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Introduction Bronchoalveolar lavage (BAL) is a diagnostic procedure used to collect specimens from the lower respiratory tract, particularly from the alveolar spaces. It is performed by instilling sterile saline into a segment of the lung via a bronchoscope and then aspirating it back. Gram staining …
Introduction Acinetobacter ursingii is a Gram-negative, non-fermentative, opportunistic bacterium belonging to the genus Acinetobacter. It is an uncommon species compared to Acinetobacter baumannii, but it has been increasingly recognized in healthcare-associated infections (HAIs). Reported cases include bacteremia, septicemia, pneumonia, urinary tract infections, and bloodstream infections, …
Introduction A biphasic medium is a culture system that combines both liquid (broth) and solid (agar) phases in the same container (usually a bottle or tube). It is designed to enhance microbial recovery by leveraging the advantages of both media types in one setup. Applications …
Introduction Acinetobacter baumannii is a Gram-negative, non-fermenting, aerobic coccobacillus. It is an opportunistic pathogen increasingly associated with nosocomial infections, particularly in critically ill or immunocompromised patients. Notorious for its antibiotic resistance, it has become a major global health concern, particularly in ICU settings. Morphology Pathogenicity …
Introduction Small Colony Variants (SCVs) are a subpopulation of bacteria that grow slowly and form unusually small colonies on culture media. They are most commonly described in Staphylococcus aureus, but also occur in Pseudomonas aeruginosa, Escherichia coli, and Listeria monocytogenes. SCVs are associated with chronic, …
Introduction Acinetobacter baumannii complex is a group of Gram-negative, non-fermenting bacilli known for causing healthcare-associated infections. It includes closely related species like A. baumannii, A. pittii, A. nosocomialis, and A. calcoaceticus. This complex thrives in hospital environments and poses a serious challenge due to its …
Introduction Proteus hauseri is a Gram-negative, facultatively anaerobic bacillus in the Enterobacteriaceae family. It is closely related to other Proteus species and commonly found in soil, water, and the human gastrointestinal tract. Though less prevalent than P. mirabilis, P. hauseri can cause opportunistic infections—especially in …
Introduction The Enterobacter cloacae complex (ECC) is a group of closely related bacterial species, including Enterobacter cloacae, Enterobacter hormaechei, and others, that can cause a variety of infections, particularly in healthcare settings. ECC is a significant cause of nosocomial (hospital-acquired) infections, including bloodstream infections, pneumonia, and urinary …