Fungal Elements in KOH Mount of Pleural Fluid Microscopy-Introduction, Possible Fungi Observed in Pleural Fluid KOH Mount, Applications, and Keynotes

Fungal Elements in KOH Mount of Pleural Fluid Microscopy-Introduction, Possible Fungi Observed in Pleural Fluid KOH Mount, Applications, and Keynotes

Introduction KOH Mount of Pleural Fluid is a rapid, direct microscopic test used to detect fungal elements in suspected pleural infections. A 10–20% Potassium Hydroxide (KOH) solution clears cellular debris, fibrin, and protein content in pleural fluid while preserving chitin-rich fungal structures such as hyphae, …

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Sterility Testing (GMP) for Mycology Lab:Introduction, Application, and Keynotes

Fungal growth on a stored normal saline plastic bottle

Introduction Sterility testing in a GMP-compliant mycology laboratory is a critical quality assurance process used to confirm that pharmaceutical products, culture media, reagents, water systems, and laboratory environments are free from viable microorganisms, including fungi and yeasts.It follows regulatory guidelines defined by WHO, EU-GMP, USP …

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Fungal Elements in KOH Mount of Bile Microscopy-Introduction, Fungal Elements Observed in Bile KOH Mount, Applications, and Keynotes

Bile sample before centrifugation for KOH mount and fungal culture

Introduction KOH Mount of Bile is a direct microscopic examination technique used to detect fungal elements in bile fluid collected during ERCP, biliary drainage, cholecystectomy, or liver procedures.Because bile contains mucus, epithelial debris, fats, and pigments, a 10–20% Potassium Hydroxide (KOH) solution is used to …

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Fungal Elements in KOH Mount of Sputum Microscopy-Introduction, Fungal Elements Observed in Sputum KOH Mount, Applications, and Keynotes

Fungal Elements in KOH Mount of Sputum Microscopy at a magnification of 1600X

Introduction KOH mount of sputum is a rapid, direct microscopic technique used to detect fungal elements in the respiratory tract. A 10–20% Potassium Hydroxide (KOH) solution digests mucus, epithelial cells, and debris in sputum, while preserving the chitin-rich fungal cell walls, making them appear clear, …

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Fungal Elements in KOH Mount of BAL (Bronchoalveolar Lavage) Microscopy- Introduction, Fungal Elements Observed in BAL KOH Mount, Applications, and Keynotes

Fungal Elements in wet mount of culture of BAL specimen

Introduction KOH mount (Potassium Hydroxide preparation) is a rapid, simple, and cost-effective microscopic technique used to detect fungal elements directly in clinical specimens.When applied to Bronchoalveolar Lavage (BAL) samples, it is an essential diagnostic tool for identifying pulmonary fungal infections, especially in the ICU, oncology, …

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Fungal Elements in KOH Mount of Urine Microscopy: Introduction, Fungal Elements Observed in Urine KOH Mount, Applications, and Keynotes

Fungal elements in KOH Mount of Urine Microscopy- Introduction, Fungal Elements Observed in Urine KOH Mount, Applications, and Keynotes

Introduction Potassium Hydroxide (KOH) mount is a simple, rapid, and cost-effective microscopic technique used to detect fungal elements in various clinical specimens, including urine samples. When fungal infection of the urinary tract (funguria) is suspected—especially in immunocompromised, diabetic, or catheterized patients—KOH mount helps visualize yeast …

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Bio-Incubator: Introduction, Application, and Keynotes

This Bio-Incubator provides a stable environment for the growth and incubation of microbial cultures, enzymatic reactions, and biological assays. The display screen shows the process status as “FINISHED” with a temperature reading of 27.2°C, indicating the incubation cycle is complete. It is commonly used in clinical, molecular, and research laboratories for applications such as culture incubation, spore testing, and enzyme activation.

Introduction A Bio-Incubator is a laboratory device designed to provide a controlled environment of temperature, humidity, and time for the growth and maintenance of microbial cultures, cell lines, and biological reactions. It is an essential tool in microbiology, molecular biology, biotechnology, and clinical diagnostics.By maintaining …

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Molds-Broth Microdilution Testing (EUCAST): Introduction, Principle, Clinical Significance, and Keynotes

Molds-Broth Microdilution Testing (EUCAST)- Introduction, Principle, Clinical Significance, and Keynotes

Introduction Invasive mold infections caused by species such as Aspergillus, Fusarium, Scedosporium, and Mucorales pose serious challenges, especially in immunocompromised and oncology patients. Accurate antifungal susceptibility testing (AFST) is essential for guiding therapy and monitoring resistance trends. The EUCAST (European Committee on Antimicrobial Susceptibility Testing) …

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