Shigella flexneri: Introduction, Identification Features, Keynotes, and Shigella Footages

Shigella boydii colony morphology on XLD agar

Introduction of Shigella flexneri Shigella are Gram-negative bacilli measuring about 1-3 µm X 0.5 µm. They are non-motile, non-encapsulated, non-sporing, and non-acid-fast bacteria belonging to the family Enterobacteriaceae. They cause an infectious disease called Shigellosis. There are four species or serogroups based on biochemical and …

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Citrobacter koseri: Introduction, Identification Features, and Keynotes

Citrobacter koseri growth on CLED agar

Introduction of Citrobacter koseri Citrobacter koseri is previously known as Citrobacter diversus. It is a facultative anaerobe of 1.0 × 2.0–6.0 μm in size, motile, non-capsulated, and gram-negative bacillus of the family Enterobacteriaceae. Citrobacter koseri acts as etiological agents of various pathological conditions like urinary tract infections (UTIs), …

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Salmonella Paratyphi: Introduction, Identification Features, Keynotes, and Salmonella Footages

Salmonella Serovar Paratyphi growth on Nutrient agar, Blood agar, MacConkey medium, BHI broth and Biochemical reactions in TSI, SIM, citrate and urea agar

Introduction Salmonella Paratyphi (serovar) belongs to Enterobacteriaceae and thus is a Gram-negative bacterium causing mild gastroenteritis to a life-threatening bloodstream infection. It causes paratyphoid fever and it is restricted to humans. Proper sanitation and hygiene may decrease its infection chance. Travelers must be cautious during …

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Proteus vulgaris: Introduction, Identification Features, Keynotes, and Proteus Footages

Dienes phenomenon of Proteus vulgaris strains

The genus Proteus was discovered in 1885 by Hauser and it is also named after a Greek god.  Proteus is a member of the family, Enterobacteriaceae and it is a Gram-negative, oxidase-negative, fimbriated, motile, non-sporing rod-shaped bacterium without capsule and having a size of 0.4–0.8 μm in diameter and …

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Indole test: Introduction, Principle, Test Requirements, Procedure, Result -Interpretation, and Keynotes

Indole test: Introduction, Principle, Test Requirements, Procedure, Result -Interpretation, and Keynotes

Indole Production test This indole test is useful to identify the bacteria on the basis of indole production. The list of indole-positive organisms is- Principle of Indole test The ability of an organism to split Indole from the amino acid tryptophan is due to the presence of …

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Staphylococcus aureus: Introduction, Pathogenicity, Lab Diagnosis, Treatment, and Keynotes

Staphylococcus aureus: Introduction, Pathogenicity, Lab Diagnosis, Treatment, and Keynotes

Introduction of Staphylococcus aureus Scientific classification of Staphylococcus aureus is given as: Domain Bacteria Phylum Firmicutes Class Bacilli Order Bacillales Family Staphylococceae Genus Staphylococcus Species aureus Table: Biological Classification of Staphylococcus aureus Staphyle means bunch and Kokko mean berry. Staphylococcus aureus was isolated by Pasteur (1880) from a pus sample. …

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Lowenstein-Jensen (LJ) Medium: Introduction, Principle Composition, Preparation, Procedure, Result- Interpretation, Limitation, and Keynotes

Lowenstein-Jensen (LJ) Medium: Introduction, Principle Composition, Preparation, Procedure, Result- Interpretation, Limitation, and Keynotes

Introduction of Lowenstein-Jensen (LJ) Medium Lowenstein-Jensen (LJ) Medium is named after the surnames of  Austrian pathologist Ernst Lowenstein (1878–1950) and the Danish chemist Kai Arne Jensen (1908–1992). The etiological agent of tuberculosis is Mycobacterium tuberculosis. It requires aerobic conditions and a protein-enriched medium for culture. The short …

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Phenylalanine Deaminase (PDA) Test: Introduction, Principle, Test Requirements, Procedure, Result- Interpretation, Keynotes, and Limitations

Phenylalanine Deaminase(PDA) Test- Introduction, Principle, Test Requirements, Procedure, Result- Interpretation, Keynotes, and Limitations

Phenylalanine Deaminase (PDA) Test PDA stands for phenylalanine deaminase and PDA tests positive and negative are as shown below footage. It is used in the differentiation of gram-negative enteric bacilli based on the ability of the microorganisms to produce phenyl pyruvic acid by oxidative deamination. The deamination of …

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