This site is fully related to medical laboratory information for public and health care professionals.
Introduction Tuberculosis (TB) is a contagious bacterial infection that primarily affects the lungs, although it can also impact other parts of the body. It is caused by Mycobacterium tuberculosis, a type of bacteria that spreads through the air when an infected person coughs, sneezes, or …
Introduction Pleural fluid culture and sensitivity is a diagnostic test used to identify and determine the antibiotic susceptibility of microorganisms present in the pleural fluid. The pleural fluid is a clear fluid that accumulates between the two layers of the pleura, the membrane that surrounds …
Introduction Mycobacterium tuberculosis is a bacterium that causes tuberculosis (TB), a contagious infectious disease primarily affecting the lungs but can also affect other parts of the body. TB has been a significant public health concern worldwide, causing illness and death for thousands of years. Key …
Introduction Sputum culture and sensitivity is a medical laboratory test used to identify and determine the susceptibility of bacteria or other microorganisms present in a patient’s sputum, a mixture of saliva and mucus coughed up from the respiratory tract. This diagnostic test is crucial in …
Introduction TB PCR (polymerase chain reaction) for sputum is a laboratory test used in the diagnosis of tuberculosis (TB). It is a molecular diagnostic technique that detects the presence of Mycobacterium tuberculosis (the bacterium that causes TB) DNA in sputum samples. Traditional methods of diagnosing …
Introduction of Lowenstein-Jensen (LJ) Medium Lowenstein-Jensen (LJ) Medium is named after the surnames of Austrian pathologist Ernst Lowenstein (1878–1950) and the Danish chemist Kai Arne Jensen (1908–1992). The etiological agent of tuberculosis is Mycobacterium tuberculosis. It requires aerobic conditions and a protein-enriched medium for culture. The short …
Introduction of Auramine Staining Auramine is a fluorochrome stain used to visualize acid-fast structures of various microorganisms especially Mycobacterium tuberculosis and in modified form for Mycobacterium leprae, Nocardia species, Cryptosporidium parvum, Cyclospora cayetanensis , Isospora belli, and fungal spores. Ziehl-Neelsen (hot), and Kinyoun (cold) are …
Introduction of Gram Positive Rods (GPR) ‘Gram Positive Bacilli (GPB)‘ is also called Gram Positive Rods (GPR) bacteria which retain crystal violet dye and stain blue or purple on Gram’s staining. The most common medically important bacteria of GPR are Mycobacterium tuberculosis, Mycobacterium leprae, Listeria monocytogenes, …