Multiplex PCR assay-Directly on Respiratory samples:Introduction, Principle, Clinical Significance, and Keynotes

Introduction Respiratory tract infections are caused by a wide range of pathogens, including viruses, bacteria, and fungi. Conventional diagnostic methods such as culture, microscopy, and antigen detection are time-consuming and often lack sensitivity. Multiplex polymerase chain reaction (PCR) assays, applied directly to respiratory samples (sputum, …

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COVID-19 Antigen Test- Introduction, Principle, Test Requirements, Procedure, Result Interpretation, Clinical Significance and Disadvantage

COVID-19 Antigen Test-Positive

Introduction of COVID-19 Antigen Test SARS-CoV-2 antigen test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is used as a screening test on asymptomatic persons to detect or exclude Coronavirus Disease 2019 (COVID-19) or to determine whether a person who previously was diagnosed with COVID-19 …

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Biosafety Cabinet (BSC)-Introduction, Purpose and Function,  Working Mechanism, Types, and Application

Biosafety Cabinet (BSC)-Introduction, Purpose and Function,  Working Mechanism, Types, and Application

Introduction of Biosafety Cabinet (BSC) A biosafety cabinet (BSC) is a specialized enclosed, ventilated laboratory workspace that is designed for safely handling materials contaminated with pathogens or toxins. It protects the operator, the product being worked on, and the environment. BSC uses high-efficiency particulate air (HEPA)  filters to …

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Geobacillus stearothermophilus: The Gold Standard for Autoclave Sterilization

Geobacillus stearothermophilus (formerly Bacillus stearothermophilus) is the most commonly used biological indicator (BI) organism for monitoring steam sterilization (autoclaving)

Introduction Geobacillus stearothermophilus (formerly Bacillus stearothermophilus) is the most commonly used biological indicator (BI) organism for monitoring steam sterilization (autoclaving). Below are some key points about why and how it is used for quality control (QC) checks: Why Geobacillus stearothermophilus? How Biological Indicators Work Interpreting …

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Top 10 Viral Pathogens- List with Short Descriptions, and Footage

Western Blot- Positive for Human Immunodeficiency Virus (HIV)

List of Top 10 Viral Pathogens Short Description of Top 10 Viral Pathogens 1. Influenza Virus (Flu Virus) 2. Human Immunodeficiency Virus (HIV) 3. Hepatitis B Virus (HBV) 4. Hepatitis C Virus (HCV) 5. SARS-CoV-2 (COVID-19 Virus) 6. Dengue Virus 7. Human Papillomavirus (HPV) 8. …

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Human Metapneumovirus (HMPV) MCQs Quiz-A simple set of Questionnaire for Examination

Human Metapneumovirus (HMPV) MCQs Quiz Here is a set of 10 Multiple-Choice Questions (MCQs) with solutions regarding Human Metapneumovirus (HMPV): Answer Sheet with Explanation for Human Metapneumovirus (HMPV) MCQs Quiz

HMPV-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

HMPV-Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Introduction Human Metapneumovirus (HMPV) is a respiratory pathogen that affects children and older adults. Additionally, it belongs to the Paramyxoviridae family and emerged in 2001. Moreover, it shares genetic similarities with Avian Metapneumovirus, suggesting a possible zoonotic origin. HMPV typically causes respiratory symptoms like coughing, wheezing, …

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Presence of Numerous Pus Cells Without Detectable Microbes in Gram-Stained Sputum: Introduction, Diagnostic Implications, and Clinical Considerations

Presence of Numerous Pus Cells Without Detectable Microbes in Gram-Stained Sputum: Introduction, Diagnostic Implications, and Clinical Considerations

Introduction Gram staining is a routine diagnostic tool for respiratory infections. It reveals bacteria in sputum samples. Occasionally, Gram staining shows numerous pus cells but no microbes. This finding raises several clinical questions and concerns. Pus cells indicate an inflammatory response. They suggest a possible …

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