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Antimicrobial Susceptibility Testing (AST): Introduction, Principle, Test Methods, Test Requirements, Procedure, and Result Interpretation, Application, and Keynotes

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Antimicrobial Susceptibility Testing (AST): Introduction, Principle, Test Methods, Test Requirements, Procedure, and Result Interpretation, Application, and Keynotes

Introduction Antimicrobial Susceptibility Testing (AST) is a laboratory technique used to assess the effectiveness of antimicrobial agents, such as antibiotics, against specific microorganisms. The aim of AST is to determine the susceptibility or resistance of a microorganism to different antimicrobial drugs. This information is crucial …

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Tryptone Soy Broth: Introduction, Composition, Principle, Preparation, Testing Procedure, Colony Characteristics, Limitations, and Keynotes

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BD Bactec Tryptone Soy Broth bottles freshly inoculated blood specimens for culture

Introduction and Composition Tryptone Soy Broth (TSB) is a versatile and widely used liquid medium in microbiology. It is a nutrient-rich broth that supports the growth of a wide range of bacteria, making it suitable for general cultivation, enumeration, and maintenance of bacterial cultures. TSB …

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Shigella sonnei: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

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Shigella sonnei in wet mount of Tryptone soy broth microscopy

Introduction Shigella sonnei is a bacterium that is responsible for causing shigellosis, a type of bacterial infection affecting the digestive system. Shigellosis is commonly characterized by symptoms such as diarrhea, abdominal pain, fever, and occasionally, bloody stools. It is a specific serotype of the Shigella …

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E (Epsilometer) Test-Introduction, Principle, Purpose, Procedure, Result-Interpretation, Advantages, and Disadvantages

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Polymyxin B and Colistin E- Test for Acinetobacter

Introduction The E-test, also known as the Epsilometer test, is a laboratory technique used to determine the antimicrobial susceptibility of microorganisms, particularly bacteria. It is a quantitative method that provides information about the minimum inhibitory concentration (MIC) of an antimicrobial agent against a specific organism. …

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Citrobacter freundii: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

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Citrobacter freundii growth on CLED agar of Urine Culture

Introduction Citrobacter freundii is a Gram-negative bacterium belonging to the Enterobacteriaceae family. It is commonly found in the environment, including soil, water, and the intestinal tracts of humans and animals. Citrobacter freundii is an opportunistic pathogen, meaning it can cause infections in individuals with weakened …

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Morganella: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

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Morganella morganii Colony Morphology on CLED agar of Urine Culture

Introduction Morganella is a genus of bacteria belonging to the family Enterobacteriaceae. The genus was named after the bacteriologist John Morgan. Morganella species are gram-negative, rod-shaped bacteria that are commonly found in the environment, including soil, water, and sewage. One species of particular interest is …

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Serratia ficaria: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment,Prevention, and Keynotes

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Serratia ficaria on CLED Agar of Urine Culture

Introduction Serratia ficaria is a bacterial species that belongs to the genus Serratia. It is a Gram-negative, rod-shaped bacterium that is commonly found in various environmental niches, including soil, water, and plants. Serratia ficaria is known for its ability to produce a distinctive red pigment …

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Aeromonas sobria: Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

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Aeromonas sobria colony morphology on CLED agar of Urine Culture after 4 days of incubation

Introduction Aeromonas sobria is a species of Gram-negative bacteria that belongs to the Aeromonas genus. It is a rod-shaped, facultative anaerobic bacterium that is commonly found in aquatic environments, such as freshwater and brackish water. A. sobria can cause a range of infections in humans, …

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