Multiplex PCR assay-Directly on Respiratory samples:Introduction, Principle, Clinical Significance, and Keynotes

Introduction Respiratory tract infections are caused by a wide range of pathogens, including viruses, bacteria, and fungi. Conventional diagnostic methods such as culture, microscopy, and antigen detection are time-consuming and often lack sensitivity. Multiplex polymerase chain reaction (PCR) assays, applied directly to respiratory samples (sputum, …

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COVID-19 Antigen Test- Introduction, Principle, Test Requirements, Procedure, Result Interpretation, Clinical Significance and Disadvantage

COVID-19 Antigen Test-Positive

Introduction of COVID-19 Antigen Test SARS-CoV-2 antigen test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is used as a screening test on asymptomatic persons to detect or exclude Coronavirus Disease 2019 (COVID-19) or to determine whether a person who previously was diagnosed with COVID-19 …

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Biosafety Cabinet (BSC)-Introduction, Purpose and Function,  Working Mechanism, Types, and Application

Biosafety Cabinet (BSC)-Introduction, Purpose and Function,  Working Mechanism, Types, and Application

Introduction of Biosafety Cabinet (BSC) A biosafety cabinet (BSC) is a specialized enclosed, ventilated laboratory workspace that is designed for safely handling materials contaminated with pathogens or toxins. It protects the operator, the product being worked on, and the environment. BSC uses high-efficiency particulate air (HEPA)  filters to …

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Influenza A and Influenza B Rapid Test: Introduction, Principle, Procedure, Result-Interpretation, Clinical Significance and Keynotes

Influenza A and Influenza B Rapid Test-Negative

Introduction This test rapidly detects influenza A and B antigens. It employs a chromatographic assay format. Moreover, it is simple to perform. Principle The test uses immunochromatography. It employs specific antibodies to bind viral antigens. Furthermore, it uses color changes for detection. Thus, it yields …

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Top 10 Viral Pathogens- List with Short Descriptions, and Footage

Western Blot- Positive for Human Immunodeficiency Virus (HIV)

List of Top 10 Viral Pathogens Short Description of Top 10 Viral Pathogens 1. Influenza Virus (Flu Virus) 2. Human Immunodeficiency Virus (HIV) 3. Hepatitis B Virus (HBV) 4. Hepatitis C Virus (HCV) 5. SARS-CoV-2 (COVID-19 Virus) 6. Dengue Virus 7. Human Papillomavirus (HPV) 8. …

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Bacteriophages-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Bacteriophages, often called phages for short, are viruses that specifically infect and replicate within bacteria. They are among the most abundant and diverse biological entities on Earth and play a crucial role in regulating bacterial populations in various ecosystems. Here’s an introduction to bacteriophages: …

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Epstein-Barr virus (EBV): Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Epstein-Barr virus (EBV), also known as human herpesvirus 4 (HHV-4), is a member of the herpesvirus family and is one of the most common human viruses. EBV is known for its ability to establish lifelong infections in humans, primarily in B lymphocytes (a type …

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HBsAg ELISA-Introduction, Test Results, Test Methods, Clinical Significance, and Keynotes

HBsAg ELISA-Introduction, Test Results, Test Methods, Clinical Significance, and Keynotes

Introduction Hepatitis B is a viral infection that can lead to serious liver-related diseases, including cirrhosis and hepatocellular carcinoma. Early detection and diagnosis of Hepatitis B are essential for effective management and prevention of transmission. HBsAg ELISA, an acronym for Hepatitis B Surface Antigen Enzyme-Linked …

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