Mycobacterium tuberculosis Archives - Page 3 of 3

Lowenstein-Jensen (LJ) Medium: Introduction, Principle Composition, Preparation, Procedure, Result- Interpretation, Limitation, and Keynotes

November 30, 2022July 18, 2022 by Medical Lab Notes

Introduction of Lowenstein-Jensen (LJ) Medium Lowenstein-Jensen (LJ) Medium is named after the surnames of Austrian pathologist Ernst Lowenstein (1878–1950) and the Danish chemist Kai Arne Jensen (1908–1992). The etiological agent of tuberculosis is Mycobacterium tuberculosis. It requires aerobic conditions and a protein-enriched medium for culture. The short …

Auramine Staining: Introduction, Principle, Procedure, Result Interpretation, Limitations, Keynotes, and Related Footages

December 8, 2022March 20, 2022 by Medical Lab Notes

Introduction of Auramine Staining Auramine is a fluorochrome stain used to visualize acid-fast structures of various microorganisms especially Mycobacterium tuberculosis and in modified form for Mycobacterium leprae, Nocardia species, Cryptosporidium parvum, Cyclospora cayetanensis , Isospora belli, and fungal spores. Ziehl-Neelsen (hot), and Kinyoun (cold) are …

Gram Positive Bacilli or Rods: Introduction, List of Bacteria, Keynotes, and Related Pictures

December 14, 2022February 22, 2022 by Medical Lab Notes

Introduction of Gram Positive Rods (GPR) ‘Gram Positive Bacilli (GPB)‘ is also called Gram Positive Rods (GPR) bacteria which retain crystal violet dye and stain blue or purple on Gram’s staining. The most common medically important bacteria of GPR are Mycobacterium tuberculosis, Mycobacterium leprae, Listeria monocytogenes, …

Methylene Blue Stain: Introduction, Principle, Composition, Preparation, Staining Requirements, Staining Procedure, Result Interpretation, and Keynotes

December 6, 2022January 12, 2022 by Medical Lab Notes

Cryrptococcus neoformans capsules in Methylene blue wet mount Microscopy

Introduction Methylene Blue Stain, also known as methylene blue aqueous is an ordinary stain. It is used for capsule stain as well as simple staining for a rapid and easy way to determine cell shape, size, and arrangements of bacteria. It is also made easier to …

Ziehl -Neelsen Stain: Introduction, Principle, Test Requirements, Staining Procedure, Result Interpretation, Keynotes, and Z-N Stained Smear Footages Collections

December 7, 2022January 10, 2022 by Medical Lab Notes

Ziehl -Neelsen Stain Ziehl–Neelsen stain is a type of acid-fast stain and it is named after the surnames of two German doctors who modified the stain. One is the bacteriologist Franz Ziehl (1859–1926) and other is the pathologist Friedrich Neelsen (1854–1898). It was initially introduced …