Test Requirements Archives - Page 5 of 6

Bile Solubility Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, and Keynotes

January 13, 2023January 13, 2023 by Medical Lab Notes

Bile Solubility Test- Positive and Negative

Introduction of Bile Solubility Test Bile solubility test uses for presumptive identification of Streptococcus pneumoniae. Principle of Bile Solubility Test Bile salts, specifically sodium deoxycholate and sodium taurocholate, have the capacity to selectively lyse Streptococcus pneumoniae when added to actively growing bacteria in agar or broth media. S. pneumoniae produces autolytic …

Oxidation-Fermentation (OF) Test: Introduction, Composition of the medium, Test Requirements, Principle, Procedure, Result -Interpretation, and Limitations

January 13, 2023January 6, 2023 by Medical Lab Notes

Fermentation Test Positive and Negative Results of Bacteria

Oxidation-Fermentation (OF) Test Introduction Hugh and Leifson developed an Oxidation-Fermentation (OF) basal medium in 1953. It is a recommended medium for the detection of oxidation or fermentation of carbohydrates by bacteria. This basal medium is useful to aid in the identification of gram-negative bacteria on …

Nitrate or Nitrite Reduction Test: Introduction, Composition of Medium, Principle, Test Requirements, Procedure, Result -Interpretation, and Limitations

January 13, 2023January 5, 2023 by Medical Lab Notes

Introduction of Nitrate or Nitrite Reduction Test The nitrate or nitrite reduction test uses to determine the ability of an organism to reduce nitrate to nitrite. Anaerobic metabolism requires an electron acceptor other than atmospheric oxygen. Many gram-negative bacteria use nitrate as the final electron acceptor. The …

Culture Media: Introduction, Common Ingredients, Types, Preparation, and Use of Common Culture Medium, Keynotes, and Related Images

January 13, 2023November 23, 2022 by Medical Lab Notes

Culture media with variety of microbes-Micrococcus growth on nutrient agar (upper left), Lactose fermenting(Lf) and non-lactose fermenting (NLF) colonies of Gram negative bacteria colonies on MacConkey medium, Hameophilus influenzae colony morphology on chocolate agar(lower left), beta-hemolytic colonies of Staphylococcus aureus( middle), and Pseudomonas aeruginosa antibiogram pattern on Muller-Hinton agar(MHA)

Introduction of Culture Media Culture media are required to grow the organisms and they are generally from clinical specimens to identify the causative agent. Common Ingredients of Culture Media Common Ingredients of Culture Media and their basic constituents are as follows- Water: It is the …

Dengue Serotyping Real-Time PCR Test: Introduction, Principle, Test Requirement, Procedure, Result-Interpretation, and Keynotes

November 26, 2022October 26, 2022 by Medical Lab Notes

Introduction of Dengue Serotyping Real-Time PCR Test Dengue is a mosquito (Aedes aegypti)-borne viral disease. It is a major health threat in the tropical and sub-tropical territories of the world, particularly in urban and semi-urban areas. Approximately 50-100 million individuals have been infected with dengue …

Hematoxylin and Eosin (H&E) Stain: Introduction, Principle, Test Requirements, Procedure, Result- Interpretation, and Keynotes

November 26, 2022October 19, 2022 by Medical Lab Notes

Introduction of Hematoxylin and Eosin Stain Hematoxylin and eosin stain is abbreviated as H&E stain. It is one of the principal tissue stains used in histopathology to find out cancer from a suspected biopsy. The stain was first introduced by A. Wissowzky in 1876. The cell nuclei …

Dengue Rapid Test for IgG and IgM Detection: Introduction, Principle, Test Requirements, Procedure, Result Interpretation, and Limitations

November 29, 2022September 6, 2022 by Medical Lab Notes

Introduction of Dengue IgG and IgM Rapid Test Dengue rapid test (RDT) uses for the detection of immunoglobulin G(IgG) and immunoglobulin M (IgM) where IgM is responsible for the past while IgG is for present infection as shown above picture. Dengue is a mosquito-borne viral …

Indole test: Introduction, Principle, Test Requirements, Procedure, Result -Interpretation, and Keynotes

December 16, 2022August 18, 2022 by Medical Lab Notes

Indole Production test This indole test is useful to identify the bacteria on the basis of indole production. The list of indole-positive organisms is- Principle of Indole test The ability of an organism to split Indole from the amino acid tryptophan is due to the presence of …