Lowenstein-Jensen (LJ) Medium: Introduction, Principle Composition, Preparation, Procedure, Result- Interpretation, Limitation, and Keynotes

Lowenstein-Jensen (LJ) Medium: Introduction, Principle Composition, Preparation, Procedure, Result- Interpretation, Limitation, and Keynotes

Introduction of Lowenstein-Jensen (LJ) Medium Lowenstein-Jensen (LJ) Medium is named after the surnames of  Austrian pathologist Ernst Lowenstein (1878–1950) and the Danish chemist Kai Arne Jensen (1908–1992). The etiological agent of tuberculosis is Mycobacterium tuberculosis. It requires aerobic conditions and a protein-enriched medium for culture. The short …

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Phenylalanine Deaminase (PDA) Test: Introduction, Principle, Test Requirements, Procedure, Result- Interpretation, Keynotes, and Limitations

Phenylalanine Deaminase(PDA) Test- Introduction, Principle, Test Requirements, Procedure, Result- Interpretation, Keynotes, and Limitations

Phenylalanine Deaminase (PDA) Test PDA stands for phenylalanine deaminase and PDA tests positive and negative are as shown below footage. It is used in the differentiation of gram-negative enteric bacilli based on the ability of the microorganisms to produce phenyl pyruvic acid by oxidative deamination. The deamination of …

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Hospitals in Kathmandu: Introduction, List of Hospitals, Locations, Description, Links, and Keynotes

Hospitals in Kathmandu: Introduction, List of Hospitals, Locations, Description, Links, and Keynotes

Introduction of Hospitals in Kathmandu Kathmandu is made up of three districts and they are Kathmandu, Lalitpur, and Bhaktapur, together with expanding an area of 899 square kilometers. It is the largest and busiest city in Nepal and thus has numerous reputed health care and …

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Cholera: Introduction, Causative Agent, Pathogenicity, Symptoms, Lab Diagnosis, Treatment, Prevention, and Keynotes

Cholera: Introduction, Causative Agent, Pathogenicity, Symptoms, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Cholera is an infectious disease that is caused by the Gram-negative bacterium, Vibrio cholerae. It is responsible for severe watery diarrhea, which can lead to dehydration and even death if untreated. Cholera occurs due to eating food or drinking water contaminated V. cholerae. 13 …

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Health Laboratory Establishment and Operation: Introduction, Space, Equipment, and Human Resource

Health Laboratory Establishment and Operation

Introduction of Health Laboratory Establishment and Operation As we know, ‘Laboratory Diagnosis’ is the third eye of doctors/clinicians that confirms the cause of pathology/ disease. It highlights the importance of Health Laboratory Establishment and Operation for better service. Here, we discuss in brief the national …

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Microbiology and Serology Laboratory: Introduction, List of Tests, Specimen,Testing Method, and Keynotes

Microbiology and Serology Laboratory: Introduction, List of Tests, Specimen,Testing Method, and Keynotes

Introduction of Microbiology and Serology Laboratory Here we are discussing not general microbiology but medical/clinical microbiology it is a discipline that encloses a wide range of testing methodologies, and it is complex in terms of organisms (bacteria, viruses, fungi, parasites) and methods used to isolate …

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Histopathology and Cytopathology Laboratory: Introduction, List of Tests, Specimen, Testing Method, and Keynotes

Histopathology and Cytopathology Laboratory: Introduction, List of Tests, Specimen, Testing Method, and Keynotes

Introduction of Histopathology and Cytopathology Laboratory Histopathology/histology is the branch of medical science that involves the examination of sampled whole tissues under a microscope. The size of the histopathological specimens is of three types, small, medium, and large. An autopsy is also called a post-mortem …

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MacConkey Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses, and Keynotes

MacConkey Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses, and Keynotes

Introduction of MacConkey Agar MacConkey agar (MAC) uses for the culture of gram-negative bacteria and therefore Enterobacteriaceae belonging bacteria grow well on this medium and coliforms also enjoy this medium. MAC is a modification of Neutral Red Bile Salt Agar developed by MacConkey. It was one of the …

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