNase) from other Staphylococci which do not produce DNase. This test is also positive in the following …
Introduction of ONPG Test ONPG test is useful in differentiating members of the family Enterobacteriaceae and genera Neisseria based on b-D-galactosidase activity. ONPG stands for o-Nitrophenyl-b-D-galactopyranoside. Here we discuss on ONPG Disk Test which is simple and easy to perform. Principle The ability of an organism to ferment lactose …
Introduction of MIU Test MIU test stands for motility indole urease test and it is used for three tests among them one for motility of bacteria, second for indole formation, and third for urea hydrolyzation test of bacteria. This assay is useful for the identification …
Introduction of Laboratory Instruments and Equipment Laboratory Instruments and Equipment make laboratory work easy and fast. Here we discuss the instruments and equipment of the Department of Pathology and Laboratory Medicine that includes Clinical Haematology, Clinical Biochemistry, Histopathology and Cytology, Clinical Microbiology and Immunology, Blood …
Introduction of Culture Media Culture media are required to grow the organisms and they are generally from clinical specimens to identify the causative agent. Common Ingredients of Culture Media Common Ingredients of Culture Media and their basic constituents are as follows- Water: It is the …
Introduction of Aeromonas hydrophila Aeromonas hydrophila is a gram-negative, facultative anaerobe, rod-shaped motile(due to single polar flagellum) bacterium without capsule and spore having a size from 0.3-1.0 µm wide by 1.0-3.5 µm long. It is generally found in the aquatic environment. Its presence can not …
Introduction of Enterobacter cloacae Enterobacter cloacae is a facultative anaerobic Gram-negative bacterium of size 0.3-0.6 x 0.8-2.0 μm. It is lacking capsule and spore and it is motile due to flagella which is a member of the Enterobacteriaceae family. E. cloacae is the normal gut …