Genomic Analysis

Fungal Identification by NGS: Introduction, Principle, Test Methods, Test Procedure, Result-Interpretation, Clinical Significance, and Keynotes

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PCR products of fungal DNA from pure culture after amplification with primers

Introduction Fungal identification by NGS (Next-Generation Sequencing) uses advanced DNA sequencing. Moreover, it rapidly detects diverse fungal species. It enhances diagnostic accuracy. Additionally, it supports personalized treatment decisions. Principle NGS sequences fungal DNA directly. Consequently, it generates millions of short DNA reads. Then, bioinformatics tools …

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Next-Generation Sequencing (NGS) in Clinical Mycology-Introduction, Application, and Keynote

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Next-Generation Sequencing (NGS) in Clinical Mycology-Introduction, Application, and Keynote

Introduction Next-generation sequencing (NGS) in Clinical Mycology is revolutionizing the diagnosis, typing, and antifungal resistance profiling of fungal pathogens. Application Here’s an overview of its applications: Candida spp.: Aspergillus spp.: CYP51A: Azole resistance due to mutations in ergosterol synthesis. Advantages of NGS in Clinical Mycology …

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Multilocus sequence typing (MLST)-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

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Introduction Multilocus Sequence Typing (MLST) is a molecular typing technique used in microbiology and genetics to classify and differentiate bacterial strains based on their genetic diversity. MLST is particularly valuable for studying the epidemiology and evolution of bacterial pathogens. Here is an introduction to the …

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Pulsed-field gel electrophoresis (PFGE)-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

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Pulsed-field gel electrophoresis (PFGE)-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

Introduction Pulsed-field gel electrophoresis (PFGE) is a powerful laboratory technique used in molecular biology and genetics to separate and analyze large DNA molecules, such as genomic DNA, by their size. PFGE is particularly valuable when studying organisms with complex genomes, such as bacteria, yeast, and …

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