Basic Microbiology Archives - Page 57 of 74

L-pyrrolidinyl-β-naphthylamide (PYR) Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretations, and Limitations

January 14, 2023January 14, 2023 by Medical Lab Notes

L-pyrrolidinyl-β-naphthylamide (PYR) Test-Positive

Introduction of L-pyrrolidinyl-β-naphthylamide (PYR) Test L-pyrrolidinyl-β-naphthylamide (PYR) test uses to identify the capability of organisms utilizing substrate, PYR by L-pyrroglutamyl amino-peptidase. Application of this test are as follows: Principle of L-pyrrolidinyl-β-naphthylamide (PYR) Test L-pyrrolidinyl-β-naphthylamide (PYR) acts as a substrate for the detection of the enzyme …

API Test for Bacteria: Introduction, Principle, Test Requirements, Test Procedure, Result- Interpretation, Advantages, and Keynotes

January 13, 2023January 13, 2023 by Medical Lab Notes

Introduction of API Test for Bacteria API test for bacteria as shown above picture is a 20-jumbo tests kit for a biochemical panel for the identification and differentiation of members of the family Enterobacteriaceae. API stands for Analytical Profile Index. It is an API 20E Test kit …

Bile Solubility Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, and Keynotes

January 13, 2023January 13, 2023 by Medical Lab Notes

Bile Solubility Test- Positive and Negative

Introduction of Bile Solubility Test Bile solubility test uses for presumptive identification of Streptococcus pneumoniae. Principle of Bile Solubility Test Bile salts, specifically sodium deoxycholate and sodium taurocholate, have the capacity to selectively lyse Streptococcus pneumoniae when added to actively growing bacteria in agar or broth media. S. pneumoniae produces autolytic …

Malonate Test: Introduction, Composition of Medium, Principle, Procedure, Uses, Result-Interpretation, and Limitations

January 13, 2023January 13, 2023 by Medical Lab Notes

Malonate Test Positive and Negative bacteria

Introduction of Malonate Test Malonate test uses to check the ability of the organism to utilize malonate as the sole source of carbon for energy. To differentiate bacteria on the basis of a malonate utilization test. It is a colorful test to check the ability of …

Optochin Susceptibility Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, and Keynotes

January 13, 2023January 12, 2023 by Medical Lab Notes

Optochin Susceptibility Test Optochin Susceptibility Test uses for presumptive identification of Streptococcus pneumoniae from viridans streptococci. Principle of Optochin Susceptibility Test Ethylhydrocupreine hydrochloride (Optochin), a quinine derivative, selectively inhibits the growth of S. pneumoniae at a very low concentration (5ug/ml). Optochin may also inhibit other viridians streptococci, but only …

CAMP Test: Introduction, Principle, Test Requirements, Procedure, Result-Interpretation,and Keynotes

January 13, 2023January 12, 2023 by Medical Lab Notes

Introduction of CAMP Test The CAMP test uses to identify preemptively Streptococcus agalactiae. It was first described in 1944 by Christie, Atkins, and Munch-Petersen, and the CAMP test is an acronym for their names. Principle of CAMP Test The beta lysin produced by Staphylococcus aureus is sphingomyelinase in nature, …

Acetate Utilization Test: Introduction, Composition, Principle, Test Requirements, Test Procedure, Result and Interpretation, and Keynotes

January 13, 2023January 12, 2023 by Medical Lab Notes

Acetate Utilization Test-Negative (left) and Positive (right side)

Introduction of Acetate Utilization Test Acetate Utilization Test ueses to determine the ability of an organism to use acetate as the sole source of carbon same as in the citrate utilization test for Enterobacteriaceae. It is the recommended assay for the differentiation of Shigella species from Escherichia coli. Composition of …

Corn Meal Agar (CMA): Introduction, Composition, Principle, Test Requirements, Test Procedure, Result-Interpretation, Colony Characteristics, Uses, and Keynotes

January 13, 2023January 6, 2023 by Medical Lab Notes

Colony Morphology of Aspergillus flavus on cornmeal agar (CMA) demonstration

Introduction of Corn Meal Agar (CMA) Corn Meal Agar (CMA) is a well-established fungal medium that is a suitable substrate for chlamydospore production by Candida albicans and the maintenance of fungal stock cultures. This is a very simple formulation containing only cornmeal infusion and agar. The addition of glucose …