Methyl Red Test: Introduction, Principle, Test Requirements, Test Procedure, Result-Interpretation, Positive and Negative Bacteria, and Limitations

Methyl Red Test-Positive and Negative Test Results

Introduction of Methyl Red Test The Methyl Red test is for enteric gram-negative rods, as part of identification to species level. Its short name is MR test. Principle of Methyl Red Test The methyl red (MR) test uses to determine if an organism is able …

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Auramine-Phenol Fluorochrome Staining: Introduction, Principle, Test Requirements, Test Procedure, Result- Interpretation, Limitations, and Keynotes

Acid fast bacilli (AFB) of Mycobacterium tuberculosis in Auramine-phenol fluorochrome Staining

Introduction of Auramine-Phenol stain Auramine-Phenol is a fluorochrome stain used to visualize acid-fast structures of various microorganisms, especially Mycobacterium tuberculosis and in modified form for Mycobacterium leprae, Nocardia species, Cryptosporidium parvum, Cyclospora cayetanensis , Isospora belli, and fungal spores. Ziehl-Neelsen (hot), and Kinyoun (cold) are still widely used methods to detect acid-fast structures …

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ADA (Adenosine Deaminase) Test: Introduction, Principle, Test Requirements, Procedure, Normal Range, and Clinical Significance

ADA (Adenosine Deaminase) Test Reading in Spectrophotometer

Introduction of ADA (Adenosine Deaminase) Test ADA (Adenosine Deaminase) Test is very useful for suspicious cases of tuberculosis, increased levels of ADA could facilitate diagnosis. ADA is an enzyme that primarily functions in our body in the development and maintenance of the immune system that catalyzes the …

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Vibrio cholerae Serotyping: Introduction, Principle, Test Requirements, Test Procedure, Result- Interpretation and Keynotes

Vibrio cholerae Serotyping Procedure

Introduction of Vibrio cholerae Serotyping Vibrio Serotyping helps to differentiate serotypes of Vibrio. Serotypes refer to separate groups within a species of microorganisms that all share a similar property. More specifically, each serotype has the same number of antigens on its surfaces. For example, V.cholerae O1 serovar Inaba, V. cholerae O1 serovar …

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Haemophilus Serotyping: Introduction, Principle, Test Requirements, Test Procedure, Result-Interpretation, Limitations, and Keynotes

Use of bacitracin 10 U on chocolate agar for screening Haemophilus of sputum culture

Introduction of Haemophilus Serotyping Haemophilus Serotyping helps to differentiate serotypes of Haemophilus influenzae. Serotypes refer to separate groups within a species of microorganisms that all share a similar property. More specifically, each serotype has the same number of antigens on its surfaces. For example, encapsulated strains of Haemophilus influenzae may …

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Shigella Serotyping: Introduction, Principle, Test Requirements, Test Procedure, Result-Interpretation, and Keynotes

Shigella polyvalent A, B and C2 for Shigella dysenteriae, Shigella flexneri, and Shigella boydii

Introduction of Shigella Serotyping Shigella Serotyping helps to differentiate serotypes of Shigella. Serotypes refer to separate groups within a species of microorganisms that all share a similar property. More specifically, each serotype has the same number of antigens on its surfaces. For example, Shigella flexneri serotype 1, 2, 2a, S. sonnei, and S. …

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ABO and Rh Typing: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Applications, and Keynotes

ABO and Rh Typing-Slide Method

Introduction of ABO and Rh Typing ABO blood grouping and Rh typing are the most common blood group systems. There are nearly 300 blood group systems so far discovered. Other than ABO and  Rh are MNS, Lutheran, Kell, Duffy, etc. Karl Landsteiner discovered ABO ( …

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GeneXpert Test For Tuberculosis: Introduction, Principle, Test Requirements, Test Procedure, Result -Interpretation, Advantages, Disadvantage, and Keynotes

GeneXpert Test For Tuberculosis-Mycobacterium tuberculosis (MTB) Detected High

Introduction of GeneXpert Test GeneXpert test is a molecular test for tuberculosis diagnosis by detecting the presence of  Mycobacterium tuberculosis (bacteria), as well as testing for resistance to the drug rifampicin. Principle of GeneXpert Test GeneXpert test is a highly sensitive and specific test for tuberculosis(TB) diagnosis that detects DNA sequences …

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